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Re-evaluation of the impact of BUD21 deletion on xylose utilization by Saccharomyces cerevisiae

Narayanan, Venkatachalam LU ; Sandström, Anders G. LU and Gorwa-Grauslund, Marie F. LU (2023) In Metabolic Engineering Communications 16.
Abstract

Various rational metabolic engineering and random approaches have been applied to introduce and improve xylose utilization and ethanol productivity by Saccharomyces cerevisiae. Among them, the BUD21 gene was identified as an interesting candidate for enhancing xylose consumption as its deletion appeared to be sufficient to improve growth, substrate utilization and ethanol productivity on xylose, even in a laboratory strain lacking a heterologous xylose pathway. The present study aimed at studying the influence of BUD21 deletion in recombinant strains carrying heterologous oxido-reductive xylose utilization pathway. The positive effect of BUD21 gene deletion on aerobic growth and xylose utilization could not be confirmed in two... (More)

Various rational metabolic engineering and random approaches have been applied to introduce and improve xylose utilization and ethanol productivity by Saccharomyces cerevisiae. Among them, the BUD21 gene was identified as an interesting candidate for enhancing xylose consumption as its deletion appeared to be sufficient to improve growth, substrate utilization and ethanol productivity on xylose, even in a laboratory strain lacking a heterologous xylose pathway. The present study aimed at studying the influence of BUD21 deletion in recombinant strains carrying heterologous oxido-reductive xylose utilization pathway. The positive effect of BUD21 gene deletion on aerobic growth and xylose utilization could not be confirmed in two non-engineered laboratory strains (BY4741 and CEN.PK 113-7D) that were grown in YP rich medium with 20 g/L xylose as sole carbon source, despite the fact that effective deletion of BUD21 gene was confirmed using both genotypic (colony PCR) and phenotypic (heat sensitive phenotype of the BUD21 deletion mutant) control experiments. Therefore, the effect of BUD21 deletion on xylose fermentation might be strain- or medium-dependent.

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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
BUD21, BY4741, CEN.PK 113-7D, Saccharomyces cerevisiae, Xylose, YPD vs. Defined medium
in
Metabolic Engineering Communications
volume
16
article number
e00218
publisher
Elsevier
external identifiers
  • pmid:37398920
  • scopus:85146897474
ISSN
2214-0301
DOI
10.1016/j.mec.2023.e00218
language
English
LU publication?
yes
id
8fdc1272-43ec-4d8a-a3b3-36ae81663ce0
date added to LUP
2023-02-09 15:48:54
date last changed
2024-04-16 07:24:01
@article{8fdc1272-43ec-4d8a-a3b3-36ae81663ce0,
  abstract     = {{<p>Various rational metabolic engineering and random approaches have been applied to introduce and improve xylose utilization and ethanol productivity by Saccharomyces cerevisiae. Among them, the BUD21 gene was identified as an interesting candidate for enhancing xylose consumption as its deletion appeared to be sufficient to improve growth, substrate utilization and ethanol productivity on xylose, even in a laboratory strain lacking a heterologous xylose pathway. The present study aimed at studying the influence of BUD21 deletion in recombinant strains carrying heterologous oxido-reductive xylose utilization pathway. The positive effect of BUD21 gene deletion on aerobic growth and xylose utilization could not be confirmed in two non-engineered laboratory strains (BY4741 and CEN.PK 113-7D) that were grown in YP rich medium with 20 g/L xylose as sole carbon source, despite the fact that effective deletion of BUD21 gene was confirmed using both genotypic (colony PCR) and phenotypic (heat sensitive phenotype of the BUD21 deletion mutant) control experiments. Therefore, the effect of BUD21 deletion on xylose fermentation might be strain- or medium-dependent.</p>}},
  author       = {{Narayanan, Venkatachalam and Sandström, Anders G. and Gorwa-Grauslund, Marie F.}},
  issn         = {{2214-0301}},
  keywords     = {{BUD21; BY4741; CEN.PK 113-7D; Saccharomyces cerevisiae; Xylose; YPD vs. Defined medium}},
  language     = {{eng}},
  publisher    = {{Elsevier}},
  series       = {{Metabolic Engineering Communications}},
  title        = {{Re-evaluation of the impact of <i>BUD21</i> deletion on xylose utilization by <i>Saccharomyces cerevisiae</i>}},
  url          = {{http://dx.doi.org/10.1016/j.mec.2023.e00218}},
  doi          = {{10.1016/j.mec.2023.e00218}},
  volume       = {{16}},
  year         = {{2023}},
}