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Comparison of In Vivo and Ex Vivo MRI of the Human Hippocampal Formation in the Same Subjects

Wisse, L E M LU orcid ; Adler, D H ; Ittyerah, R ; Pluta, J B ; Robinson, J L ; Schuck, T ; Trojanowski, J Q ; Grossman, M ; Detre, J A and Elliott, M A , et al. (2017) In Cerebral Cortex 27(11). p.5185-5196
Abstract

Multiple techniques for quantification of hippocampal subfields from in vivo MRI have been proposed. Linking in vivo MRI to the underlying histology can help validate and improve these techniques. High-resolution ex vivo MRI can provide an intermediate modality to map information between these very different imaging modalities. This article evaluates the ability to match information between in vivo and ex vivo MRI in the same subjects. We perform rigid and deformable registration on 10 pairs of in vivo (3 T, 0.4 × 0.4 × 2.6 mm3) and ex vivo (9.4 T, 0.2 × 0.2 × 0.2 mm3) scans, and describe differences in MRI appearance between these modalities qualitatively and quantitatively. The feasibility of using this dataset to validate in vivo... (More)

Multiple techniques for quantification of hippocampal subfields from in vivo MRI have been proposed. Linking in vivo MRI to the underlying histology can help validate and improve these techniques. High-resolution ex vivo MRI can provide an intermediate modality to map information between these very different imaging modalities. This article evaluates the ability to match information between in vivo and ex vivo MRI in the same subjects. We perform rigid and deformable registration on 10 pairs of in vivo (3 T, 0.4 × 0.4 × 2.6 mm3) and ex vivo (9.4 T, 0.2 × 0.2 × 0.2 mm3) scans, and describe differences in MRI appearance between these modalities qualitatively and quantitatively. The feasibility of using this dataset to validate in vivo segmentation is evaluated by applying an automatic hippocampal subfield segmentation technique (ASHS) to in vivo scans and comparing SRLM (stratum/radiatum/lacunosum/moleculare) surface to manual tracing on corresponding ex vivo scans (and in 2 cases, histology). Regional increases in thickness are detected in ex vivo scans adjacent to the ventricles and were not related to scanner, resolution differences, or susceptibility artefacts. Satisfactory in vivo/ex vivo registration and subvoxel accuracy of ASHS segmentation of hippocampal SRLM demonstrate the feasibility of using this dataset for validation, and potentially, improvement of in vivo segmentation methods.

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publishing date
type
Contribution to journal
publication status
published
keywords
Aged, Aged, 80 and over, Brain Diseases/diagnostic imaging, Female, Hippocampus/diagnostic imaging, Humans, Image Processing, Computer-Assisted/methods, Magnetic Resonance Imaging/instrumentation, Male, Middle Aged, Organ Size, Pattern Recognition, Automated/methods, Phantoms, Imaging
in
Cerebral Cortex
volume
27
issue
11
pages
5185 - 5196
publisher
Oxford University Press
external identifiers
  • scopus:85034756432
  • pmid:27664967
ISSN
1460-2199
DOI
10.1093/cercor/bhw299
language
English
LU publication?
no
additional info
© The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.
id
8f10e29e-0201-499f-8d72-93bd3d7b4a35
date added to LUP
2024-02-28 14:57:04
date last changed
2024-02-29 07:37:08
@article{8f10e29e-0201-499f-8d72-93bd3d7b4a35,
  abstract     = {{<p>Multiple techniques for quantification of hippocampal subfields from in vivo MRI have been proposed. Linking in vivo MRI to the underlying histology can help validate and improve these techniques. High-resolution ex vivo MRI can provide an intermediate modality to map information between these very different imaging modalities. This article evaluates the ability to match information between in vivo and ex vivo MRI in the same subjects. We perform rigid and deformable registration on 10 pairs of in vivo (3 T, 0.4 × 0.4 × 2.6 mm3) and ex vivo (9.4 T, 0.2 × 0.2 × 0.2 mm3) scans, and describe differences in MRI appearance between these modalities qualitatively and quantitatively. The feasibility of using this dataset to validate in vivo segmentation is evaluated by applying an automatic hippocampal subfield segmentation technique (ASHS) to in vivo scans and comparing SRLM (stratum/radiatum/lacunosum/moleculare) surface to manual tracing on corresponding ex vivo scans (and in 2 cases, histology). Regional increases in thickness are detected in ex vivo scans adjacent to the ventricles and were not related to scanner, resolution differences, or susceptibility artefacts. Satisfactory in vivo/ex vivo registration and subvoxel accuracy of ASHS segmentation of hippocampal SRLM demonstrate the feasibility of using this dataset for validation, and potentially, improvement of in vivo segmentation methods.</p>}},
  author       = {{Wisse, L E M and Adler, D H and Ittyerah, R and Pluta, J B and Robinson, J L and Schuck, T and Trojanowski, J Q and Grossman, M and Detre, J A and Elliott, M A and Toledo, J B and Liu, W and Pickup, S and Das, S R and Wolk, D A and Yushkevich, P A}},
  issn         = {{1460-2199}},
  keywords     = {{Aged; Aged, 80 and over; Brain Diseases/diagnostic imaging; Female; Hippocampus/diagnostic imaging; Humans; Image Processing, Computer-Assisted/methods; Magnetic Resonance Imaging/instrumentation; Male; Middle Aged; Organ Size; Pattern Recognition, Automated/methods; Phantoms, Imaging}},
  language     = {{eng}},
  month        = {{11}},
  number       = {{11}},
  pages        = {{5185--5196}},
  publisher    = {{Oxford University Press}},
  series       = {{Cerebral Cortex}},
  title        = {{Comparison of In Vivo and Ex Vivo MRI of the Human Hippocampal Formation in the Same Subjects}},
  url          = {{http://dx.doi.org/10.1093/cercor/bhw299}},
  doi          = {{10.1093/cercor/bhw299}},
  volume       = {{27}},
  year         = {{2017}},
}