Development of an integrated thermal biosensor for the simultaneous determination of multiple analytes
(1995) In The Analyst 120(1). p.155-160- Abstract
A flow injection thermal microbiosensor was designed for the simultaneous determination of multiple analytes. The biosensor consisted of five thin-film thermistors which were located along a single microchannel. The device was fabricated on a quartz chip by micromachining. The feasibility of employing this system for the detection of two independent enzyme reactions was demonstrated using two different pairs of enzymes, urease-penicillinase and urease-glucose oxidase. The enzymes were immobilized on agarose beads, which were then sequentially packed into distinct regions of the microchannel. Using this method, samples containing urea mixed with penicillin V or with glucose were simultaneously analysed. Linear ranges of up to 20 mmol... (More)
A flow injection thermal microbiosensor was designed for the simultaneous determination of multiple analytes. The biosensor consisted of five thin-film thermistors which were located along a single microchannel. The device was fabricated on a quartz chip by micromachining. The feasibility of employing this system for the detection of two independent enzyme reactions was demonstrated using two different pairs of enzymes, urease-penicillinase and urease-glucose oxidase. The enzymes were immobilized on agarose beads, which were then sequentially packed into distinct regions of the microchannel. Using this method, samples containing urea mixed with penicillin V or with glucose were simultaneously analysed. Linear ranges of up to 20 mmol l-1 urea, 40 mmol l-1 penicillin V and 8 mmol l-1 glucose (saturated with O2) were obtained using a flow rate of 30 μl min-1 and a sample volume of 20 μl. The relative standard deviations for urea and penicillin V assays were 1.13 and 2.42% for the first 100 samples and 1.17 and 2.78% for 200 samples, respectively. The sensor is capable of analysing 25 samples per hour.
(Less)
- author
- Xie, Bin LU ; Mecklenburg, Michael ; Danielsson, Bengt LU ; Öhman, Ove ; Norlin, Peter and Winquist, Fredrik
- organization
- publishing date
- 1995
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Flow injection analysis, Integrated thermistor, Microbiosensor, Multiple analytes, Transducer array
- in
- The Analyst
- volume
- 120
- issue
- 1
- pages
- 6 pages
- publisher
- Royal Society of Chemistry
- external identifiers
-
- scopus:0029168799
- ISSN
- 0003-2654
- DOI
- 10.1039/AN9952000155
- language
- English
- LU publication?
- yes
- id
- 8e75739c-0399-4429-b2af-536060e665ef
- date added to LUP
- 2021-10-27 15:49:28
- date last changed
- 2023-08-30 04:55:23
@article{8e75739c-0399-4429-b2af-536060e665ef,
abstract = {{<p>A flow injection thermal microbiosensor was designed for the simultaneous determination of multiple analytes. The biosensor consisted of five thin-film thermistors which were located along a single microchannel. The device was fabricated on a quartz chip by micromachining. The feasibility of employing this system for the detection of two independent enzyme reactions was demonstrated using two different pairs of enzymes, urease-penicillinase and urease-glucose oxidase. The enzymes were immobilized on agarose beads, which were then sequentially packed into distinct regions of the microchannel. Using this method, samples containing urea mixed with penicillin V or with glucose were simultaneously analysed. Linear ranges of up to 20 mmol l<sup>-1</sup> urea, 40 mmol l<sup>-1</sup> penicillin V and 8 mmol l<sup>-1</sup> glucose (saturated with O<sub>2</sub>) were obtained using a flow rate of 30 μl min<sup>-1</sup> and a sample volume of 20 μl. The relative standard deviations for urea and penicillin V assays were 1.13 and 2.42% for the first 100 samples and 1.17 and 2.78% for 200 samples, respectively. The sensor is capable of analysing 25 samples per hour.</p>}},
author = {{Xie, Bin and Mecklenburg, Michael and Danielsson, Bengt and Öhman, Ove and Norlin, Peter and Winquist, Fredrik}},
issn = {{0003-2654}},
keywords = {{Flow injection analysis; Integrated thermistor; Microbiosensor; Multiple analytes; Transducer array}},
language = {{eng}},
number = {{1}},
pages = {{155--160}},
publisher = {{Royal Society of Chemistry}},
series = {{The Analyst}},
title = {{Development of an integrated thermal biosensor for the simultaneous determination of multiple analytes}},
url = {{http://dx.doi.org/10.1039/AN9952000155}},
doi = {{10.1039/AN9952000155}},
volume = {{120}},
year = {{1995}},
}