High resolution UPLC-MS/MS profiling of polyphenolics in the methanol extract of Syzygium samarangense leaves and its hepatoprotective activity in rats with CCl4-induced hepatic damage
Sobeh, Mansour ; Youssef, Fadia S. ; Esmat, Ahmed ; Petruk, Ganna LU
; El-Khatib, Ahmed H.
; Monti, Daria Maria
; Ashour, Mohamed L.
and Wink, Michael
(2018)
In Food and Chemical Toxicology
113.
p.145-153
- Abstract
Oxidative stress plays a crucial role in the development of several liver diseases. Many natural polyphenols can attenuate oxidative stress and liver injury. In this study, a phytochemical profiling of a methanol extract from leaves of Syzygium samarangense revealed 92 compounds belonging to flavonoids, phenolic acids, condensed tannins, and ellagitannins. The S. samarangense extract exhibited a noticeable antioxidant activity with an EC50 of 5.80 μg/mL measured by DPPH scavenging capacity assay, 2632 Trolox equivalents, 10 mM Fe2+ equivalents/mg of samples by TEAC and FRAP assays, respectively. The total phenolic content was 419 mg gallic acid equivalent GAE/g extract. In a cell-based model (HaCaT cells), the... (More)
Oxidative stress plays a crucial role in the development of several liver diseases. Many natural polyphenols can attenuate oxidative stress and liver injury. In this study, a phytochemical profiling of a methanol extract from leaves of Syzygium samarangense revealed 92 compounds belonging to flavonoids, phenolic acids, condensed tannins, and ellagitannins. The S. samarangense extract exhibited a noticeable antioxidant activity with an EC50 of 5.80 μg/mL measured by DPPH scavenging capacity assay, 2632 Trolox equivalents, 10 mM Fe2+ equivalents/mg of samples by TEAC and FRAP assays, respectively. The total phenolic content was 419 mg gallic acid equivalent GAE/g extract. In a cell-based model (HaCaT cells), the extract completely inhibited ROS production induced by UVA, and prevented GSH-depletion and p38 phosphorylation. In addition, the extract exhibited a substantial antioxidant and hepatoprotective activities in CCl4-treated rats, with an increase in GSH (reduced glutathione) and SOD (superoxide dismutase) activities by 84.75 and 26.27%, respectively, and a decrease of 19.08, 63.05, 52.21, 37.00, 13.26, and 15.15% in MDA, ALT, AST, TB (total bilirubin), TC (total cholesterol), and TG (total glycerides), respectively. These results were confirmed by histopathological analyses. We believe that Syzygium samarangense is a good candidate for further evaluation as an antioxidant and liver protecting drug.
(Less)
- author
- Sobeh, Mansour
; Youssef, Fadia S.
; Esmat, Ahmed
; Petruk, Ganna
LU
; El-Khatib, Ahmed H.
; Monti, Daria Maria
; Ashour, Mohamed L.
and Wink, Michael
- publishing date
- 2018-03
- type
- Contribution to journal
- publication status
- published
- keywords
- Antioxidant, Carbon tetrachloride toxicity, HaCaT cells, Hepatoprotective activity, HR-UPLC-MS/MS, Syzygium samarangense
- in
- Food and Chemical Toxicology
- volume
- 113
- pages
- 145 - 153
- publisher
- Elsevier
- external identifiers
-
- pmid:29374594
- scopus:85041563585
- ISSN
- 0278-6915
- DOI
- 10.1016/j.fct.2018.01.031
- language
- English
- LU publication?
- no
- additional info
- Publisher Copyright: © 2018 Elsevier Ltd
- id
- 922b713f-572f-4284-bf4c-3d5846742cfa
- date added to LUP
- 2025-01-21 15:43:59
- date last changed
- 2025-07-09 16:34:34
@article{922b713f-572f-4284-bf4c-3d5846742cfa,
abstract = {{<p>Oxidative stress plays a crucial role in the development of several liver diseases. Many natural polyphenols can attenuate oxidative stress and liver injury. In this study, a phytochemical profiling of a methanol extract from leaves of Syzygium samarangense revealed 92 compounds belonging to flavonoids, phenolic acids, condensed tannins, and ellagitannins. The S. samarangense extract exhibited a noticeable antioxidant activity with an EC<sub>50</sub> of 5.80 μg/mL measured by DPPH scavenging capacity assay, 2632 Trolox equivalents, 10 mM Fe<sup>2+</sup> equivalents/mg of samples by TEAC and FRAP assays, respectively. The total phenolic content was 419 mg gallic acid equivalent GAE/g extract. In a cell-based model (HaCaT cells), the extract completely inhibited ROS production induced by UVA, and prevented GSH-depletion and p38 phosphorylation. In addition, the extract exhibited a substantial antioxidant and hepatoprotective activities in CCl<sub>4</sub>-treated rats, with an increase in GSH (reduced glutathione) and SOD (superoxide dismutase) activities by 84.75 and 26.27%, respectively, and a decrease of 19.08, 63.05, 52.21, 37.00, 13.26, and 15.15% in MDA, ALT, AST, TB (total bilirubin), TC (total cholesterol), and TG (total glycerides), respectively. These results were confirmed by histopathological analyses. We believe that Syzygium samarangense is a good candidate for further evaluation as an antioxidant and liver protecting drug.</p>}},
author = {{Sobeh, Mansour and Youssef, Fadia S. and Esmat, Ahmed and Petruk, Ganna and El-Khatib, Ahmed H. and Monti, Daria Maria and Ashour, Mohamed L. and Wink, Michael}},
issn = {{0278-6915}},
keywords = {{Antioxidant; Carbon tetrachloride toxicity; HaCaT cells; Hepatoprotective activity; HR-UPLC-MS/MS; Syzygium samarangense}},
language = {{eng}},
pages = {{145--153}},
publisher = {{Elsevier}},
series = {{Food and Chemical Toxicology}},
title = {{High resolution UPLC-MS/MS profiling of polyphenolics in the methanol extract of Syzygium samarangense leaves and its hepatoprotective activity in rats with CCl<sub>4</sub>-induced hepatic damage}},
url = {{http://dx.doi.org/10.1016/j.fct.2018.01.031}},
doi = {{10.1016/j.fct.2018.01.031}},
volume = {{113}},
year = {{2018}},
}