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Bienzyme biosensors for glucose, ethanol and putrescine built on oxidase and sweet potato peroxidase

Castillo Leon, Jaime LU ; Gaspar, Szilveszter LU ; Sakharov, I and Csöregi, Elisabeth LU (2003) Seventh World Congress on Biosensors In Biosensors & Bioelectronics 18(5-6). p.705-714
Abstract
Amperometric biosensors for glucose, ethanol, and biogenic amines (putrescine) were constructed using oxidase/peroxidase bienzyme systems. The H<sub>2</sub>O<sub>2</sub> produced by the oxidase in reaction with its substrate is converted into a measurable signal via a novel peroxidase purified from sweet potato peels. All developed biosensors are based on redox hydrogels formed of oxidases (glucose oxidase, alcohol oxidase, or amine oxidase) and the newly purified sweet potato peroxidase (SPP) cross-linked to a redox polymer. The developed electrodes were characterized (sensitivity, stability, and performances in organic medium) and compared with similarly built ones using the 'classical' horseradish peroxidase... (More)
Amperometric biosensors for glucose, ethanol, and biogenic amines (putrescine) were constructed using oxidase/peroxidase bienzyme systems. The H<sub>2</sub>O<sub>2</sub> produced by the oxidase in reaction with its substrate is converted into a measurable signal via a novel peroxidase purified from sweet potato peels. All developed biosensors are based on redox hydrogels formed of oxidases (glucose oxidase, alcohol oxidase, or amine oxidase) and the newly purified sweet potato peroxidase (SPP) cross-linked to a redox polymer. The developed electrodes were characterized (sensitivity, stability, and performances in organic medium) and compared with similarly built ones using the 'classical' horseradish peroxidase (HRP). The SPP-based electrodes displayed higher sensitivity and better detection limit for putrescine than those using HRP and were also shown to retain their activity in organic phase much better than the HPR based ones. The importance of attractive or repulsive electrostatic interactions between the peroxidases and oxidases (determined by their isoelectric points) were found to play an important role in the sensitivity of the obtained sensors (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
amperometric biosensor, bienzyme electrode, redox hydrogel, sweet, potato peroxidase
in
Biosensors & Bioelectronics
volume
18
issue
5-6
pages
10 pages
publisher
Elsevier
conference name
Seventh World Congress on Biosensors
external identifiers
  • wos:000182790500028
  • pmid:12706582
  • scopus:0037403202
ISSN
1873-4235
DOI
10.1016/S0956-5663(03)00011-3
language
English
LU publication?
yes
id
76e40448-c503-474c-a3f5-2723d23bfa46 (old id 907627)
date added to LUP
2008-01-10 18:41:05
date last changed
2018-05-29 11:38:43
@article{76e40448-c503-474c-a3f5-2723d23bfa46,
  abstract     = {Amperometric biosensors for glucose, ethanol, and biogenic amines (putrescine) were constructed using oxidase/peroxidase bienzyme systems. The H&lt;sub&gt;2&lt;/sub&gt;O&lt;sub&gt;2&lt;/sub&gt; produced by the oxidase in reaction with its substrate is converted into a measurable signal via a novel peroxidase purified from sweet potato peels. All developed biosensors are based on redox hydrogels formed of oxidases (glucose oxidase, alcohol oxidase, or amine oxidase) and the newly purified sweet potato peroxidase (SPP) cross-linked to a redox polymer. The developed electrodes were characterized (sensitivity, stability, and performances in organic medium) and compared with similarly built ones using the 'classical' horseradish peroxidase (HRP). The SPP-based electrodes displayed higher sensitivity and better detection limit for putrescine than those using HRP and were also shown to retain their activity in organic phase much better than the HPR based ones. The importance of attractive or repulsive electrostatic interactions between the peroxidases and oxidases (determined by their isoelectric points) were found to play an important role in the sensitivity of the obtained sensors},
  author       = {Castillo Leon, Jaime and Gaspar, Szilveszter and Sakharov, I and Csöregi, Elisabeth},
  issn         = {1873-4235},
  keyword      = {amperometric biosensor,bienzyme electrode,redox hydrogel,sweet,potato peroxidase},
  language     = {eng},
  number       = {5-6},
  pages        = {705--714},
  publisher    = {Elsevier},
  series       = {Biosensors & Bioelectronics},
  title        = {Bienzyme biosensors for glucose, ethanol and putrescine built on oxidase and sweet potato peroxidase},
  url          = {http://dx.doi.org/10.1016/S0956-5663(03)00011-3},
  volume       = {18},
  year         = {2003},
}