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Detection of islet cell autoantibodies in newly diagnosed diabetic patients using insulin-producing Syrian hamster cells.

Matsuba, I. ; Marner, B. ; Nerup, J. LU and Lernmark, A. LU orcid (1987) In Diabetes Research 4(3). p.103-107
Abstract

An insulin-producing cell line, Clone-16, of hamster origin, was characterized for islet hormone production and for reactivity with islet cell surface (ICSA) and islet cell cytoplasmic (ICA) antibodies in sera from children with newly diagnosed insulin-dependent (Type 1) diabetes mellitus (IDDM). The Clone-16 cells have a doubling time of about 50-60 hr. The cells produced 63 ± 3 ng (mean ± SD) immunoreactive insulin and 9.4 ± 0.3 ng immunoreactive glucagon per day per 106 cells, while somatostatin (SRIF) and pancreatic polypeptide (PP) were undetectable. The reactivity with autoantibodies in IDDM sera was assessed by indirect immunofluorescence or 125I-protein A binding assay on intact cells to detect islet cell... (More)

An insulin-producing cell line, Clone-16, of hamster origin, was characterized for islet hormone production and for reactivity with islet cell surface (ICSA) and islet cell cytoplasmic (ICA) antibodies in sera from children with newly diagnosed insulin-dependent (Type 1) diabetes mellitus (IDDM). The Clone-16 cells have a doubling time of about 50-60 hr. The cells produced 63 ± 3 ng (mean ± SD) immunoreactive insulin and 9.4 ± 0.3 ng immunoreactive glucagon per day per 106 cells, while somatostatin (SRIF) and pancreatic polypeptide (PP) were undetectable. The reactivity with autoantibodies in IDDM sera was assessed by indirect immunofluorescence or 125I-protein A binding assay on intact cells to detect islet cell surface antibodies (ICSA) or on frozen sections of cell platelets to detect islet cell cytoplasmic antibodies (ICCA) by indirect immunofluorescence. Although the proportion of the ICSA-positive Clone-16 cells compared favorably with rat islet cells (r = 0.81; p < 0.01), we found 5/10 IDDM sera to be positive on rat islet cells but 8/10 on the Clone-16 cells. There was also a good correlation in the 125I-protein A binding assay between mouse islet cells and Clone-16 cells (r = 0.91; p < 0.01). Frozen sections of Clone-16 cells showed a cytoplasmic immunofluorescence in 8/10 of the IDDM sera and this reaction paralleled the results obtained in the standard indirect immunofluorescence assay with a frozen section of human blood group O pancreas. We conclude that the insulin- and glucagon-producing Clone-16 cells are a useful cell line for detecting islet cell autoantibodies.

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author
; ; and
publishing date
type
Contribution to journal
publication status
published
subject
in
Diabetes Research
volume
4
issue
3
pages
5 pages
publisher
Teviot-Kimpton
external identifiers
  • pmid:3301157
  • scopus:0023244802
ISSN
0265-5985
language
English
LU publication?
no
id
913a95bd-0441-41b9-a7fe-d12c48c729b8
date added to LUP
2019-09-16 12:29:53
date last changed
2024-03-13 08:14:42
@article{913a95bd-0441-41b9-a7fe-d12c48c729b8,
  abstract     = {{<p>An insulin-producing cell line, Clone-16, of hamster origin, was characterized for islet hormone production and for reactivity with islet cell surface (ICSA) and islet cell cytoplasmic (ICA) antibodies in sera from children with newly diagnosed insulin-dependent (Type 1) diabetes mellitus (IDDM). The Clone-16 cells have a doubling time of about 50-60 hr. The cells produced 63 ± 3 ng (mean ± SD) immunoreactive insulin and 9.4 ± 0.3 ng immunoreactive glucagon per day per 10<sup>6</sup> cells, while somatostatin (SRIF) and pancreatic polypeptide (PP) were undetectable. The reactivity with autoantibodies in IDDM sera was assessed by indirect immunofluorescence or <sup>125</sup>I-protein A binding assay on intact cells to detect islet cell surface antibodies (ICSA) or on frozen sections of cell platelets to detect islet cell cytoplasmic antibodies (ICCA) by indirect immunofluorescence. Although the proportion of the ICSA-positive Clone-16 cells compared favorably with rat islet cells (r = 0.81; p &lt; 0.01), we found 5/10 IDDM sera to be positive on rat islet cells but 8/10 on the Clone-16 cells. There was also a good correlation in the <sup>125</sup>I-protein A binding assay between mouse islet cells and Clone-16 cells (r = 0.91; p &lt; 0.01). Frozen sections of Clone-16 cells showed a cytoplasmic immunofluorescence in 8/10 of the IDDM sera and this reaction paralleled the results obtained in the standard indirect immunofluorescence assay with a frozen section of human blood group O pancreas. We conclude that the insulin- and glucagon-producing Clone-16 cells are a useful cell line for detecting islet cell autoantibodies.</p>}},
  author       = {{Matsuba, I. and Marner, B. and Nerup, J. and Lernmark, A.}},
  issn         = {{0265-5985}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{3}},
  pages        = {{103--107}},
  publisher    = {{Teviot-Kimpton}},
  series       = {{Diabetes Research}},
  title        = {{Detection of islet cell autoantibodies in newly diagnosed diabetic patients using insulin-producing Syrian hamster cells.}},
  volume       = {{4}},
  year         = {{1987}},
}