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Detection of Candida dubliniensis in oropharyngeal samples of Turkish HIV-positive patients

Tekeli, A ; Koyuncu, E ; Dolapci, I ; Guven, G S ; Sahin, G O LU and Uzun, O (2005) In Mycoses 48(3). p.197-201
Abstract

The incidence of Candida dubliniensis in immunocomprimised patients in Turkey has not yet been determined. In this study the presence of C. dubliniensis in oral rinse samples of human immunodeficiency virus (HIV)-positive patients and healthy controls were investigated. Phenotypic tests like inability of growth at 45 degrees C, colony formation on Staib agar, intracellular beta-D-glucosidase activity, carbohydrate assimilation profiles and polymerase chain reaction with species-specific primers (DUBF and DUBR) were carried out for differentiation of C. dubliniensis. Of the 35 patients, four (11.4%) had C.dubliniensis in their oral cavity. Antifungal susceptibility testing of these C. dubliniensis isolates showed fluconazole MICs ranging... (More)

The incidence of Candida dubliniensis in immunocomprimised patients in Turkey has not yet been determined. In this study the presence of C. dubliniensis in oral rinse samples of human immunodeficiency virus (HIV)-positive patients and healthy controls were investigated. Phenotypic tests like inability of growth at 45 degrees C, colony formation on Staib agar, intracellular beta-D-glucosidase activity, carbohydrate assimilation profiles and polymerase chain reaction with species-specific primers (DUBF and DUBR) were carried out for differentiation of C. dubliniensis. Of the 35 patients, four (11.4%) had C.dubliniensis in their oral cavity. Antifungal susceptibility testing of these C. dubliniensis isolates showed fluconazole MICs ranging from <0.06 to 32 microg ml(-1) and amphotericin B from <0.06 to 0.25 microg ml(-1). One isolate was dose-dependently susceptible to fluconazole (32 microg ml(-1)). This study demonstrates C. dubliniensis in HIV-positive patients from Turkey.

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publication status
published
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keywords
Adult, Aged, Amphotericin B/pharmacology, Antifungal Agents/pharmacology, Candida/classification, Candidiasis, Oral/complications, Female, Fluconazole/pharmacology, HIV Infections/complications, Humans, Immunocompromised Host, Male, Microbial Sensitivity Tests, Middle Aged, Mycological Typing Techniques, Oropharynx/microbiology, Polymerase Chain Reaction, Turkey
in
Mycoses
volume
48
issue
3
pages
5 pages
publisher
Wiley-Blackwell
external identifiers
  • pmid:15842337
  • scopus:17844395978
ISSN
0933-7407
DOI
10.1111/j.1439-0507.2005.01125.x
language
English
LU publication?
no
id
90e82d00-fe03-401a-abdd-27e59e5cd007
date added to LUP
2019-06-30 01:02:12
date last changed
2020-01-13 02:09:25
@article{90e82d00-fe03-401a-abdd-27e59e5cd007,
  abstract     = {<p>The incidence of Candida dubliniensis in immunocomprimised patients in Turkey has not yet been determined. In this study the presence of C. dubliniensis in oral rinse samples of human immunodeficiency virus (HIV)-positive patients and healthy controls were investigated. Phenotypic tests like inability of growth at 45 degrees C, colony formation on Staib agar, intracellular beta-D-glucosidase activity, carbohydrate assimilation profiles and polymerase chain reaction with species-specific primers (DUBF and DUBR) were carried out for differentiation of C. dubliniensis. Of the 35 patients, four (11.4%) had C.dubliniensis in their oral cavity. Antifungal susceptibility testing of these C. dubliniensis isolates showed fluconazole MICs ranging from &lt;0.06 to 32 microg ml(-1) and amphotericin B from &lt;0.06 to 0.25 microg ml(-1). One isolate was dose-dependently susceptible to fluconazole (32 microg ml(-1)). This study demonstrates C. dubliniensis in HIV-positive patients from Turkey.</p>},
  author       = {Tekeli, A and Koyuncu, E and Dolapci, I and Guven, G S and Sahin, G O and Uzun, O},
  issn         = {0933-7407},
  language     = {eng},
  number       = {3},
  pages        = {197--201},
  publisher    = {Wiley-Blackwell},
  series       = {Mycoses},
  title        = {Detection of Candida dubliniensis in oropharyngeal samples of Turkish HIV-positive patients},
  url          = {http://dx.doi.org/10.1111/j.1439-0507.2005.01125.x},
  doi          = {10.1111/j.1439-0507.2005.01125.x},
  volume       = {48},
  year         = {2005},
}