Associations between APOE genotypes, urine 8-isoprostane and blood trace elements in middle-aged mothers (CROME study)
(2024) In Environment International 193. p.1-14- Abstract
Background: There is almost no data on the combined associations between apolipoprotein E gene (APOE) genotypes, trace elements (TEs), and lipid peroxidation in vivo. The aim of our study was to evaluate the association between APOE genotypes and TE levels in blood (B-TEs) and erythrocytes (E-TEs), and 8-isoprostane in urine (U-8-isoprostane) in women with low exposure to potentially toxic TEs and with adequate supply of essential TEs. Methods: B-TEs, E-TEs and U-8-isoprostane were determined in 172 healthy women of childbearing age (30.1–51.4 years) using ICP-MS and ELISA competitive assay, respectively. All women were divided into three APOE genotype groups according to the presence of the ɛ4 allele, ɛ2 allele or ɛ3 homozygotic... (More)
Background: There is almost no data on the combined associations between apolipoprotein E gene (APOE) genotypes, trace elements (TEs), and lipid peroxidation in vivo. The aim of our study was to evaluate the association between APOE genotypes and TE levels in blood (B-TEs) and erythrocytes (E-TEs), and 8-isoprostane in urine (U-8-isoprostane) in women with low exposure to potentially toxic TEs and with adequate supply of essential TEs. Methods: B-TEs, E-TEs and U-8-isoprostane were determined in 172 healthy women of childbearing age (30.1–51.4 years) using ICP-MS and ELISA competitive assay, respectively. All women were divided into three APOE genotype groups according to the presence of the ɛ4 allele, ɛ2 allele or ɛ3 homozygotic allele. The associations between B-TEs, E-TE, U-8-isoprostane, and the APOE genotype groups were estimated by multiple variable linear regression models with relevant explanatory variables (e.g., age, BMI, and seafood). Results: All TE and U-8-isoprostane levels were inside the reference ranges for the healthy population. In the multiple variable linear regression models, our results showed that urine 8-isoprostane levels increased by up to 43.3% in the APOE4 group compared to the APOE3 group and a negligible negative modifying effect for essential TEs. However, the APOE genotype groups were associated also with some TEs. A clear positive association was found between the APOE2 and APOE4 groups (vs. APOE3) with B-molybdenum. Conclusions: Our study suggests that the APOE4 genotype played an important role in 8-isoprostane variability in a population with an adequate supply of essential and with low exposure to potentially toxic TEs. Adequate copper, zinc and selenium status seemed to be protective against, while the levels of nonessential TEs were probably too low to play a decisive role in 8-isoprostane formation. The observed impact of the APOE2 and APOE4 groups on increased B-molybdenum opens a new research topic.
(Less)
- author
- France Štiglic, Alenka ; Stajnko, Anja LU ; Sešek Briški, Alenka ; Snoj Tratnik, Janja ; Mazej, Darja ; Jerin, Aleš ; Skitek, Milan ; Horvat, Milena ; Marc, Janja and Falnoga, Ingrid
- publishing date
- 2024-11
- type
- Contribution to journal
- publication status
- published
- keywords
- APOE alleles, Human population, Lipid peroxidation status, Multiple variable linear regression models, Trace elements
- in
- Environment International
- volume
- 193
- article number
- 109034
- pages
- 1 - 14
- publisher
- Elsevier
- external identifiers
-
- scopus:85207032535
- pmid:39447471
- ISSN
- 0160-4120
- DOI
- 10.1016/j.envint.2024.109034
- language
- English
- LU publication?
- no
- additional info
- Publisher Copyright: © 2024 The Author(s)
- id
- 8d4bc962-7dbd-4b4c-b7e5-aab5f77eb6b8
- date added to LUP
- 2025-02-21 21:57:19
- date last changed
- 2025-07-12 09:39:51
@article{8d4bc962-7dbd-4b4c-b7e5-aab5f77eb6b8, abstract = {{<p>Background: There is almost no data on the combined associations between apolipoprotein E gene (APOE) genotypes, trace elements (TEs), and lipid peroxidation in vivo. The aim of our study was to evaluate the association between APOE genotypes and TE levels in blood (B-TEs) and erythrocytes (E-TEs), and 8-isoprostane in urine (U-8-isoprostane) in women with low exposure to potentially toxic TEs and with adequate supply of essential TEs. Methods: B-TEs, E-TEs and U-8-isoprostane were determined in 172 healthy women of childbearing age (30.1–51.4 years) using ICP-MS and ELISA competitive assay, respectively. All women were divided into three APOE genotype groups according to the presence of the ɛ4 allele, ɛ2 allele or ɛ3 homozygotic allele. The associations between B-TEs, E-TE, U-8-isoprostane, and the APOE genotype groups were estimated by multiple variable linear regression models with relevant explanatory variables (e.g., age, BMI, and seafood). Results: All TE and U-8-isoprostane levels were inside the reference ranges for the healthy population. In the multiple variable linear regression models, our results showed that urine 8-isoprostane levels increased by up to 43.3% in the APOE4 group compared to the APOE3 group and a negligible negative modifying effect for essential TEs. However, the APOE genotype groups were associated also with some TEs. A clear positive association was found between the APOE2 and APOE4 groups (vs. APOE3) with B-molybdenum. Conclusions: Our study suggests that the APOE4 genotype played an important role in 8-isoprostane variability in a population with an adequate supply of essential and with low exposure to potentially toxic TEs. Adequate copper, zinc and selenium status seemed to be protective against, while the levels of nonessential TEs were probably too low to play a decisive role in 8-isoprostane formation. The observed impact of the APOE2 and APOE4 groups on increased B-molybdenum opens a new research topic.</p>}}, author = {{France Štiglic, Alenka and Stajnko, Anja and Sešek Briški, Alenka and Snoj Tratnik, Janja and Mazej, Darja and Jerin, Aleš and Skitek, Milan and Horvat, Milena and Marc, Janja and Falnoga, Ingrid}}, issn = {{0160-4120}}, keywords = {{APOE alleles; Human population; Lipid peroxidation status; Multiple variable linear regression models; Trace elements}}, language = {{eng}}, pages = {{1--14}}, publisher = {{Elsevier}}, series = {{Environment International}}, title = {{Associations between APOE genotypes, urine 8-isoprostane and blood trace elements in middle-aged mothers (CROME study)}}, url = {{http://dx.doi.org/10.1016/j.envint.2024.109034}}, doi = {{10.1016/j.envint.2024.109034}}, volume = {{193}}, year = {{2024}}, }