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Cryo-EM Reveals Promoter DNA Binding and Conformational Flexibility of the General Transcription Factor TFIID

Elmlund, Hans ; Baraznenok, Vera ; Linder, Tomas ; Szilagyi, Zsolt ; Rofougaran, Reza ; Hofer, Anders ; Hebert, Hans LU ; Lindahl, Martin LU and Gustafsson, Claes M. (2009) In Structure 17(11). p.1442-1452
Abstract
The general transcription factor IID (TFIID) is required for initiation of RNA polymerase II-dependent transcription at many eukaryotic promoters. TFIID comprises the TATA-binding protein (TBP) and several conserved TBP-associated factors (TAFs). Recognition of the core promoter by TFIID assists assembly of the preinitiation complex. Using cryo-electron microscopy in combination with methods for ab initio single-particle reconstruction and heterogeneity analysis, we have produced density maps of two conformational states of Schizosaccharomyces pombe TFIID, containing and lacking TBP. We report that TBP-binding is coupled to a massive histone-fold domain rearrangement. Moreover, docking of the TBP-TAF1(N-terminus) atomic structure to the... (More)
The general transcription factor IID (TFIID) is required for initiation of RNA polymerase II-dependent transcription at many eukaryotic promoters. TFIID comprises the TATA-binding protein (TBP) and several conserved TBP-associated factors (TAFs). Recognition of the core promoter by TFIID assists assembly of the preinitiation complex. Using cryo-electron microscopy in combination with methods for ab initio single-particle reconstruction and heterogeneity analysis, we have produced density maps of two conformational states of Schizosaccharomyces pombe TFIID, containing and lacking TBP. We report that TBP-binding is coupled to a massive histone-fold domain rearrangement. Moreover, docking of the TBP-TAF1(N-terminus) atomic structure to the THID map and reconstruction of a TAF-promoter DNA complex helps to account for TAF-dependent regulation of promoter-TBP and promoter-TAF interactions. (Less)
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author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Structure
volume
17
issue
11
pages
1442 - 1452
publisher
Cell Press
external identifiers
  • wos:000272011500006
  • scopus:70350707312
  • pmid:19913479
ISSN
0969-2126
DOI
10.1016/j.str.2009.09.007
language
English
LU publication?
yes
id
8c8b8213-2370-4310-8591-a36c49fec5e5 (old id 1517917)
date added to LUP
2016-04-01 11:36:37
date last changed
2025-10-14 11:04:52
@article{8c8b8213-2370-4310-8591-a36c49fec5e5,
  abstract     = {{The general transcription factor IID (TFIID) is required for initiation of RNA polymerase II-dependent transcription at many eukaryotic promoters. TFIID comprises the TATA-binding protein (TBP) and several conserved TBP-associated factors (TAFs). Recognition of the core promoter by TFIID assists assembly of the preinitiation complex. Using cryo-electron microscopy in combination with methods for ab initio single-particle reconstruction and heterogeneity analysis, we have produced density maps of two conformational states of Schizosaccharomyces pombe TFIID, containing and lacking TBP. We report that TBP-binding is coupled to a massive histone-fold domain rearrangement. Moreover, docking of the TBP-TAF1(N-terminus) atomic structure to the THID map and reconstruction of a TAF-promoter DNA complex helps to account for TAF-dependent regulation of promoter-TBP and promoter-TAF interactions.}},
  author       = {{Elmlund, Hans and Baraznenok, Vera and Linder, Tomas and Szilagyi, Zsolt and Rofougaran, Reza and Hofer, Anders and Hebert, Hans and Lindahl, Martin and Gustafsson, Claes M.}},
  issn         = {{0969-2126}},
  language     = {{eng}},
  number       = {{11}},
  pages        = {{1442--1452}},
  publisher    = {{Cell Press}},
  series       = {{Structure}},
  title        = {{Cryo-EM Reveals Promoter DNA Binding and Conformational Flexibility of the General Transcription Factor TFIID}},
  url          = {{http://dx.doi.org/10.1016/j.str.2009.09.007}},
  doi          = {{10.1016/j.str.2009.09.007}},
  volume       = {{17}},
  year         = {{2009}},
}