Mitigating depth-of-field limitations in high-resolution ptychographic X-ray tomography
(2026) In Optics Express 34(16). p.28959-28971- Abstract
X-ray ptychographic tomography enables quantitative 3D imaging at nanometer resolution, but the limited depth of field causes out-of-focus artifacts in 2D reconstructions of extended samples, restricting the range of projection angles and reducing the quality of the reconstructed volume. Here, we present an imaging-plane-optimized ptychographic tomography method that corrects out-of-focus artefacts by optimizing the effective propagation distance during reconstruction. Both simulations and experiments show improved 2D image quality. Applied to tomography data, the method improves the quality of high-tilt projections and expands the angular range usable for reconstruction, thereby reducing axial elongation caused by the missing wedge. In... (More)
X-ray ptychographic tomography enables quantitative 3D imaging at nanometer resolution, but the limited depth of field causes out-of-focus artifacts in 2D reconstructions of extended samples, restricting the range of projection angles and reducing the quality of the reconstructed volume. Here, we present an imaging-plane-optimized ptychographic tomography method that corrects out-of-focus artefacts by optimizing the effective propagation distance during reconstruction. Both simulations and experiments show improved 2D image quality. Applied to tomography data, the method improves the quality of high-tilt projections and expands the angular range usable for reconstruction, thereby reducing axial elongation caused by the missing wedge. In the demonstrated macrophage dataset, this enables visualization of labeled intracellular structures and measurement of the distance between the silver label surface and the mitochondrion surface.
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- author
- Xiao, Jingcheng ; Chen, Nuo ; Zhang, Enci ; Chen, Hongyu ; He, Bo LU ; Yuan, Xinye ; Zhang, Difei ; Wen, Pingping ; Zhang, Jianhua and Jiang, Huaidong
- organization
- publishing date
- 2026-08-10
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Optics Express
- volume
- 34
- issue
- 16
- pages
- 13 pages
- publisher
- Optical Society of America
- external identifiers
-
- scopus:105047664413
- ISSN
- 1094-4087
- DOI
- 10.1364/OE.597588
- language
- English
- LU publication?
- yes
- id
- 8c6a179b-6efa-42f0-a93c-af21cf391c24
- date added to LUP
- 2026-10-01 10:47:15
- date last changed
- 2026-10-01 10:47:35
@article{8c6a179b-6efa-42f0-a93c-af21cf391c24,
abstract = {{<p>X-ray ptychographic tomography enables quantitative 3D imaging at nanometer resolution, but the limited depth of field causes out-of-focus artifacts in 2D reconstructions of extended samples, restricting the range of projection angles and reducing the quality of the reconstructed volume. Here, we present an imaging-plane-optimized ptychographic tomography method that corrects out-of-focus artefacts by optimizing the effective propagation distance during reconstruction. Both simulations and experiments show improved 2D image quality. Applied to tomography data, the method improves the quality of high-tilt projections and expands the angular range usable for reconstruction, thereby reducing axial elongation caused by the missing wedge. In the demonstrated macrophage dataset, this enables visualization of labeled intracellular structures and measurement of the distance between the silver label surface and the mitochondrion surface.</p>}},
author = {{Xiao, Jingcheng and Chen, Nuo and Zhang, Enci and Chen, Hongyu and He, Bo and Yuan, Xinye and Zhang, Difei and Wen, Pingping and Zhang, Jianhua and Jiang, Huaidong}},
issn = {{1094-4087}},
language = {{eng}},
month = {{08}},
number = {{16}},
pages = {{28959--28971}},
publisher = {{Optical Society of America}},
series = {{Optics Express}},
title = {{Mitigating depth-of-field limitations in high-resolution ptychographic X-ray tomography}},
url = {{http://dx.doi.org/10.1364/OE.597588}},
doi = {{10.1364/OE.597588}},
volume = {{34}},
year = {{2026}},
}