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Insulin release by glucagon and secretin : studies with secretin-glucagon hybrids

Kofod, H. ; Andreu, D. ; Thams, P. ; Merrifield, R. B. ; Hedeskov, C. J. ; Hansen, B. and Lernmark, A. LU orcid (1988) In American Journal of Physiology - Endocrinology and Metabolism 254(4). p.454-458
Abstract

Secretin and glucagon potentiate glucose-induced insulin release. We have compared the effects of secretin and glucagon with that of four hybrid molecules of the two hormones on insulin release and formation of cyclic AMP (cAMP) in isolated mouse pancreatic islets. All six peptides potentiated the release of insulin at 10mM D-glucose, and their effects were indistinguishable with respect to the dynamics of release, dose-response relationship, and glucose dependency. However, measurements of cAMP accumulation in the presence of the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (10-4M) showed that the fold increase compared with glucose alone had the following ranking order: secretin = [Tyr10,... (More)

Secretin and glucagon potentiate glucose-induced insulin release. We have compared the effects of secretin and glucagon with that of four hybrid molecules of the two hormones on insulin release and formation of cyclic AMP (cAMP) in isolated mouse pancreatic islets. All six peptides potentiated the release of insulin at 10mM D-glucose, and their effects were indistinguishable with respect to the dynamics of release, dose-response relationship, and glucose dependency. However, measurements of cAMP accumulation in the presence of the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (10-4M) showed that the fold increase compared with glucose alone had the following ranking order: secretin = [Tyr10, Tyr13]-secretin 1.6<[Tyr10, Tyr13, Trp25]secretin 1.8<glucagon 1.9<[Asp3, Glu9, Arg12]glucagon 2.3 = [Asp3, Glu9]glucagon. These results suggest that despite similar potentiating effects of secretin and glucagon on glucose-induced insulin release, their modes of action may be different.

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author
; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
in
American Journal of Physiology - Endocrinology and Metabolism
volume
254
issue
4
pages
454 - 458
publisher
American Physiological Society
external identifiers
  • scopus:0023937040
  • pmid:2833112
ISSN
0193-1849
DOI
10.1152/ajpendo.1988.254.4.E454
language
English
LU publication?
no
id
8e36990b-0914-4ba5-b372-9f0263ebd9f9
date added to LUP
2019-09-11 10:03:12
date last changed
2024-03-13 08:06:14
@article{8e36990b-0914-4ba5-b372-9f0263ebd9f9,
  abstract     = {{<p>Secretin and glucagon potentiate glucose-induced insulin release. We have compared the effects of secretin and glucagon with that of four hybrid molecules of the two hormones on insulin release and formation of cyclic AMP (cAMP) in isolated mouse pancreatic islets. All six peptides potentiated the release of insulin at 10mM D-glucose, and their effects were indistinguishable with respect to the dynamics of release, dose-response relationship, and glucose dependency. However, measurements of cAMP accumulation in the presence of the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (10<sup>-4</sup>M) showed that the fold increase compared with glucose alone had the following ranking order: secretin = [Tyr<sup>10</sup>, Tyr<sup>13</sup>]-secretin 1.6&lt;[Tyr<sup>10</sup>, Tyr<sup>13</sup>, Trp<sup>25</sup>]secretin 1.8&lt;glucagon 1.9&lt;[Asp<sup>3</sup>, Glu<sup>9</sup>, Arg<sup>12</sup>]glucagon 2.3 = [Asp<sup>3</sup>, Glu<sup>9</sup>]glucagon. These results suggest that despite similar potentiating effects of secretin and glucagon on glucose-induced insulin release, their modes of action may be different.</p>}},
  author       = {{Kofod, H. and Andreu, D. and Thams, P. and Merrifield, R. B. and Hedeskov, C. J. and Hansen, B. and Lernmark, A.}},
  issn         = {{0193-1849}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{4}},
  pages        = {{454--458}},
  publisher    = {{American Physiological Society}},
  series       = {{American Journal of Physiology - Endocrinology and Metabolism}},
  title        = {{Insulin release by glucagon and secretin : studies with secretin-glucagon hybrids}},
  url          = {{http://dx.doi.org/10.1152/ajpendo.1988.254.4.E454}},
  doi          = {{10.1152/ajpendo.1988.254.4.E454}},
  volume       = {{254}},
  year         = {{1988}},
}