Evidence that Hy-RBCs express weak Jo(a) antigen
(2004) In Transfusion 44(2). p.170-172- Abstract
- BACKGROUND: RBCs of the Hy- phenotype have, in the past, been typed as Gy(a+(w)), Hy-, Jo(a-), and RBCs with the Jo(a-) phenotype type Gy(a+), Hy-(w), and Jo(a-). Anti-Hy and anti-Jo(a) are difficult to identify mainly because appropriate reagent R6Cs are poorly characterized. Historically, anti-Jo(a) has not reacted with RBCs with either phenotype. This report describes a case of an anti-Jo(a) that shows Hy- RBCs express some Jo(a) antigen, albeit weakly. CASE REPORT: Anti-Jo(a) was identified in a serum sample of a 71-year-old woman. The antibody reacted 1+ to 2+ by the IAT with all untreated and ficin-treated panel RBCs and did not react with Gy(a-) RBCs and Jo(a-) RBCs. Unexpectedly, the serum sample reacted weakly with six of eight... (More)
- BACKGROUND: RBCs of the Hy- phenotype have, in the past, been typed as Gy(a+(w)), Hy-, Jo(a-), and RBCs with the Jo(a-) phenotype type Gy(a+), Hy-(w), and Jo(a-). Anti-Hy and anti-Jo(a) are difficult to identify mainly because appropriate reagent R6Cs are poorly characterized. Historically, anti-Jo(a) has not reacted with RBCs with either phenotype. This report describes a case of an anti-Jo(a) that shows Hy- RBCs express some Jo(a) antigen, albeit weakly. CASE REPORT: Anti-Jo(a) was identified in a serum sample of a 71-year-old woman. The antibody reacted 1+ to 2+ by the IAT with all untreated and ficin-treated panel RBCs and did not react with Gy(a-) RBCs and Jo(a-) RBCs. Unexpectedly, the serum sample reacted weakly with six of eight RBC samples with the Hy- phenotype. The anti_Jo(a) was adsorbed onto and eluted from Hy- RBCs, indicating the presence of weak Jo(a) antigen. The patient's RBCs typed Gy(a+), Hy+, Jo(a-). DNA studies using PCR-RFLP analysis showed the patient to be homozygous for the JO allele, which is consistent with the serologically determined Jo(a-) status. CONCLUSION: The DNA and serologic evidence of this case show that Hy- RBCs may express low levels of Jo(a) antigen, which contradicts previously published data concerning the Jo(a) type of Hy- RBCs. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/906761
- author
- Scofield, TL ; Miller, JP ; Storry, Jill LU ; Rios, M and Reid, ME
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Transfusion
- volume
- 44
- issue
- 2
- pages
- 170 - 172
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000189055900006
- pmid:14962307
- scopus:1242277303
- ISSN
- 1537-2995
- DOI
- 10.1111/j.1537-2995.2004.00627.x
- language
- English
- LU publication?
- yes
- id
- b4e8535d-af60-46f7-ae3f-48854dee998c (old id 906761)
- date added to LUP
- 2016-04-01 17:12:26
- date last changed
- 2022-01-29 01:06:21
@article{b4e8535d-af60-46f7-ae3f-48854dee998c, abstract = {{BACKGROUND: RBCs of the Hy- phenotype have, in the past, been typed as Gy(a+(w)), Hy-, Jo(a-), and RBCs with the Jo(a-) phenotype type Gy(a+), Hy-(w), and Jo(a-). Anti-Hy and anti-Jo(a) are difficult to identify mainly because appropriate reagent R6Cs are poorly characterized. Historically, anti-Jo(a) has not reacted with RBCs with either phenotype. This report describes a case of an anti-Jo(a) that shows Hy- RBCs express some Jo(a) antigen, albeit weakly. CASE REPORT: Anti-Jo(a) was identified in a serum sample of a 71-year-old woman. The antibody reacted 1+ to 2+ by the IAT with all untreated and ficin-treated panel RBCs and did not react with Gy(a-) RBCs and Jo(a-) RBCs. Unexpectedly, the serum sample reacted weakly with six of eight RBC samples with the Hy- phenotype. The anti_Jo(a) was adsorbed onto and eluted from Hy- RBCs, indicating the presence of weak Jo(a) antigen. The patient's RBCs typed Gy(a+), Hy+, Jo(a-). DNA studies using PCR-RFLP analysis showed the patient to be homozygous for the JO allele, which is consistent with the serologically determined Jo(a-) status. CONCLUSION: The DNA and serologic evidence of this case show that Hy- RBCs may express low levels of Jo(a) antigen, which contradicts previously published data concerning the Jo(a) type of Hy- RBCs.}}, author = {{Scofield, TL and Miller, JP and Storry, Jill and Rios, M and Reid, ME}}, issn = {{1537-2995}}, language = {{eng}}, number = {{2}}, pages = {{170--172}}, publisher = {{Wiley-Blackwell}}, series = {{Transfusion}}, title = {{Evidence that Hy-RBCs express weak Jo(a) antigen}}, url = {{http://dx.doi.org/10.1111/j.1537-2995.2004.00627.x}}, doi = {{10.1111/j.1537-2995.2004.00627.x}}, volume = {{44}}, year = {{2004}}, }