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The Escherichia coli CysZ is a pH dependent sulfate transporter that can be inhibited by sulfite

Zhang, Li; Jiang, Wangshu; Nan, Jie LU ; Almqvist, Jonas and Huang, Yafei (2014) In Biochimica et Biophysica Acta 1838(7). p.16-1809
Abstract

The Escherichia coli inner membrane protein CysZ mediates the sulfate uptake subsequently utilized for the synthesis of sulfur-containing compounds in cells. Here we report the purification and functional characterization of CysZ. Using Isothermal Titration Calorimetry, we have observed interactions between CysZ and its putative substrate sulfate. Additional sulfur-containing compounds from the cysteine synthesis pathway have also been analyzed for their abilities to interact with CysZ. Our results suggest that CysZ is dedicated to a specific pathway that assimilates sulfate for the synthesis of cysteine. Sulfate uptake via CysZ into E. coli whole cells and proteoliposome offers direct evidence of CysZ being able to mediate sulfate... (More)

The Escherichia coli inner membrane protein CysZ mediates the sulfate uptake subsequently utilized for the synthesis of sulfur-containing compounds in cells. Here we report the purification and functional characterization of CysZ. Using Isothermal Titration Calorimetry, we have observed interactions between CysZ and its putative substrate sulfate. Additional sulfur-containing compounds from the cysteine synthesis pathway have also been analyzed for their abilities to interact with CysZ. Our results suggest that CysZ is dedicated to a specific pathway that assimilates sulfate for the synthesis of cysteine. Sulfate uptake via CysZ into E. coli whole cells and proteoliposome offers direct evidence of CysZ being able to mediate sulfate uptake. In addition, the cysteine synthesis pathway intermediate sulfite can interact directly with CysZ with higher affinity than sulfate. The sulfate transport activity is inhibited in the presence of sulfite, suggesting the existence of a feedback inhibition mechanism in which sulfite regulates sulfate uptake by CysZ. Sulfate uptake assays performed at different extracellular pH and in the presence of a proton uncoupler indicate that this uptake is driven by the proton gradient.

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author
organization
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type
Contribution to journal
publication status
published
keywords
Biological Transport, Cysteine, Escherichia coli, Escherichia coli Proteins, Hydrogen-Ion Concentration, Membrane Proteins, Proteolipids, Protons, Sulfates
in
Biochimica et Biophysica Acta
volume
1838
issue
7
pages
8 pages
publisher
Elsevier
external identifiers
  • scopus:84899038774
ISSN
0006-3002
DOI
10.1016/j.bbamem.2014.03.003
language
English
LU publication?
yes
id
8c8aec9e-48da-40a7-a7b8-eb688a0223e0
date added to LUP
2016-09-07 22:49:52
date last changed
2017-04-09 04:48:34
@article{8c8aec9e-48da-40a7-a7b8-eb688a0223e0,
  abstract     = {<p>The Escherichia coli inner membrane protein CysZ mediates the sulfate uptake subsequently utilized for the synthesis of sulfur-containing compounds in cells. Here we report the purification and functional characterization of CysZ. Using Isothermal Titration Calorimetry, we have observed interactions between CysZ and its putative substrate sulfate. Additional sulfur-containing compounds from the cysteine synthesis pathway have also been analyzed for their abilities to interact with CysZ. Our results suggest that CysZ is dedicated to a specific pathway that assimilates sulfate for the synthesis of cysteine. Sulfate uptake via CysZ into E. coli whole cells and proteoliposome offers direct evidence of CysZ being able to mediate sulfate uptake. In addition, the cysteine synthesis pathway intermediate sulfite can interact directly with CysZ with higher affinity than sulfate. The sulfate transport activity is inhibited in the presence of sulfite, suggesting the existence of a feedback inhibition mechanism in which sulfite regulates sulfate uptake by CysZ. Sulfate uptake assays performed at different extracellular pH and in the presence of a proton uncoupler indicate that this uptake is driven by the proton gradient.</p>},
  author       = {Zhang, Li and Jiang, Wangshu and Nan, Jie and Almqvist, Jonas and Huang, Yafei},
  issn         = {0006-3002},
  keyword      = {Biological Transport,Cysteine,Escherichia coli,Escherichia coli Proteins,Hydrogen-Ion Concentration,Membrane Proteins,Proteolipids,Protons,Sulfates},
  language     = {eng},
  number       = {7},
  pages        = {16--1809},
  publisher    = {Elsevier},
  series       = {Biochimica et Biophysica Acta},
  title        = {The Escherichia coli CysZ is a pH dependent sulfate transporter that can be inhibited by sulfite},
  url          = {http://dx.doi.org/10.1016/j.bbamem.2014.03.003},
  volume       = {1838},
  year         = {2014},
}