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Large-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology Applications

Altincekic, Nadine ; Schlundt, Andreas and Robertson, Angus LU (2021) In Frontiers in Molecular Biosciences 8.
Abstract
The highly infectious disease COVID-19 caused by the Betacoronavirus SARS-CoV-2 poses a severe threat to humanity and demands the redirection of scientific efforts and criteria to organized research projects. The international COVID19-NMR consortium seeks to provide such new approaches by gathering scientific expertise worldwide. In particular, making available viral proteins and RNAs will pave the way to understanding the SARS-CoV-2 molecular components in detail. The research in COVID19-NMR and the resources provided through the consortium are fully disclosed to accelerate access and exploitation. NMR investigations of the viral molecular components are designated to provide the essential basis for further work, including macromolecular... (More)
The highly infectious disease COVID-19 caused by the Betacoronavirus SARS-CoV-2 poses a severe threat to humanity and demands the redirection of scientific efforts and criteria to organized research projects. The international COVID19-NMR consortium seeks to provide such new approaches by gathering scientific expertise worldwide. In particular, making available viral proteins and RNAs will pave the way to understanding the SARS-CoV-2 molecular components in detail. The research in COVID19-NMR and the resources provided through the consortium are fully disclosed to accelerate access and exploitation. NMR investigations of the viral molecular components are designated to provide the essential basis for further work, including macromolecular interaction studies and high-throughput drug screening. Here, we present the extensive catalog of a holistic SARS-CoV-2 protein preparation approach based on the consortium’s collective efforts. We provide protocols for the large-scale production of more than 80% of all SARS-CoV-2 proteins or essential parts of them. Several of the proteins were produced in more than one laboratory, demonstrating the high interoperability between NMR groups worldwide. For the majority of proteins, we can produce isotope-labeled samples of HSQC-grade. Together with several NMR chemical shift assignments made publicly available on covid19-nmr.com, we here provide highly valuable resources for the production of SARS-CoV-2 proteins in isotope-labeled form. (Less)
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organization
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type
Contribution to journal
publication status
published
subject
keywords
accessory proteins, cell-free protein synthesis, COVID-19, intrinsically disordered region, NMR spectroscopy, nonstructural proteins, SARS-CoV-2, structural proteins
in
Frontiers in Molecular Biosciences
volume
8
article number
653148
publisher
Frontiers Media S. A.
external identifiers
  • scopus:85105502063
ISSN
2296-889X
DOI
10.3389/fmolb.2021.653148
language
English
LU publication?
yes
id
8edf8658-405e-4bec-9ece-1e8e82130f74
date added to LUP
2024-01-30 08:58:39
date last changed
2024-02-28 08:16:52
@article{8edf8658-405e-4bec-9ece-1e8e82130f74,
  abstract     = {{The highly infectious disease COVID-19 caused by the Betacoronavirus SARS-CoV-2 poses a severe threat to humanity and demands the redirection of scientific efforts and criteria to organized research projects. The international COVID19-NMR consortium seeks to provide such new approaches by gathering scientific expertise worldwide. In particular, making available viral proteins and RNAs will pave the way to understanding the SARS-CoV-2 molecular components in detail. The research in COVID19-NMR and the resources provided through the consortium are fully disclosed to accelerate access and exploitation. NMR investigations of the viral molecular components are designated to provide the essential basis for further work, including macromolecular interaction studies and high-throughput drug screening. Here, we present the extensive catalog of a holistic SARS-CoV-2 protein preparation approach based on the consortium’s collective efforts. We provide protocols for the large-scale production of more than 80% of all SARS-CoV-2 proteins or essential parts of them. Several of the proteins were produced in more than one laboratory, demonstrating the high interoperability between NMR groups worldwide. For the majority of proteins, we can produce isotope-labeled samples of HSQC-grade. Together with several NMR chemical shift assignments made publicly available on covid19-nmr.com, we here provide highly valuable resources for the production of SARS-CoV-2 proteins in isotope-labeled form.}},
  author       = {{Altincekic, Nadine and Schlundt, Andreas and Robertson, Angus}},
  issn         = {{2296-889X}},
  keywords     = {{accessory proteins; cell-free protein synthesis; COVID-19; intrinsically disordered region; NMR spectroscopy; nonstructural proteins; SARS-CoV-2; structural proteins}},
  language     = {{eng}},
  publisher    = {{Frontiers Media S. A.}},
  series       = {{Frontiers in Molecular Biosciences}},
  title        = {{Large-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology Applications}},
  url          = {{http://dx.doi.org/10.3389/fmolb.2021.653148}},
  doi          = {{10.3389/fmolb.2021.653148}},
  volume       = {{8}},
  year         = {{2021}},
}