Nano-Hydroxyapatite Bone Substitute Functionalized with Bone Active Molecules for Enhanced Cranial Bone Regeneration
(2017) In ACS Applied Materials and Interfaces 9(8). p.6816-6828- Abstract
The aim of this study was to synthesize and characterize a nano-hydroxyapatite (nHAP) and calcium sulfate bone substitute (NC) for cranioplasty. The NC was functionalized with low concentrations of bone morphogenetic protein-2 (BMP-2) and zoledronic acid (ZA) and characterized both in vitro and in vivo. In vitro studies included MTT, ALP assays, and fluorescent staining of Saos-2 (human osteoblasts) and MC3T3-E1 (murine preosteoblasts) cells cultured on NC. An in vivo study divided 20 male Wistar rats into four groups: control (defect only), NC, NC + ZA, and NC + ZA + rhBMP-2. The materials were implanted in an 8.5 mm critical size defect in the calvarium for 12 weeks. Micro-CT quantitative analysis was carried out in vivo at 8 weeks... (More)
The aim of this study was to synthesize and characterize a nano-hydroxyapatite (nHAP) and calcium sulfate bone substitute (NC) for cranioplasty. The NC was functionalized with low concentrations of bone morphogenetic protein-2 (BMP-2) and zoledronic acid (ZA) and characterized both in vitro and in vivo. In vitro studies included MTT, ALP assays, and fluorescent staining of Saos-2 (human osteoblasts) and MC3T3-E1 (murine preosteoblasts) cells cultured on NC. An in vivo study divided 20 male Wistar rats into four groups: control (defect only), NC, NC + ZA, and NC + ZA + rhBMP-2. The materials were implanted in an 8.5 mm critical size defect in the calvarium for 12 weeks. Micro-CT quantitative analysis was carried out in vivo at 8 weeks and ex vivo after 12 weeks. Mineralization was highest in the NC + ZA + rhBMP-2 group (13.0 ± 2.8 mm3) compared to the NC + ZA group (9.0 ± 3.2 mm3), NC group (6.4 ± 1.9 mm3), and control group (3.4 ± 1.0 mm3) after 12 weeks. Histological and spectroscopic analysis of the defect site provided a qualitative confirmation of neo-bone, which was in agreement with the micro-CT results. In conclusion, NC can be used as a carrier for bioactive molecules, and functionalization with rhBMP-2 and ZA in low doses enhances bone regeneration.
(Less)
- author
- Teotia, Arun Kumar
; Raina, Deepak Bushan
LU
; Singh, Chandan
; Sinha, Neeraj
; Isaksson, Hanna
LU
; Tägil, Magnus LU ; Lidgren, Lars LU and Kumar, Ashok LU
- organization
- publishing date
- 2017-03-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- bisphosphonates, bone morphogenetic proteins, cranial model, nano-hydroxyapatite, osteoinductive, solid state NMR
- in
- ACS Applied Materials and Interfaces
- volume
- 9
- issue
- 8
- pages
- 13 pages
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- scopus:85014209918
- pmid:28171719
- ISSN
- 1944-8244
- DOI
- 10.1021/acsami.6b14782
- language
- English
- LU publication?
- yes
- id
- 914e9cb4-8bf1-48ff-b742-35e1195fba62
- date added to LUP
- 2017-03-15 07:40:22
- date last changed
- 2025-02-04 14:46:40
@article{914e9cb4-8bf1-48ff-b742-35e1195fba62, abstract = {{<p>The aim of this study was to synthesize and characterize a nano-hydroxyapatite (nHAP) and calcium sulfate bone substitute (NC) for cranioplasty. The NC was functionalized with low concentrations of bone morphogenetic protein-2 (BMP-2) and zoledronic acid (ZA) and characterized both in vitro and in vivo. In vitro studies included MTT, ALP assays, and fluorescent staining of Saos-2 (human osteoblasts) and MC3T3-E1 (murine preosteoblasts) cells cultured on NC. An in vivo study divided 20 male Wistar rats into four groups: control (defect only), NC, NC + ZA, and NC + ZA + rhBMP-2. The materials were implanted in an 8.5 mm critical size defect in the calvarium for 12 weeks. Micro-CT quantitative analysis was carried out in vivo at 8 weeks and ex vivo after 12 weeks. Mineralization was highest in the NC + ZA + rhBMP-2 group (13.0 ± 2.8 mm<sup>3</sup>) compared to the NC + ZA group (9.0 ± 3.2 mm<sup>3</sup>), NC group (6.4 ± 1.9 mm<sup>3</sup>), and control group (3.4 ± 1.0 mm<sup>3</sup>) after 12 weeks. Histological and spectroscopic analysis of the defect site provided a qualitative confirmation of neo-bone, which was in agreement with the micro-CT results. In conclusion, NC can be used as a carrier for bioactive molecules, and functionalization with rhBMP-2 and ZA in low doses enhances bone regeneration.</p>}}, author = {{Teotia, Arun Kumar and Raina, Deepak Bushan and Singh, Chandan and Sinha, Neeraj and Isaksson, Hanna and Tägil, Magnus and Lidgren, Lars and Kumar, Ashok}}, issn = {{1944-8244}}, keywords = {{bisphosphonates; bone morphogenetic proteins; cranial model; nano-hydroxyapatite; osteoinductive; solid state NMR}}, language = {{eng}}, month = {{03}}, number = {{8}}, pages = {{6816--6828}}, publisher = {{The American Chemical Society (ACS)}}, series = {{ACS Applied Materials and Interfaces}}, title = {{Nano-Hydroxyapatite Bone Substitute Functionalized with Bone Active Molecules for Enhanced Cranial Bone Regeneration}}, url = {{http://dx.doi.org/10.1021/acsami.6b14782}}, doi = {{10.1021/acsami.6b14782}}, volume = {{9}}, year = {{2017}}, }