Re-assessment of YAP1 and MCR1 contributions to inhibitor tolerance in robust engineered Saccharomyces cerevisiae fermenting undetoxified lignocellulosic hydrolysate.
(2014) In AMB Express 4.- Abstract
- Development of robust yeast strains that can efficiently ferment lignocellulose-based feedstocks is one of the requirements for achieving economically feasible bioethanol production processes. With this goal, several genes have been identified as promising candidates to confer improved tolerance to S. cerevisiae. In most of the cases, however, the evaluation of the genetic modification was performed only in laboratory strains, that is, in strains that are known to be quite sensitive to various types of stresses. In the present study, we evaluated the effects of overexpressing genes encoding the transcription factor (YAP1) and the mitochondrial NADH-cytochrome b5 reductase (MCR1), either alone or in combination, in an already robust and... (More)
- Development of robust yeast strains that can efficiently ferment lignocellulose-based feedstocks is one of the requirements for achieving economically feasible bioethanol production processes. With this goal, several genes have been identified as promising candidates to confer improved tolerance to S. cerevisiae. In most of the cases, however, the evaluation of the genetic modification was performed only in laboratory strains, that is, in strains that are known to be quite sensitive to various types of stresses. In the present study, we evaluated the effects of overexpressing genes encoding the transcription factor (YAP1) and the mitochondrial NADH-cytochrome b5 reductase (MCR1), either alone or in combination, in an already robust and xylose-consuming industrial strain of S. cerevisiae and evaluated the effect during the fermentation of undiluted and undetoxified spruce hydrolysate. Overexpression of either gene resulted in faster hexose catabolism, but no cumulative effect was observed with the simultaneous overexpression. The improved phenotype of MCR1 overexpression appeared to be related, at least in part, to a faster furaldehyde reduction capacity, indicating that this reductase may have a wider substrate range than previously reported. Unexpectedly a decreased xylose fermentation rate was also observed in YAP1 overexpressing strains and possible reasons behind this phenotype are discussed. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4614174
- author
- Wallace, Valeria LU ; Signori, Lorenzo ; Li, Ying-Ying ; Ask, Magnus ; Bettiga, Maurizio ; Porro, Danilo ; Thevelein, Johan M ; Branduardi, Paola ; Foulquié-Moreno, María R and Gorwa-Grauslund, Marie-Francoise LU
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- AMB Express
- volume
- 4
- article number
- 56
- publisher
- Springer
- external identifiers
-
- pmid:25147754
- scopus:84947046745
- pmid:25147754
- wos:000358063300001
- ISSN
- 2191-0855
- DOI
- 10.1186/s13568-014-0056-5
- language
- English
- LU publication?
- yes
- id
- 8d885d69-7aca-4b52-825d-ca67f0043203 (old id 4614174)
- date added to LUP
- 2016-04-01 14:14:05
- date last changed
- 2022-04-22 02:07:10
@article{8d885d69-7aca-4b52-825d-ca67f0043203,
abstract = {{Development of robust yeast strains that can efficiently ferment lignocellulose-based feedstocks is one of the requirements for achieving economically feasible bioethanol production processes. With this goal, several genes have been identified as promising candidates to confer improved tolerance to S. cerevisiae. In most of the cases, however, the evaluation of the genetic modification was performed only in laboratory strains, that is, in strains that are known to be quite sensitive to various types of stresses. In the present study, we evaluated the effects of overexpressing genes encoding the transcription factor (YAP1) and the mitochondrial NADH-cytochrome b5 reductase (MCR1), either alone or in combination, in an already robust and xylose-consuming industrial strain of S. cerevisiae and evaluated the effect during the fermentation of undiluted and undetoxified spruce hydrolysate. Overexpression of either gene resulted in faster hexose catabolism, but no cumulative effect was observed with the simultaneous overexpression. The improved phenotype of MCR1 overexpression appeared to be related, at least in part, to a faster furaldehyde reduction capacity, indicating that this reductase may have a wider substrate range than previously reported. Unexpectedly a decreased xylose fermentation rate was also observed in YAP1 overexpressing strains and possible reasons behind this phenotype are discussed.}},
author = {{Wallace, Valeria and Signori, Lorenzo and Li, Ying-Ying and Ask, Magnus and Bettiga, Maurizio and Porro, Danilo and Thevelein, Johan M and Branduardi, Paola and Foulquié-Moreno, María R and Gorwa-Grauslund, Marie-Francoise}},
issn = {{2191-0855}},
language = {{eng}},
publisher = {{Springer}},
series = {{AMB Express}},
title = {{Re-assessment of YAP1 and MCR1 contributions to inhibitor tolerance in robust engineered Saccharomyces cerevisiae fermenting undetoxified lignocellulosic hydrolysate.}},
url = {{http://dx.doi.org/10.1186/s13568-014-0056-5}},
doi = {{10.1186/s13568-014-0056-5}},
volume = {{4}},
year = {{2014}},
}