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Rational Design of Bright Long Fluorescence Lifetime Dyad Fluorophores for Single Molecule Imaging and Detection

Kacenauskaite, Laura ; Bisballe, Niels ; Mucci, Rebecca ; Santella, Marco ; Pullerits, Tönu LU ; Chen, Junsheng LU ; Vosch, Tom and Laursen, Bo W. (2021) In Journal of the American Chemical Society p.1377-1385
Abstract

Increasing demand for detecting single molecules in challenging environments has raised the bar for the fluorophores used. To achieve better resolution and/or contrast in fluorescence microscopy, it is now essential to use bright and stable dyes with tailored photophysical properties. While long fluorescence lifetime fluorophores offer many advantages in time-resolved imaging, their inherently lower molar absorption coefficient has limited applications in single molecule imaging. Here we propose a generic approach to prepare bright, long fluorescence lifetime dyad fluorophores comprising an absorbing antenna chromophore with high absorption coefficient linked to an acceptor emitter with a long fluorescence lifetime. We introduce a dyad... (More)

Increasing demand for detecting single molecules in challenging environments has raised the bar for the fluorophores used. To achieve better resolution and/or contrast in fluorescence microscopy, it is now essential to use bright and stable dyes with tailored photophysical properties. While long fluorescence lifetime fluorophores offer many advantages in time-resolved imaging, their inherently lower molar absorption coefficient has limited applications in single molecule imaging. Here we propose a generic approach to prepare bright, long fluorescence lifetime dyad fluorophores comprising an absorbing antenna chromophore with high absorption coefficient linked to an acceptor emitter with a long fluorescence lifetime. We introduce a dyad consisting of a perylene antenna and a triangulenium emitter with 100% energy transfer from donor to acceptor. The dyad retained the long fluorescence lifetime (17 ns) and high quantum yield (75%) of the triangulenium emitter, while the perylene antenna increased the molar absorption coefficient (up to 5 times) in comparison to the free triangulenium dye. These triangulenium based dyads with significantly improved brightness can now be detected at the single molecule level and easily discriminated from bright autofluorescence by time-gated and other lifetime-based detection schemes.

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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of the American Chemical Society
pages
1377 - 1385
publisher
The American Chemical Society (ACS)
external identifiers
  • pmid:33427468
  • scopus:85099913602
ISSN
0002-7863
DOI
10.1021/jacs.0c10457
language
English
LU publication?
yes
id
8c9bf4ca-d8b6-49bf-9bf7-c2daacd9bed2
date added to LUP
2021-02-08 11:32:39
date last changed
2021-03-24 02:28:35
@article{8c9bf4ca-d8b6-49bf-9bf7-c2daacd9bed2,
  abstract     = {<p>Increasing demand for detecting single molecules in challenging environments has raised the bar for the fluorophores used. To achieve better resolution and/or contrast in fluorescence microscopy, it is now essential to use bright and stable dyes with tailored photophysical properties. While long fluorescence lifetime fluorophores offer many advantages in time-resolved imaging, their inherently lower molar absorption coefficient has limited applications in single molecule imaging. Here we propose a generic approach to prepare bright, long fluorescence lifetime dyad fluorophores comprising an absorbing antenna chromophore with high absorption coefficient linked to an acceptor emitter with a long fluorescence lifetime. We introduce a dyad consisting of a perylene antenna and a triangulenium emitter with 100% energy transfer from donor to acceptor. The dyad retained the long fluorescence lifetime (17 ns) and high quantum yield (75%) of the triangulenium emitter, while the perylene antenna increased the molar absorption coefficient (up to 5 times) in comparison to the free triangulenium dye. These triangulenium based dyads with significantly improved brightness can now be detected at the single molecule level and easily discriminated from bright autofluorescence by time-gated and other lifetime-based detection schemes.</p>},
  author       = {Kacenauskaite, Laura and Bisballe, Niels and Mucci, Rebecca and Santella, Marco and Pullerits, Tönu and Chen, Junsheng and Vosch, Tom and Laursen, Bo W.},
  issn         = {0002-7863},
  language     = {eng},
  pages        = {1377--1385},
  publisher    = {The American Chemical Society (ACS)},
  series       = {Journal of the American Chemical Society},
  title        = {Rational Design of Bright Long Fluorescence Lifetime Dyad Fluorophores for Single Molecule Imaging and Detection},
  url          = {http://dx.doi.org/10.1021/jacs.0c10457},
  doi          = {10.1021/jacs.0c10457},
  year         = {2021},
}