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Proliferation of human lens epithelial cells (HLE-B3) is inhibited by blocking of voltage-gated calcium channels

Meissner, Anja LU and Noack, Thomas (2008) In Pflügers Archiv 457(1). p.47-59
Abstract

Calcium, as an integral part of a large number of cellular regulatory pathways, is selective in the control of specific cell functions like the start of G1 phase in cell cycle. Cell proliferation has been suggested to depend on increasing intracellular calcium levels. A major regulatory pathway for intracellular calcium is the calcium influx into the cell via voltage-gated calcium channels. T-type and L-type calcium channels are substantially present in human lens epithelial cell (hLEC), and total calcium currents are inhibited by mibefradil. Here, the hypothesis was tested if calcium influx via Ca v channels regulates proliferation in epithelial cells. Cell proliferation was determined by cell culture assays using the L- and... (More)

Calcium, as an integral part of a large number of cellular regulatory pathways, is selective in the control of specific cell functions like the start of G1 phase in cell cycle. Cell proliferation has been suggested to depend on increasing intracellular calcium levels. A major regulatory pathway for intracellular calcium is the calcium influx into the cell via voltage-gated calcium channels. T-type and L-type calcium channels are substantially present in human lens epithelial cell (hLEC), and total calcium currents are inhibited by mibefradil. Here, the hypothesis was tested if calcium influx via Ca v channels regulates proliferation in epithelial cells. Cell proliferation was determined by cell culture assays using the L- and T-type Cav channel blockers mibefradil and verapamil as modulators for calcium influx. Calcium influx was investigated using the Manganese quench technique. Western blot experiments were accomplished under standard conditions using antibodies against MAPK 3. Mibefradil as well as verapamil impaired cell proliferation, but in different concentration ranges. Furthermore, the activation of MAPK 3 was reduced by both antagonists. Calcium influx was also reduced in the presence of both blockers. We conclude that the transmembrane influx of Ca2+ through Cav channels contributes to the regulation of hLEC proliferation, identifying Cav channel blockers as potential therapeutic substances in ocular diseases. © 2008 Springer-Verlag.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Ca influx, Epithelial cell, Protein phosphorylation, T-type calcium channel, Voltage-dependent calcium channel
in
Pflügers Archiv
volume
457
issue
1
pages
13 pages
publisher
Springer
external identifiers
  • scopus:52549096485
ISSN
0031-6768
DOI
10.1007/s00424-008-0514-5
language
English
LU publication?
no
id
912b32b0-2a2f-4642-af46-65837d9e89b4
date added to LUP
2017-05-23 22:26:13
date last changed
2017-07-02 05:02:32
@article{912b32b0-2a2f-4642-af46-65837d9e89b4,
  abstract     = {<p>Calcium, as an integral part of a large number of cellular regulatory pathways, is selective in the control of specific cell functions like the start of G1 phase in cell cycle. Cell proliferation has been suggested to depend on increasing intracellular calcium levels. A major regulatory pathway for intracellular calcium is the calcium influx into the cell via voltage-gated calcium channels. T-type and L-type calcium channels are substantially present in human lens epithelial cell (hLEC), and total calcium currents are inhibited by mibefradil. Here, the hypothesis was tested if calcium influx via Ca <sub>v</sub> channels regulates proliferation in epithelial cells. Cell proliferation was determined by cell culture assays using the L- and T-type Ca<sub>v</sub> channel blockers mibefradil and verapamil as modulators for calcium influx. Calcium influx was investigated using the Manganese quench technique. Western blot experiments were accomplished under standard conditions using antibodies against MAPK 3. Mibefradil as well as verapamil impaired cell proliferation, but in different concentration ranges. Furthermore, the activation of MAPK 3 was reduced by both antagonists. Calcium influx was also reduced in the presence of both blockers. We conclude that the transmembrane influx of Ca<sup>2+</sup> through Ca<sub>v</sub> channels contributes to the regulation of hLEC proliferation, identifying Ca<sub>v</sub> channel blockers as potential therapeutic substances in ocular diseases. © 2008 Springer-Verlag.</p>},
  author       = {Meissner, Anja and Noack, Thomas},
  issn         = {0031-6768},
  keyword      = {Ca influx,Epithelial cell,Protein phosphorylation,T-type calcium channel,Voltage-dependent calcium channel},
  language     = {eng},
  number       = {1},
  pages        = {47--59},
  publisher    = {Springer},
  series       = {Pflügers Archiv},
  title        = {Proliferation of human lens epithelial cells (HLE-B3) is inhibited by blocking of voltage-gated calcium channels},
  url          = {http://dx.doi.org/10.1007/s00424-008-0514-5},
  volume       = {457},
  year         = {2008},
}