Surface-Functionalized Polystyrene Nanoparticles Alter the Transmembrane Potential via Ion-Selective Pores Maintaining Global Bilayer Integrity
(2022) In Langmuir 38(48). p.14837-14849- Abstract
Although nanoplastics have well-known toxic effects toward the environment and living organisms, their molecular toxicity mechanisms, including the nature of nanoparticle-cell membrane interactions, are still under investigation. Here, we employ dynamic light scattering, quartz crystal microbalance with dissipation monitoring, and electrophysiology to investigate the interaction between polystyrene nanoparticles (PS NPs) and phospholipid membranes. Our results show that PS NPs adsorb onto lipid bilayers creating soft inhomogeneous films that include disordered defects. PS NPs form an integral part of the generated channels so that the surface functionalization and charge of the NP determine the pore conductive properties. The large... (More)
Although nanoplastics have well-known toxic effects toward the environment and living organisms, their molecular toxicity mechanisms, including the nature of nanoparticle-cell membrane interactions, are still under investigation. Here, we employ dynamic light scattering, quartz crystal microbalance with dissipation monitoring, and electrophysiology to investigate the interaction between polystyrene nanoparticles (PS NPs) and phospholipid membranes. Our results show that PS NPs adsorb onto lipid bilayers creating soft inhomogeneous films that include disordered defects. PS NPs form an integral part of the generated channels so that the surface functionalization and charge of the NP determine the pore conductive properties. The large difference in size between the NP diameter and the lipid bilayer thickness (∼60 vs ∼5 nm) suggests a particular and complex lipid-NP assembly that is able to maintain overall membrane integrity. In view of this, we suggest that NP-induced toxicity in cells could operate in more subtle ways than membrane disintegration, such as inducing lipid reorganization and transmembrane ionic fluxes that disrupt the membrane potential.
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- author
- Perini, D. Aurora ; Parra-Ortiz, Elisa ; Varó, Inmaculada ; Queralt-Martín, María ; Malmsten, Martin LU and Alcaraz, Antonio
- organization
- publishing date
- 2022-12
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Langmuir
- volume
- 38
- issue
- 48
- pages
- 13 pages
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- scopus:85143056201
- pmid:36417698
- ISSN
- 0743-7463
- DOI
- 10.1021/acs.langmuir.2c02487
- language
- English
- LU publication?
- yes
- id
- 8db88a18-943c-4df5-b826-c31b14b82a81
- date added to LUP
- 2022-12-27 10:47:12
- date last changed
- 2024-06-13 22:08:03
@article{8db88a18-943c-4df5-b826-c31b14b82a81,
abstract = {{<p>Although nanoplastics have well-known toxic effects toward the environment and living organisms, their molecular toxicity mechanisms, including the nature of nanoparticle-cell membrane interactions, are still under investigation. Here, we employ dynamic light scattering, quartz crystal microbalance with dissipation monitoring, and electrophysiology to investigate the interaction between polystyrene nanoparticles (PS NPs) and phospholipid membranes. Our results show that PS NPs adsorb onto lipid bilayers creating soft inhomogeneous films that include disordered defects. PS NPs form an integral part of the generated channels so that the surface functionalization and charge of the NP determine the pore conductive properties. The large difference in size between the NP diameter and the lipid bilayer thickness (∼60 vs ∼5 nm) suggests a particular and complex lipid-NP assembly that is able to maintain overall membrane integrity. In view of this, we suggest that NP-induced toxicity in cells could operate in more subtle ways than membrane disintegration, such as inducing lipid reorganization and transmembrane ionic fluxes that disrupt the membrane potential.</p>}},
author = {{Perini, D. Aurora and Parra-Ortiz, Elisa and Varó, Inmaculada and Queralt-Martín, María and Malmsten, Martin and Alcaraz, Antonio}},
issn = {{0743-7463}},
language = {{eng}},
number = {{48}},
pages = {{14837--14849}},
publisher = {{The American Chemical Society (ACS)}},
series = {{Langmuir}},
title = {{Surface-Functionalized Polystyrene Nanoparticles Alter the Transmembrane Potential via Ion-Selective Pores Maintaining Global Bilayer Integrity}},
url = {{http://dx.doi.org/10.1021/acs.langmuir.2c02487}},
doi = {{10.1021/acs.langmuir.2c02487}},
volume = {{38}},
year = {{2022}},
}