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Pentaisomaltose, an Alternative to DMSO. Engraftment of Cryopreserved Human CD34+ Cells in Immunodeficient NSG Mice

Svalgaard, Jesper Dyrendom; Talkhoncheh, Mehrnaz Safaee LU ; Haastrup, Eva Kannik; Munthe-Fog, Lea; Clausen, Christian; Hansen, Morten Bagge; Andersen, Pernille; Gørløv, Jette Sønderskov; Larsson, Jonas LU and Fischer-Nielsen, Anne (2018) In Cell Transplantation 27(9). p.1407-1412
Abstract

Hematopoietic stem cell transplantation often involves the cryopreservation of stem cell products. Currently, the standard cryoprotective agent (CPA) is dimethyl sulfoxide (DMSO), which is known to cause concentration-related toxicity and side effects when administered to patients. Based on promising in vitro data from our previous study using pentaisomaltose (a 1 kDa subfraction of Dextran 1) as an alternative to DMSO for cryopreservation of hematopoietic progenitor cells (HPCs) from apheresis products, we proceeded to a preclinical model and compared the two CPAs with respect to engraftment of human hematopoietic stem and progenitor cells (HSPCs) in the immunodeficient NSG mouse model. Human HPCs from apheresis products were... (More)

Hematopoietic stem cell transplantation often involves the cryopreservation of stem cell products. Currently, the standard cryoprotective agent (CPA) is dimethyl sulfoxide (DMSO), which is known to cause concentration-related toxicity and side effects when administered to patients. Based on promising in vitro data from our previous study using pentaisomaltose (a 1 kDa subfraction of Dextran 1) as an alternative to DMSO for cryopreservation of hematopoietic progenitor cells (HPCs) from apheresis products, we proceeded to a preclinical model and compared the two CPAs with respect to engraftment of human hematopoietic stem and progenitor cells (HSPCs) in the immunodeficient NSG mouse model. Human HPCs from apheresis products were cryopreserved with either pentaisomaltose or DMSO, and the following outcomes were measured: (1) the post-thaw recovery of cryopreserved cells and clonogenic potential of CD34+ cells and (2) hematopoietic engraftment in NSG mice. We found that recovery and colony-forming cells data were comparable between pentaisomaltose and DMSO. The engraftment data revealed comparable human CD45+ levels in peripheral blood at 8 weeks and bone marrow at 16 weeks post transplantation. Additionally, the frequencies of CD34+CD38low/negative and myeloid/lymphoid cells in the bone marrow were comparable. We here demonstrated that long-term engrafting HSPCs were well preserved in pentaisomaltose and comparable to cells cryopreserved with DMSO. Although a clinical trial is necessary to translate these results into human use, the present data represent an important step toward the replacement of DMSO with a non-toxic alternative.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
CD34 cells, cryopreservation, cryoprotective agent, DMSO, hematopoietic progenitor cells, pentaisomaltose
in
Cell Transplantation
volume
27
issue
9
pages
6 pages
publisher
Cognizant Communication Corporation
external identifiers
  • scopus:85053913407
ISSN
0963-6897
DOI
10.1177/0963689718786226
language
English
LU publication?
yes
id
8f50abfa-e652-4ec2-b663-f9efbbba421c
date added to LUP
2018-10-12 14:05:57
date last changed
2019-03-26 17:01:36
@article{8f50abfa-e652-4ec2-b663-f9efbbba421c,
  abstract     = {<p>Hematopoietic stem cell transplantation often involves the cryopreservation of stem cell products. Currently, the standard cryoprotective agent (CPA) is dimethyl sulfoxide (DMSO), which is known to cause concentration-related toxicity and side effects when administered to patients. Based on promising in vitro data from our previous study using pentaisomaltose (a 1 kDa subfraction of Dextran 1) as an alternative to DMSO for cryopreservation of hematopoietic progenitor cells (HPCs) from apheresis products, we proceeded to a preclinical model and compared the two CPAs with respect to engraftment of human hematopoietic stem and progenitor cells (HSPCs) in the immunodeficient NSG mouse model. Human HPCs from apheresis products were cryopreserved with either pentaisomaltose or DMSO, and the following outcomes were measured: (1) the post-thaw recovery of cryopreserved cells and clonogenic potential of CD34<sup>+</sup> cells and (2) hematopoietic engraftment in NSG mice. We found that recovery and colony-forming cells data were comparable between pentaisomaltose and DMSO. The engraftment data revealed comparable human CD45<sup>+</sup> levels in peripheral blood at 8 weeks and bone marrow at 16 weeks post transplantation. Additionally, the frequencies of CD34<sup>+</sup>CD38<sup>low/negative</sup> and myeloid/lymphoid cells in the bone marrow were comparable. We here demonstrated that long-term engrafting HSPCs were well preserved in pentaisomaltose and comparable to cells cryopreserved with DMSO. Although a clinical trial is necessary to translate these results into human use, the present data represent an important step toward the replacement of DMSO with a non-toxic alternative.</p>},
  author       = {Svalgaard, Jesper Dyrendom and Talkhoncheh, Mehrnaz Safaee and Haastrup, Eva Kannik and Munthe-Fog, Lea and Clausen, Christian and Hansen, Morten Bagge and Andersen, Pernille and Gørløv, Jette Sønderskov and Larsson, Jonas and Fischer-Nielsen, Anne},
  issn         = {0963-6897},
  keyword      = {CD34 cells,cryopreservation,cryoprotective agent,DMSO,hematopoietic progenitor cells,pentaisomaltose},
  language     = {eng},
  number       = {9},
  pages        = {1407--1412},
  publisher    = {Cognizant Communication Corporation},
  series       = {Cell Transplantation},
  title        = {Pentaisomaltose, an Alternative to DMSO. Engraftment of Cryopreserved Human CD34<sup>+</sup> Cells in Immunodeficient NSG Mice},
  url          = {http://dx.doi.org/10.1177/0963689718786226},
  volume       = {27},
  year         = {2018},
}