Derivation, characterization, and differentiation of human embryonic stem cells
(2004) In Stem Cells 22(3). p.367-376- Abstract
- The derivation of human embryonic stem (hES) cells establishes a new avenue to approach many issues in human biology and medicine for the first time. To meet the increased demand for characterized hES cell lines, we present the derivation and characterization of six hES cell lines. In addition to the previously described immunosurgery procedure, we were able to propagate the inner cell mass and establish hES cell lines from pronasetreated and hatched blastocysts. The cell lines were extensively characterized by expression analysis of markers gated in an undiffer characteristic for undifferentiated and differentiated hES cells, karyotyping, telomerase activity measurement, and pluripotency assays in vitro and in vivo. Whereas three of the... (More)
- The derivation of human embryonic stem (hES) cells establishes a new avenue to approach many issues in human biology and medicine for the first time. To meet the increased demand for characterized hES cell lines, we present the derivation and characterization of six hES cell lines. In addition to the previously described immunosurgery procedure, we were able to propagate the inner cell mass and establish hES cell lines from pronasetreated and hatched blastocysts. The cell lines were extensively characterized by expression analysis of markers gated in an undiffer characteristic for undifferentiated and differentiated hES cells, karyotyping, telomerase activity measurement, and pluripotency assays in vitro and in vivo. Whereas three of the cell lines expressed all the characteristics of undifferentiated pluripotent hES cells, one cell line carried a chromosome 13 trisomy while maintaining an undifferentiated pluripotent state, and two cell lines, one of which carried a triploid karyotype, exhibited limited pluripotency in vivo. Furthermore, we clonally derived one cell line, which could be propaentiated pluripotent state. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/275760
- author
- Heins, N ; Englund, MCO ; Sjoblom, C ; Dahl, U ; Tonning, A ; Bergh, C ; Lindahl, A ; Hanson, C and Semb, Henrik LU
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- differentiation, pluripotency, human ES cell, human blastocyst, ICM, subcloning
- in
- Stem Cells
- volume
- 22
- issue
- 3
- pages
- 367 - 376
- publisher
- Oxford University Press
- external identifiers
-
- wos:000221869600016
- pmid:15153613
- scopus:2942591062
- ISSN
- 1549-4918
- language
- English
- LU publication?
- no
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Stem Cell and Pancreas Developmental Biology (013212044)
- id
- 8d8138ef-683f-4f56-a3e6-43cc68930286 (old id 275760)
- alternative location
- http://stemcells.alphamedpress.org/cgi/reprint/22/3/367.pdf
- date added to LUP
- 2016-04-01 16:38:36
- date last changed
- 2023-02-23 01:47:17
@article{8d8138ef-683f-4f56-a3e6-43cc68930286,
abstract = {{The derivation of human embryonic stem (hES) cells establishes a new avenue to approach many issues in human biology and medicine for the first time. To meet the increased demand for characterized hES cell lines, we present the derivation and characterization of six hES cell lines. In addition to the previously described immunosurgery procedure, we were able to propagate the inner cell mass and establish hES cell lines from pronasetreated and hatched blastocysts. The cell lines were extensively characterized by expression analysis of markers gated in an undiffer characteristic for undifferentiated and differentiated hES cells, karyotyping, telomerase activity measurement, and pluripotency assays in vitro and in vivo. Whereas three of the cell lines expressed all the characteristics of undifferentiated pluripotent hES cells, one cell line carried a chromosome 13 trisomy while maintaining an undifferentiated pluripotent state, and two cell lines, one of which carried a triploid karyotype, exhibited limited pluripotency in vivo. Furthermore, we clonally derived one cell line, which could be propaentiated pluripotent state.}},
author = {{Heins, N and Englund, MCO and Sjoblom, C and Dahl, U and Tonning, A and Bergh, C and Lindahl, A and Hanson, C and Semb, Henrik}},
issn = {{1549-4918}},
keywords = {{differentiation; pluripotency; human ES cell; human blastocyst; ICM; subcloning}},
language = {{eng}},
number = {{3}},
pages = {{367--376}},
publisher = {{Oxford University Press}},
series = {{Stem Cells}},
title = {{Derivation, characterization, and differentiation of human embryonic stem cells}},
url = {{http://stemcells.alphamedpress.org/cgi/reprint/22/3/367.pdf}},
volume = {{22}},
year = {{2004}},
}