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Red blood cells promote survival and cell cycle progression of human peripheral blood T cells independently of CD58/LFA-3 and heme compounds

Fonseca, Ana Mafalda; Pereira, Carlos Filipe LU ; Porto, Graça and Arosa, Fernando A. (2003) In Cellular Immunology 224(1). p.17-28
Abstract

Red blood cells (RBC) are known to modulate T cell proliferation and function possibly through downregulation of oxidative stress. By examining parameters of activation, division, and cell death in vitro, we show evidence that the increase in survival afforded by RBC is due to the maintenance of the proliferative capacity of the activated T cells. We also show that the CD3+CD8+ T cell subset was preferentially expanded and rescued from apoptosis both in bulk peripheral blood lymphocyte cultures and with highly purified CD8+ T cells. The ability of RBC to induce survival of dividing T cells was not affected by blocking the CD58/CD2 interaction. Moreover, addition of hemoglobin, heme or protoporphyrin IX to cultures of activated T cells... (More)

Red blood cells (RBC) are known to modulate T cell proliferation and function possibly through downregulation of oxidative stress. By examining parameters of activation, division, and cell death in vitro, we show evidence that the increase in survival afforded by RBC is due to the maintenance of the proliferative capacity of the activated T cells. We also show that the CD3+CD8+ T cell subset was preferentially expanded and rescued from apoptosis both in bulk peripheral blood lymphocyte cultures and with highly purified CD8+ T cells. The ability of RBC to induce survival of dividing T cells was not affected by blocking the CD58/CD2 interaction. Moreover, addition of hemoglobin, heme or protoporphyrin IX to cultures of activated T cells did not reproduce the effect of intact RBC. Considering that RBC circulate throughout the body, they could play a biological role in the modulation of T cell differentiation and survival in places of active cell division. Neither CD58 nor the heme compounds studied seem to play a direct relevant role in the modulation of T cell survival.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Apoptosis, CD58/LFA-3, CD8+, Cell cycle, Heme, Red blood cell, Survival, T cell
in
Cellular Immunology
volume
224
issue
1
pages
12 pages
publisher
Elsevier
external identifiers
  • scopus:0142169441
ISSN
0008-8749
DOI
10.1016/S0008-8749(03)00170-9
language
English
LU publication?
no
id
91985edc-246d-4248-859d-ea067c46a6f2
date added to LUP
2017-10-02 17:35:57
date last changed
2018-05-29 09:26:51
@article{91985edc-246d-4248-859d-ea067c46a6f2,
  abstract     = {<p>Red blood cells (RBC) are known to modulate T cell proliferation and function possibly through downregulation of oxidative stress. By examining parameters of activation, division, and cell death in vitro, we show evidence that the increase in survival afforded by RBC is due to the maintenance of the proliferative capacity of the activated T cells. We also show that the CD3+CD8+ T cell subset was preferentially expanded and rescued from apoptosis both in bulk peripheral blood lymphocyte cultures and with highly purified CD8+ T cells. The ability of RBC to induce survival of dividing T cells was not affected by blocking the CD58/CD2 interaction. Moreover, addition of hemoglobin, heme or protoporphyrin IX to cultures of activated T cells did not reproduce the effect of intact RBC. Considering that RBC circulate throughout the body, they could play a biological role in the modulation of T cell differentiation and survival in places of active cell division. Neither CD58 nor the heme compounds studied seem to play a direct relevant role in the modulation of T cell survival.</p>},
  author       = {Fonseca, Ana Mafalda and Pereira, Carlos Filipe and Porto, Graça and Arosa, Fernando A.},
  issn         = {0008-8749},
  keyword      = {Apoptosis,CD58/LFA-3,CD8+,Cell cycle,Heme,Red blood cell,Survival,T cell},
  language     = {eng},
  number       = {1},
  pages        = {17--28},
  publisher    = {Elsevier},
  series       = {Cellular Immunology},
  title        = {Red blood cells promote survival and cell cycle progression of human peripheral blood T cells independently of CD58/LFA-3 and heme compounds},
  url          = {http://dx.doi.org/10.1016/S0008-8749(03)00170-9},
  volume       = {224},
  year         = {2003},
}