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Changes in renal microcirculation induced by infusion of (fe3)- and (fe2)-myoglobin during hemorrhagic hypotension in the anesthetized rat : Influence of l-name and 8-br-cyclic gMP

Vetterlein, Friedrich ; Reimers, Arne LU orcid and Schmidt, Gerhard (1996) In Nephron 73(2). p.243-250
Abstract

The effects of myoglobin on renal microcirculation were studied in anesthetized rats subjected to hemorrhagic hypotension. Capillary flow distribution was determined by allowing two dyes to circulate for 3 and 1 min, respectively, freezing the left kidney and quantifying the dye distribution in histological sections by analyzing the distances of regularly spaced test points to the next dye-labeled capillary. Control experiments showed 88% of distances to be<12 μm in the cortex [medullary outer stripe (OS): 77%, inner stripe (IS): 93%] and no distance to be > 60 μm. Myoglobin induced disturbances in intra-renal perfusion with a significantly higher potency of (Fe2+)- as compared to (Fe3+)-myoglobin. With the... (More)

The effects of myoglobin on renal microcirculation were studied in anesthetized rats subjected to hemorrhagic hypotension. Capillary flow distribution was determined by allowing two dyes to circulate for 3 and 1 min, respectively, freezing the left kidney and quantifying the dye distribution in histological sections by analyzing the distances of regularly spaced test points to the next dye-labeled capillary. Control experiments showed 88% of distances to be<12 μm in the cortex [medullary outer stripe (OS): 77%, inner stripe (IS): 93%] and no distance to be > 60 μm. Myoglobin induced disturbances in intra-renal perfusion with a significantly higher potency of (Fe2+)- as compared to (Fe3+)-myoglobin. With the reduced species, the fraction of distances > 60 μm increased to 54% in the cortex (OS: 69%; IS: 67%). L-NAME, an inhibitor of nitric oxide synthesis, induced similar defects of perfusion. The cGMP analogue 8-Br-cGMP was able to nearly completely prevent these effects. The results support the view that myoglobin when released during hemorrhagic hypotension impairs renal microcirculation supposedly by scavenging the endogenous relaxing factor nitric oxide.

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author
; and
publishing date
type
Contribution to journal
publication status
published
keywords
Cyclic GMP, Microcirculation, Myoglobin, Nitric oxide, Renal failure
in
Nephron
volume
73
issue
2
pages
243 - 250
publisher
Karger
external identifiers
  • scopus:0029894947
  • pmid:8773351
ISSN
1660-8151
DOI
10.1159/000189047
language
English
LU publication?
no
id
8cb03263-0497-4215-b217-6211b7321dde
date added to LUP
2024-08-31 15:00:43
date last changed
2025-01-05 05:29:23
@article{8cb03263-0497-4215-b217-6211b7321dde,
  abstract     = {{<p>The effects of myoglobin on renal microcirculation were studied in anesthetized rats subjected to hemorrhagic hypotension. Capillary flow distribution was determined by allowing two dyes to circulate for 3 and 1 min, respectively, freezing the left kidney and quantifying the dye distribution in histological sections by analyzing the distances of regularly spaced test points to the next dye-labeled capillary. Control experiments showed 88% of distances to be&lt;12 μm in the cortex [medullary outer stripe (OS): 77%, inner stripe (IS): 93%] and no distance to be &gt; 60 μm. Myoglobin induced disturbances in intra-renal perfusion with a significantly higher potency of (Fe<sup>2+</sup>)- as compared to (Fe<sup>3+</sup>)-myoglobin. With the reduced species, the fraction of distances &gt; 60 μm increased to 54% in the cortex (OS: 69%; IS: 67%). L-NAME, an inhibitor of nitric oxide synthesis, induced similar defects of perfusion. The cGMP analogue 8-Br-cGMP was able to nearly completely prevent these effects. The results support the view that myoglobin when released during hemorrhagic hypotension impairs renal microcirculation supposedly by scavenging the endogenous relaxing factor nitric oxide.</p>}},
  author       = {{Vetterlein, Friedrich and Reimers, Arne and Schmidt, Gerhard}},
  issn         = {{1660-8151}},
  keywords     = {{Cyclic GMP; Microcirculation; Myoglobin; Nitric oxide; Renal failure}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{243--250}},
  publisher    = {{Karger}},
  series       = {{Nephron}},
  title        = {{Changes in renal microcirculation induced by infusion of (fe<sup>3</sup>)- and (fe<sup>2</sup>)-myoglobin during hemorrhagic hypotension in the anesthetized rat : Influence of l-name and 8-br-cyclic gMP}},
  url          = {{http://dx.doi.org/10.1159/000189047}},
  doi          = {{10.1159/000189047}},
  volume       = {{73}},
  year         = {{1996}},
}