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Plant based production of myoglobin - a novel source of the muscle heme-protein

Carlsson, Magnus L.R. ; Kanagarajan, Selvaraju ; Bülow, Leif LU and Zhu, Li Hua (2020) In Scientific Reports 10.
Abstract

Myoglobin is a heme-protein in the muscle of vertebrates with important functions in the oxygenation of tissues and as a regulator in nitric oxide signaling. Myoglobin from many species is also an important nutritional source of bioavailable iron. In this study, we have successfully produced human myoglobin in the leaves of Nicotiana benthamiana by transient expression using a viral vector delivered by Agrobacterium tumefaciens. Analyses confirmed that heme was incorporated and the protein was functional, with observed properties consistent with those of native myoglobins. A relatively high degree of purity could be achieved with low cost methods. The results show the high potential of plants as a production platform for heme proteins,... (More)

Myoglobin is a heme-protein in the muscle of vertebrates with important functions in the oxygenation of tissues and as a regulator in nitric oxide signaling. Myoglobin from many species is also an important nutritional source of bioavailable iron. In this study, we have successfully produced human myoglobin in the leaves of Nicotiana benthamiana by transient expression using a viral vector delivered by Agrobacterium tumefaciens. Analyses confirmed that heme was incorporated and the protein was functional, with observed properties consistent with those of native myoglobins. A relatively high degree of purity could be achieved with low cost methods. The results show the high potential of plants as a production platform for heme proteins, a group of proteins of interest for iron nutrition applications and possible future pharmaceutical development.

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author
organization
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type
Contribution to journal
publication status
published
subject
in
Scientific Reports
volume
10
article number
920
publisher
Nature Publishing Group
external identifiers
  • pmid:31969582
  • scopus:85078165667
ISSN
2045-2322
DOI
10.1038/s41598-020-57565-y
language
English
LU publication?
yes
id
909d45c8-2507-488c-9fa5-ad5f681990ef
date added to LUP
2020-02-04 08:37:04
date last changed
2020-02-12 10:20:26
@article{909d45c8-2507-488c-9fa5-ad5f681990ef,
  abstract     = {<p>Myoglobin is a heme-protein in the muscle of vertebrates with important functions in the oxygenation of tissues and as a regulator in nitric oxide signaling. Myoglobin from many species is also an important nutritional source of bioavailable iron. In this study, we have successfully produced human myoglobin in the leaves of Nicotiana benthamiana by transient expression using a viral vector delivered by Agrobacterium tumefaciens. Analyses confirmed that heme was incorporated and the protein was functional, with observed properties consistent with those of native myoglobins. A relatively high degree of purity could be achieved with low cost methods. The results show the high potential of plants as a production platform for heme proteins, a group of proteins of interest for iron nutrition applications and possible future pharmaceutical development.</p>},
  author       = {Carlsson, Magnus L.R. and Kanagarajan, Selvaraju and Bülow, Leif and Zhu, Li Hua},
  issn         = {2045-2322},
  language     = {eng},
  month        = {01},
  publisher    = {Nature Publishing Group},
  series       = {Scientific Reports},
  title        = {Plant based production of myoglobin - a novel source of the muscle heme-protein},
  url          = {http://dx.doi.org/10.1038/s41598-020-57565-y},
  doi          = {10.1038/s41598-020-57565-y},
  volume       = {10},
  year         = {2020},
}