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Alterations of Akt1 (PKBalpha) and p70(S6K) in transient focal ischemia

Janelidze, S LU ; Hu, BIng-Ren; Siesjö, P LU and Siesjö, B K LU (2001) In Neurobiology of Disease 8(1). p.147-154
Abstract

The serine-threonine kinase Akt1 promotes cell survival through inhibition of apoptosis. One of the potential downstream targets of Akt1 is p70 S6 kinase, p70(S6K), an enzyme implicated in the regulation of protein synthesis. In this study, we investigated the changes in total and phosphorylated levels of Akt1 and p70(S6K) during transient focal ischemia. Male Wistar rats were subjected to 2 h of middle cerebral artery occlusion followed by 1, 4, and 24 h of reperfusion. The expression of total and phosphorylated forms of Akt1 and p70(S6K) were examined by Western blot analysis. Phosphorylation of Akt1 on Ser473 transiently increased at 1 and 4 h of reperfusion, whereas phosphorylation of Akt1 on Thr308 was reduced during reperfusion.... (More)

The serine-threonine kinase Akt1 promotes cell survival through inhibition of apoptosis. One of the potential downstream targets of Akt1 is p70 S6 kinase, p70(S6K), an enzyme implicated in the regulation of protein synthesis. In this study, we investigated the changes in total and phosphorylated levels of Akt1 and p70(S6K) during transient focal ischemia. Male Wistar rats were subjected to 2 h of middle cerebral artery occlusion followed by 1, 4, and 24 h of reperfusion. The expression of total and phosphorylated forms of Akt1 and p70(S6K) were examined by Western blot analysis. Phosphorylation of Akt1 on Ser473 transiently increased at 1 and 4 h of reperfusion, whereas phosphorylation of Akt1 on Thr308 was reduced during reperfusion. The levels of total Akt1 remained unchanged at 1 and 4 h of reperfusion, but decreased significantly at 24 h of reperfusion. Phosphorylation of p70(S6K) on Thr389 decreased at 1, 4, and 24 h of reperfusion, while the levels of total p70(S6K) protein remained unchanged at 1 and 4 h of reperfusion but decreased at 24 h of reperfusion. The results show that cell survival pathways, such as Akt1 and p70(S6K) signaling, are suppressed after transient focal ischemia, which may contribute to the development of neuronal cell death after an ischemic insult.

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published
subject
keywords
Animals, Arabidopsis Proteins, Blotting, Western, Cytosol/metabolism, Ischemic Attack, Transient/metabolism, Male, Middle Cerebral Artery/physiology, Phosphorylation, Plant Proteins/metabolism, Potassium Channels/metabolism, Rats, Rats, Wistar, Ribosomal Protein S6 Kinases/metabolism
in
Neurobiology of Disease
volume
8
issue
1
pages
147 - 154
publisher
Elsevier
external identifiers
  • scopus:0034745642
ISSN
0969-9961
DOI
10.1006/nbdi.2000.0325
language
English
LU publication?
no
id
91b5cbfd-bdc9-4306-8f56-c356f1f86163
date added to LUP
2019-06-27 10:15:44
date last changed
2019-10-23 06:19:46
@article{91b5cbfd-bdc9-4306-8f56-c356f1f86163,
  abstract     = {<p>The serine-threonine kinase Akt1 promotes cell survival through inhibition of apoptosis. One of the potential downstream targets of Akt1 is p70 S6 kinase, p70(S6K), an enzyme implicated in the regulation of protein synthesis. In this study, we investigated the changes in total and phosphorylated levels of Akt1 and p70(S6K) during transient focal ischemia. Male Wistar rats were subjected to 2 h of middle cerebral artery occlusion followed by 1, 4, and 24 h of reperfusion. The expression of total and phosphorylated forms of Akt1 and p70(S6K) were examined by Western blot analysis. Phosphorylation of Akt1 on Ser473 transiently increased at 1 and 4 h of reperfusion, whereas phosphorylation of Akt1 on Thr308 was reduced during reperfusion. The levels of total Akt1 remained unchanged at 1 and 4 h of reperfusion, but decreased significantly at 24 h of reperfusion. Phosphorylation of p70(S6K) on Thr389 decreased at 1, 4, and 24 h of reperfusion, while the levels of total p70(S6K) protein remained unchanged at 1 and 4 h of reperfusion but decreased at 24 h of reperfusion. The results show that cell survival pathways, such as Akt1 and p70(S6K) signaling, are suppressed after transient focal ischemia, which may contribute to the development of neuronal cell death after an ischemic insult.</p>},
  author       = {Janelidze, S and Hu, BIng-Ren and Siesjö, P and Siesjö, B K},
  issn         = {0969-9961},
  keyword      = {Animals,Arabidopsis Proteins,Blotting, Western,Cytosol/metabolism,Ischemic Attack, Transient/metabolism,Male,Middle Cerebral Artery/physiology,Phosphorylation,Plant Proteins/metabolism,Potassium Channels/metabolism,Rats,Rats, Wistar,Ribosomal Protein S6 Kinases/metabolism},
  language     = {eng},
  number       = {1},
  pages        = {147--154},
  publisher    = {Elsevier},
  series       = {Neurobiology of Disease},
  title        = {Alterations of Akt1 (PKBalpha) and p70(S6K) in transient focal ischemia},
  url          = {http://dx.doi.org/10.1006/nbdi.2000.0325},
  volume       = {8},
  year         = {2001},
}