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Oxidative DNA damage in human sperm influences time to pregnancy

Loft, S ; Kold-Jensen, T ; Hjollun, NH ; Giwercman, Aleksander LU ; Gyllemborg, J ; Ernst, E ; Olsen, J ; Scheike, T ; Poulsen, HE and Bonde, JP (2003) In Human Reproduction 18(6). p.1265-1272
Abstract
BACKGROUND: Oxidative stress and related DNA damage in human sperm may be important for fecundity and pregnancy outcome. METHODS: We studied the level of oxidative DNA damage in terms of 7-hydro-8-oxo-2'-deoxyguanosine (8-oxodG) in sperm DNA among 225 first-pregnancy planners. Over the six menstrual cycle follow-up time, after cessation of contraception, 135 pregnancies were conceived. RESULTS: The likelihood of pregnancy occurring in a single menstrual cycle was inversely associated with the 8-oxodG level (P < 0.01). The odds ratio of pregnancy in each of the first three or all six follow-up menstrual cycles was 0.42 (0.23-0.78; 95% CI) and 0.61 (0.36-0.91) per unit increase in the log 8-oxodG/100 000 dG ratio after adjustment for... (More)
BACKGROUND: Oxidative stress and related DNA damage in human sperm may be important for fecundity and pregnancy outcome. METHODS: We studied the level of oxidative DNA damage in terms of 7-hydro-8-oxo-2'-deoxyguanosine (8-oxodG) in sperm DNA among 225 first-pregnancy planners. Over the six menstrual cycle follow-up time, after cessation of contraception, 135 pregnancies were conceived. RESULTS: The likelihood of pregnancy occurring in a single menstrual cycle was inversely associated with the 8-oxodG level (P < 0.01). The odds ratio of pregnancy in each of the first three or all six follow-up menstrual cycles was 0.42 (0.23-0.78; 95% CI) and 0.61 (0.36-0.91) per unit increase in the log 8-oxodG/100 000 dG ratio after adjustment for potential confounders, (including sperm concentration) respectively. The intra-individual coefficient of variation of 8-oxodG in 2-6 monthly repeated sperm samples from 116 men was 19% for the 8-oxodG/dG ratio, whereas the inter-individual coefficient of variation was 49%. The 8-oxodG level was not significantly associated with smoking, consumption of alcohol or caffeine, exposure to welding fumes or the plasma levels of sex hormones. CONCLUSIONS: The data suggest that oxidative damage to sperm DNA influences fecundity and the level of damage is relatively constant within an individual and not influenced by smoking. (Less)
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author
; ; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
time to pregnancy, smoking, oxidative DNA damage, 8-oxodeoxyguanosine, male fecundity
in
Human Reproduction
volume
18
issue
6
pages
1265 - 1272
publisher
Oxford University Press
external identifiers
  • pmid:12773457
  • wos:000183421100022
  • scopus:10744222306
ISSN
0268-1161
DOI
10.1093/humrep/deg202
language
English
LU publication?
yes
id
f8305e49-ca1e-4fed-b943-6416731a1684 (old id 900321)
alternative location
http://humrep.oxfordjournals.org/cgi/reprint/18/6/1265
date added to LUP
2016-04-01 12:14:05
date last changed
2022-05-19 03:01:30
@article{f8305e49-ca1e-4fed-b943-6416731a1684,
  abstract     = {{BACKGROUND: Oxidative stress and related DNA damage in human sperm may be important for fecundity and pregnancy outcome. METHODS: We studied the level of oxidative DNA damage in terms of 7-hydro-8-oxo-2'-deoxyguanosine (8-oxodG) in sperm DNA among 225 first-pregnancy planners. Over the six menstrual cycle follow-up time, after cessation of contraception, 135 pregnancies were conceived. RESULTS: The likelihood of pregnancy occurring in a single menstrual cycle was inversely associated with the 8-oxodG level (P &lt; 0.01). The odds ratio of pregnancy in each of the first three or all six follow-up menstrual cycles was 0.42 (0.23-0.78; 95% CI) and 0.61 (0.36-0.91) per unit increase in the log 8-oxodG/100 000 dG ratio after adjustment for potential confounders, (including sperm concentration) respectively. The intra-individual coefficient of variation of 8-oxodG in 2-6 monthly repeated sperm samples from 116 men was 19% for the 8-oxodG/dG ratio, whereas the inter-individual coefficient of variation was 49%. The 8-oxodG level was not significantly associated with smoking, consumption of alcohol or caffeine, exposure to welding fumes or the plasma levels of sex hormones. CONCLUSIONS: The data suggest that oxidative damage to sperm DNA influences fecundity and the level of damage is relatively constant within an individual and not influenced by smoking.}},
  author       = {{Loft, S and Kold-Jensen, T and Hjollun, NH and Giwercman, Aleksander and Gyllemborg, J and Ernst, E and Olsen, J and Scheike, T and Poulsen, HE and Bonde, JP}},
  issn         = {{0268-1161}},
  keywords     = {{time to pregnancy; smoking; oxidative DNA damage; 8-oxodeoxyguanosine; male fecundity}},
  language     = {{eng}},
  number       = {{6}},
  pages        = {{1265--1272}},
  publisher    = {{Oxford University Press}},
  series       = {{Human Reproduction}},
  title        = {{Oxidative DNA damage in human sperm influences time to pregnancy}},
  url          = {{http://dx.doi.org/10.1093/humrep/deg202}},
  doi          = {{10.1093/humrep/deg202}},
  volume       = {{18}},
  year         = {{2003}},
}