A comparison between batch and fed-batch simultaneous saccharification and fermentation of steam pretreated spruce
(2005) In Enzyme and Microbial Technology 37(2). p.195-204- Abstract
- In order to improve the process economy it is important to use as high dry matter content as possible in simultaneous saccharification and fermentation (SSF). However, too high dry matter content often gives rise to severe inhibition of the yeast metabolism, due to the increased levels of toxic compounds. The aim of the present work was to increase the fibrous content in SSF of steam pretreated spruce to 10% by adapting the yeast to the inhibitory substrate and by using a fed-batch process. Both batch and fed-batch approaches were evaluated. The fed-batch experiments were started with a batch fermentation containing 6% dry matter. Fibrous slurry from the pretreatment was then added four times during the first 24 h giving a final dry matter... (More)
- In order to improve the process economy it is important to use as high dry matter content as possible in simultaneous saccharification and fermentation (SSF). However, too high dry matter content often gives rise to severe inhibition of the yeast metabolism, due to the increased levels of toxic compounds. The aim of the present work was to increase the fibrous content in SSF of steam pretreated spruce to 10% by adapting the yeast to the inhibitory substrate and by using a fed-batch process. Both batch and fed-batch approaches were evaluated. The fed-batch experiments were started with a batch fermentation containing 6% dry matter. Fibrous slurry from the pretreatment was then added four times during the first 24 h giving a final dry matter content corresponding to 10%. The yeast used in the fermentation was produced aerobically on the hemicellulose hydrolysate obtained from the pretreatment. SSF batch and fed-batch experiments with a cell mass concentration of 2, 3 and 5 g/L were carried out. When adapted yeast was used, the available hexoses were completely converted within 72 h and the final ethanol concentrations reached 40-44 g/L. No major differences in performance between batch and fed-batch were seen, but the ethanol productivity during the first 24 h was higher in the fed-batch SSF experiments, particularly during the experiments with a cell mass concentration of 2 and 3 g/L. (c) 2005 Elsevier Inc. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/151697
- author
- Rudolf, Andreas LU ; Alkasrawi, Malek LU ; Zacchi, Guido LU and Lidén, Gunnar LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Enzyme and Microbial Technology
- volume
- 37
- issue
- 2
- pages
- 195 - 204
- publisher
- Elsevier
- external identifiers
-
- wos:000229500800005
- scopus:18944392490
- ISSN
- 0141-0229
- DOI
- 10.1016/j.enzmictec.2005.02.013
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Chemical Engineering (011001014), Analytical Chemistry (S/LTH) (011001004)
- id
- 8e47c110-b011-40b6-9127-af2edf1ab7eb (old id 151697)
- date added to LUP
- 2016-04-01 11:41:09
- date last changed
- 2025-04-04 14:05:04
@article{8e47c110-b011-40b6-9127-af2edf1ab7eb,
abstract = {{In order to improve the process economy it is important to use as high dry matter content as possible in simultaneous saccharification and fermentation (SSF). However, too high dry matter content often gives rise to severe inhibition of the yeast metabolism, due to the increased levels of toxic compounds. The aim of the present work was to increase the fibrous content in SSF of steam pretreated spruce to 10% by adapting the yeast to the inhibitory substrate and by using a fed-batch process. Both batch and fed-batch approaches were evaluated. The fed-batch experiments were started with a batch fermentation containing 6% dry matter. Fibrous slurry from the pretreatment was then added four times during the first 24 h giving a final dry matter content corresponding to 10%. The yeast used in the fermentation was produced aerobically on the hemicellulose hydrolysate obtained from the pretreatment. SSF batch and fed-batch experiments with a cell mass concentration of 2, 3 and 5 g/L were carried out. When adapted yeast was used, the available hexoses were completely converted within 72 h and the final ethanol concentrations reached 40-44 g/L. No major differences in performance between batch and fed-batch were seen, but the ethanol productivity during the first 24 h was higher in the fed-batch SSF experiments, particularly during the experiments with a cell mass concentration of 2 and 3 g/L. (c) 2005 Elsevier Inc. All rights reserved.}},
author = {{Rudolf, Andreas and Alkasrawi, Malek and Zacchi, Guido and Lidén, Gunnar}},
issn = {{0141-0229}},
language = {{eng}},
number = {{2}},
pages = {{195--204}},
publisher = {{Elsevier}},
series = {{Enzyme and Microbial Technology}},
title = {{A comparison between batch and fed-batch simultaneous saccharification and fermentation of steam pretreated spruce}},
url = {{http://dx.doi.org/10.1016/j.enzmictec.2005.02.013}},
doi = {{10.1016/j.enzmictec.2005.02.013}},
volume = {{37}},
year = {{2005}},
}