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Evidence that Hy-RBCs express weak Jo(a) antigen

Scofield, TL; Miller, JP; Storry, Jill LU ; Rios, M and Reid, ME (2004) In Transfusion 44(2). p.170-172
Abstract
BACKGROUND: RBCs of the Hy- phenotype have, in the past, been typed as Gy(a+(w)), Hy-, Jo(a-), and RBCs with the Jo(a-) phenotype type Gy(a+), Hy-(w), and Jo(a-). Anti-Hy and anti-Jo(a) are difficult to identify mainly because appropriate reagent R6Cs are poorly characterized. Historically, anti-Jo(a) has not reacted with RBCs with either phenotype. This report describes a case of an anti-Jo(a) that shows Hy- RBCs express some Jo(a) antigen, albeit weakly. CASE REPORT: Anti-Jo(a) was identified in a serum sample of a 71-year-old woman. The antibody reacted 1+ to 2+ by the IAT with all untreated and ficin-treated panel RBCs and did not react with Gy(a-) RBCs and Jo(a-) RBCs. Unexpectedly, the serum sample reacted weakly with six of eight... (More)
BACKGROUND: RBCs of the Hy- phenotype have, in the past, been typed as Gy(a+(w)), Hy-, Jo(a-), and RBCs with the Jo(a-) phenotype type Gy(a+), Hy-(w), and Jo(a-). Anti-Hy and anti-Jo(a) are difficult to identify mainly because appropriate reagent R6Cs are poorly characterized. Historically, anti-Jo(a) has not reacted with RBCs with either phenotype. This report describes a case of an anti-Jo(a) that shows Hy- RBCs express some Jo(a) antigen, albeit weakly. CASE REPORT: Anti-Jo(a) was identified in a serum sample of a 71-year-old woman. The antibody reacted 1+ to 2+ by the IAT with all untreated and ficin-treated panel RBCs and did not react with Gy(a-) RBCs and Jo(a-) RBCs. Unexpectedly, the serum sample reacted weakly with six of eight RBC samples with the Hy- phenotype. The anti_Jo(a) was adsorbed onto and eluted from Hy- RBCs, indicating the presence of weak Jo(a) antigen. The patient's RBCs typed Gy(a+), Hy+, Jo(a-). DNA studies using PCR-RFLP analysis showed the patient to be homozygous for the JO allele, which is consistent with the serologically determined Jo(a-) status. CONCLUSION: The DNA and serologic evidence of this case show that Hy- RBCs may express low levels of Jo(a) antigen, which contradicts previously published data concerning the Jo(a) type of Hy- RBCs. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Transfusion
volume
44
issue
2
pages
170 - 172
publisher
Wiley-Blackwell
external identifiers
  • wos:000189055900006
  • pmid:14962307
  • scopus:1242277303
ISSN
1537-2995
DOI
10.1111/j.1537-2995.2004.00627.x
language
English
LU publication?
yes
id
b4e8535d-af60-46f7-ae3f-48854dee998c (old id 906761)
date added to LUP
2008-01-18 12:23:48
date last changed
2017-01-01 07:27:16
@article{b4e8535d-af60-46f7-ae3f-48854dee998c,
  abstract     = {BACKGROUND: RBCs of the Hy- phenotype have, in the past, been typed as Gy(a+(w)), Hy-, Jo(a-), and RBCs with the Jo(a-) phenotype type Gy(a+), Hy-(w), and Jo(a-). Anti-Hy and anti-Jo(a) are difficult to identify mainly because appropriate reagent R6Cs are poorly characterized. Historically, anti-Jo(a) has not reacted with RBCs with either phenotype. This report describes a case of an anti-Jo(a) that shows Hy- RBCs express some Jo(a) antigen, albeit weakly. CASE REPORT: Anti-Jo(a) was identified in a serum sample of a 71-year-old woman. The antibody reacted 1+ to 2+ by the IAT with all untreated and ficin-treated panel RBCs and did not react with Gy(a-) RBCs and Jo(a-) RBCs. Unexpectedly, the serum sample reacted weakly with six of eight RBC samples with the Hy- phenotype. The anti_Jo(a) was adsorbed onto and eluted from Hy- RBCs, indicating the presence of weak Jo(a) antigen. The patient's RBCs typed Gy(a+), Hy+, Jo(a-). DNA studies using PCR-RFLP analysis showed the patient to be homozygous for the JO allele, which is consistent with the serologically determined Jo(a-) status. CONCLUSION: The DNA and serologic evidence of this case show that Hy- RBCs may express low levels of Jo(a) antigen, which contradicts previously published data concerning the Jo(a) type of Hy- RBCs.},
  author       = {Scofield, TL and Miller, JP and Storry, Jill and Rios, M and Reid, ME},
  issn         = {1537-2995},
  language     = {eng},
  number       = {2},
  pages        = {170--172},
  publisher    = {Wiley-Blackwell},
  series       = {Transfusion},
  title        = {Evidence that Hy-RBCs express weak Jo(a) antigen},
  url          = {http://dx.doi.org/10.1111/j.1537-2995.2004.00627.x},
  volume       = {44},
  year         = {2004},
}