Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Quantitative Proteomics Indicate Radical Removal of Non-Small Cell Lung Cancer and Predict Outcome

Bodén, Embla LU ; Andreasson, Jesper LU ; Hirdman, Gabriel LU ; Malmsjö, Malin LU and Lindstedt, Sandra LU (2022) In Biomedicines 10(11).
Abstract

Non-small cell lung cancer (NSCLC) is associated with low survival rates, often due to late diagnosis and lack of personalized medicine. Diagnosing and monitoring NSCLC using blood samples has lately gained interest due to its less invasive nature. In the present study, plasma was collected at three timepoints and analyzed using proximity extension assay technology and quantitative real-time polymerase chain reaction in patients with primary NSCLC stages IA–IIIA undergoing surgery. Results were adjusted for patient demographics, tumor, node, metastasis (TNM) stage, and multiple testing. Major histocompatibility (MHC) class 1 polypeptide-related sequence A/B (MIC-A/B) and tumor necrosis factor ligand superfamily member 6 (FASLG) were... (More)

Non-small cell lung cancer (NSCLC) is associated with low survival rates, often due to late diagnosis and lack of personalized medicine. Diagnosing and monitoring NSCLC using blood samples has lately gained interest due to its less invasive nature. In the present study, plasma was collected at three timepoints and analyzed using proximity extension assay technology and quantitative real-time polymerase chain reaction in patients with primary NSCLC stages IA–IIIA undergoing surgery. Results were adjusted for patient demographics, tumor, node, metastasis (TNM) stage, and multiple testing. Major histocompatibility (MHC) class 1 polypeptide-related sequence A/B (MIC-A/B) and tumor necrosis factor ligand superfamily member 6 (FASLG) were significantly increased post-surgery, suggesting radical removal of cancerous cells. Levels of hepatocyte growth factor (HGF) initially increased postoperatively but were later lowered, potentially indicating radical removal of malignant cells. The levels of FASLG in patients who later died or had a relapse of NSCLC were lower at all three timepoints compared to surviving patients without relapse, indicating that FASLG may be used as a prognostic biomarker. The biomarkers were confirmed using microarray data. In conclusion, quantitative proteomics could be used for NSCLC identification but may also provide information on radical surgical removal of NSCLC and post-surgical prognosis.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
biomarkers, non-small cell lung cancer, proteomics
in
Biomedicines
volume
10
issue
11
article number
2738
publisher
MDPI AG
external identifiers
  • pmid:36359256
  • scopus:85141864150
ISSN
2227-9059
DOI
10.3390/biomedicines10112738
language
English
LU publication?
yes
id
91175cc1-7f7d-4202-83e9-368358b5cd48
date added to LUP
2023-01-04 12:31:38
date last changed
2024-04-04 15:18:35
@article{91175cc1-7f7d-4202-83e9-368358b5cd48,
  abstract     = {{<p>Non-small cell lung cancer (NSCLC) is associated with low survival rates, often due to late diagnosis and lack of personalized medicine. Diagnosing and monitoring NSCLC using blood samples has lately gained interest due to its less invasive nature. In the present study, plasma was collected at three timepoints and analyzed using proximity extension assay technology and quantitative real-time polymerase chain reaction in patients with primary NSCLC stages IA–IIIA undergoing surgery. Results were adjusted for patient demographics, tumor, node, metastasis (TNM) stage, and multiple testing. Major histocompatibility (MHC) class 1 polypeptide-related sequence A/B (MIC-A/B) and tumor necrosis factor ligand superfamily member 6 (FASLG) were significantly increased post-surgery, suggesting radical removal of cancerous cells. Levels of hepatocyte growth factor (HGF) initially increased postoperatively but were later lowered, potentially indicating radical removal of malignant cells. The levels of FASLG in patients who later died or had a relapse of NSCLC were lower at all three timepoints compared to surviving patients without relapse, indicating that FASLG may be used as a prognostic biomarker. The biomarkers were confirmed using microarray data. In conclusion, quantitative proteomics could be used for NSCLC identification but may also provide information on radical surgical removal of NSCLC and post-surgical prognosis.</p>}},
  author       = {{Bodén, Embla and Andreasson, Jesper and Hirdman, Gabriel and Malmsjö, Malin and Lindstedt, Sandra}},
  issn         = {{2227-9059}},
  keywords     = {{biomarkers; non-small cell lung cancer; proteomics}},
  language     = {{eng}},
  number       = {{11}},
  publisher    = {{MDPI AG}},
  series       = {{Biomedicines}},
  title        = {{Quantitative Proteomics Indicate Radical Removal of Non-Small Cell Lung Cancer and Predict Outcome}},
  url          = {{http://dx.doi.org/10.3390/biomedicines10112738}},
  doi          = {{10.3390/biomedicines10112738}},
  volume       = {{10}},
  year         = {{2022}},
}