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Oxidative DNA damage in human sperm influences time to pregnancy

Loft, S; Kold-Jensen, T; Hjollun, NH; Giwercman, Aleksander LU ; Gyllemborg, J; Ernst, E; Olsen, J; Scheike, T; Poulsen, HE and Bonde, JP (2003) In Human Reproduction 18(6). p.1265-1272
Abstract
BACKGROUND: Oxidative stress and related DNA damage in human sperm may be important for fecundity and pregnancy outcome. METHODS: We studied the level of oxidative DNA damage in terms of 7-hydro-8-oxo-2'-deoxyguanosine (8-oxodG) in sperm DNA among 225 first-pregnancy planners. Over the six menstrual cycle follow-up time, after cessation of contraception, 135 pregnancies were conceived. RESULTS: The likelihood of pregnancy occurring in a single menstrual cycle was inversely associated with the 8-oxodG level (P < 0.01). The odds ratio of pregnancy in each of the first three or all six follow-up menstrual cycles was 0.42 (0.23-0.78; 95% CI) and 0.61 (0.36-0.91) per unit increase in the log 8-oxodG/100 000 dG ratio after adjustment for... (More)
BACKGROUND: Oxidative stress and related DNA damage in human sperm may be important for fecundity and pregnancy outcome. METHODS: We studied the level of oxidative DNA damage in terms of 7-hydro-8-oxo-2'-deoxyguanosine (8-oxodG) in sperm DNA among 225 first-pregnancy planners. Over the six menstrual cycle follow-up time, after cessation of contraception, 135 pregnancies were conceived. RESULTS: The likelihood of pregnancy occurring in a single menstrual cycle was inversely associated with the 8-oxodG level (P < 0.01). The odds ratio of pregnancy in each of the first three or all six follow-up menstrual cycles was 0.42 (0.23-0.78; 95% CI) and 0.61 (0.36-0.91) per unit increase in the log 8-oxodG/100 000 dG ratio after adjustment for potential confounders, (including sperm concentration) respectively. The intra-individual coefficient of variation of 8-oxodG in 2-6 monthly repeated sperm samples from 116 men was 19% for the 8-oxodG/dG ratio, whereas the inter-individual coefficient of variation was 49%. The 8-oxodG level was not significantly associated with smoking, consumption of alcohol or caffeine, exposure to welding fumes or the plasma levels of sex hormones. CONCLUSIONS: The data suggest that oxidative damage to sperm DNA influences fecundity and the level of damage is relatively constant within an individual and not influenced by smoking. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
time to pregnancy, smoking, oxidative DNA damage, 8-oxodeoxyguanosine, male fecundity
in
Human Reproduction
volume
18
issue
6
pages
1265 - 1272
publisher
Oxford University Press
external identifiers
  • pmid:12773457
  • wos:000183421100022
  • scopus:10744222306
ISSN
0268-1161
DOI
10.1093/humrep/deg202
language
English
LU publication?
yes
id
f8305e49-ca1e-4fed-b943-6416731a1684 (old id 900321)
alternative location
http://humrep.oxfordjournals.org/cgi/reprint/18/6/1265
date added to LUP
2008-01-14 21:01:22
date last changed
2018-01-28 03:30:17
@article{f8305e49-ca1e-4fed-b943-6416731a1684,
  abstract     = {BACKGROUND: Oxidative stress and related DNA damage in human sperm may be important for fecundity and pregnancy outcome. METHODS: We studied the level of oxidative DNA damage in terms of 7-hydro-8-oxo-2'-deoxyguanosine (8-oxodG) in sperm DNA among 225 first-pregnancy planners. Over the six menstrual cycle follow-up time, after cessation of contraception, 135 pregnancies were conceived. RESULTS: The likelihood of pregnancy occurring in a single menstrual cycle was inversely associated with the 8-oxodG level (P &lt; 0.01). The odds ratio of pregnancy in each of the first three or all six follow-up menstrual cycles was 0.42 (0.23-0.78; 95% CI) and 0.61 (0.36-0.91) per unit increase in the log 8-oxodG/100 000 dG ratio after adjustment for potential confounders, (including sperm concentration) respectively. The intra-individual coefficient of variation of 8-oxodG in 2-6 monthly repeated sperm samples from 116 men was 19% for the 8-oxodG/dG ratio, whereas the inter-individual coefficient of variation was 49%. The 8-oxodG level was not significantly associated with smoking, consumption of alcohol or caffeine, exposure to welding fumes or the plasma levels of sex hormones. CONCLUSIONS: The data suggest that oxidative damage to sperm DNA influences fecundity and the level of damage is relatively constant within an individual and not influenced by smoking.},
  author       = {Loft, S and Kold-Jensen, T and Hjollun, NH and Giwercman, Aleksander and Gyllemborg, J and Ernst, E and Olsen, J and Scheike, T and Poulsen, HE and Bonde, JP},
  issn         = {0268-1161},
  keyword      = {time to pregnancy,smoking,oxidative DNA damage,8-oxodeoxyguanosine,male fecundity},
  language     = {eng},
  number       = {6},
  pages        = {1265--1272},
  publisher    = {Oxford University Press},
  series       = {Human Reproduction},
  title        = {Oxidative DNA damage in human sperm influences time to pregnancy},
  url          = {http://dx.doi.org/10.1093/humrep/deg202},
  volume       = {18},
  year         = {2003},
}