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Quantitative proteomic analysis identifies proteins and pathways related to neuronal development in differentiated SH-SY5Y neuroblastoma cells

Murillo, Jimmy Rodriguez; Goto-Silva, Livia; Sánchez, Aniel LU ; Nogueira, Fábio C.S.; Domont, Gilberto B. and Junqueira, Magno (2017) In EuPA Open Proteomics 16. p.1-11
Abstract

SH-SY5Y neuroblastoma cells are susceptible to differentiation using retinoic acid (RA) and brain-derived neurotrophic factor (BDNF), providing a model of neuronal differentiation. We compared SH-SY5Y cells proteome before and after RA/BDNF treatment using iTRAQ and phosphopeptide enrichment strategies. We identified 5587 proteins, 366 of them with differential abundance. Differentiated cells expressed proteins related to neuronal development, and, undifferentiated cells expressed proteins involved in cell proliferation. Interactive network covered focal adhesion, cytoskeleton dynamics and neurodegenerative diseases processes and regulation of mitogen-activated protein kinase-related signaling pathways; key proteins involved in those... (More)

SH-SY5Y neuroblastoma cells are susceptible to differentiation using retinoic acid (RA) and brain-derived neurotrophic factor (BDNF), providing a model of neuronal differentiation. We compared SH-SY5Y cells proteome before and after RA/BDNF treatment using iTRAQ and phosphopeptide enrichment strategies. We identified 5587 proteins, 366 of them with differential abundance. Differentiated cells expressed proteins related to neuronal development, and, undifferentiated cells expressed proteins involved in cell proliferation. Interactive network covered focal adhesion, cytoskeleton dynamics and neurodegenerative diseases processes and regulation of mitogen-activated protein kinase-related signaling pathways; key proteins involved in those processes might be explored as markers for neuronal differentiation.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
iTRAQ-based proteomics, Neuronal differentiation, Phosphoproteomics, SH-SY5Y cells
in
EuPA Open Proteomics
volume
16
pages
11 pages
publisher
Elsevier
external identifiers
  • scopus:85023609384
ISSN
2212-9685
DOI
10.1016/j.euprot.2017.06.001
language
English
LU publication?
yes
id
8ddd43a1-f407-4ff2-8343-799155ece914
date added to LUP
2017-07-27 09:38:53
date last changed
2018-01-07 12:13:19
@article{8ddd43a1-f407-4ff2-8343-799155ece914,
  abstract     = {<p>SH-SY5Y neuroblastoma cells are susceptible to differentiation using retinoic acid (RA) and brain-derived neurotrophic factor (BDNF), providing a model of neuronal differentiation. We compared SH-SY5Y cells proteome before and after RA/BDNF treatment using iTRAQ and phosphopeptide enrichment strategies. We identified 5587 proteins, 366 of them with differential abundance. Differentiated cells expressed proteins related to neuronal development, and, undifferentiated cells expressed proteins involved in cell proliferation. Interactive network covered focal adhesion, cytoskeleton dynamics and neurodegenerative diseases processes and regulation of mitogen-activated protein kinase-related signaling pathways; key proteins involved in those processes might be explored as markers for neuronal differentiation.</p>},
  author       = {Murillo, Jimmy Rodriguez and Goto-Silva, Livia and Sánchez, Aniel and Nogueira, Fábio C.S. and Domont, Gilberto B. and Junqueira, Magno},
  issn         = {2212-9685},
  keyword      = {iTRAQ-based proteomics,Neuronal differentiation,Phosphoproteomics,SH-SY5Y cells},
  language     = {eng},
  month        = {09},
  pages        = {1--11},
  publisher    = {Elsevier},
  series       = {EuPA Open Proteomics},
  title        = {Quantitative proteomic analysis identifies proteins and pathways related to neuronal development in differentiated SH-SY5Y neuroblastoma cells},
  url          = {http://dx.doi.org/10.1016/j.euprot.2017.06.001},
  volume       = {16},
  year         = {2017},
}