DamID transcriptional profiling identifies the Snail/Scratch transcription factor Kahuli as an Alk target in the Drosophila visceral mesoderm
(2021) In Development: For advances in developmental biology and stem cells 148(23).- Abstract
Development of the Drosophila visceral muscle depends on Anaplastic Lymphoma Kinase (Alk) receptor tyrosine kinase (RTK) signaling, which specifies founder cells (FCs) in the circular visceral mesoderm (VM). Although Alk activation by its ligand Jelly Belly (Jeb) is well characterized, few target molecules have been identified. Here, we used targeted DamID (TaDa) to identify Alk targets in embryos overexpressing Jeb versus embryos with abrogated Alk activity, revealing differentially expressed genes, including the Snail/Scratch family transcription factor Kahuli (Kah). We confirmed Kah mRNA and protein expression in the VM, and identified midgut constriction defects in Kah mutants similar to those of pointed (pnt). ChIP and RNA-Seq data... (More)
Development of the Drosophila visceral muscle depends on Anaplastic Lymphoma Kinase (Alk) receptor tyrosine kinase (RTK) signaling, which specifies founder cells (FCs) in the circular visceral mesoderm (VM). Although Alk activation by its ligand Jelly Belly (Jeb) is well characterized, few target molecules have been identified. Here, we used targeted DamID (TaDa) to identify Alk targets in embryos overexpressing Jeb versus embryos with abrogated Alk activity, revealing differentially expressed genes, including the Snail/Scratch family transcription factor Kahuli (Kah). We confirmed Kah mRNA and protein expression in the VM, and identified midgut constriction defects in Kah mutants similar to those of pointed (pnt). ChIP and RNA-Seq data analysis defined a Kah target-binding site similar to that of Snail, and identified a set of common target genes putatively regulated by Kah and Pnt during midgut constriction. Taken together, we report a rich dataset of Alk-responsive loci in the embryonic VM and functionally characterize the role of Kah in the regulation of embryonic midgut morphogenesis.
(Less)
- author
- Mendoza-Garcia, Patricia
; Basu, Swaraj
; Sukumar, Sanjay Kumar
; Arefin, Badrul
; Wolfstetter, Georg
; Anthonydhason, Vimala
; Molander, Linnea
; Uçkun, Ezgi
; Lindehell, Henrik
; Lebrero-Fernandez, Cristina
; Larsson, Jan
; Larsson, Erik
; Bemark, Mats
LU
and Palmer, Ruth H
(Less) - publishing date
- 2021-12-01
- type
- Contribution to journal
- publication status
- published
- keywords
- Animals, Anaplastic Lymphoma Kinase/genetics, Cell Differentiation/genetics, DNA-Binding Proteins/genetics, Drosophila melanogaster/genetics, Drosophila Proteins/genetics, Embryonic Development/genetics, Gene Expression Profiling, Gene Expression Regulation, Developmental/genetics, Mesoderm/growth & development, Muscle Development/genetics, Muscles/metabolism, Nerve Tissue Proteins/genetics, Proto-Oncogene Proteins/genetics, RNA, Messenger/genetics, RNA-Seq, Signal Transduction/genetics, Single-Cell Analysis, Transcription Factors/genetics
- in
- Development: For advances in developmental biology and stem cells
- volume
- 148
- issue
- 23
- article number
- 199465
- publisher
- The Company of Biologists Ltd
- external identifiers
-
- scopus:85122474160
- pmid:34905617
- ISSN
- 1477-9129
- DOI
- 10.1242/dev.199465
- language
- English
- LU publication?
- no
- additional info
- © 2021. Published by The Company of Biologists Ltd.
- id
- 92507348-07d6-4752-9a07-9f54394c566a
- date added to LUP
- 2023-11-16 12:53:18
- date last changed
- 2024-04-29 18:23:16
@article{92507348-07d6-4752-9a07-9f54394c566a,
abstract = {{<p>Development of the Drosophila visceral muscle depends on Anaplastic Lymphoma Kinase (Alk) receptor tyrosine kinase (RTK) signaling, which specifies founder cells (FCs) in the circular visceral mesoderm (VM). Although Alk activation by its ligand Jelly Belly (Jeb) is well characterized, few target molecules have been identified. Here, we used targeted DamID (TaDa) to identify Alk targets in embryos overexpressing Jeb versus embryos with abrogated Alk activity, revealing differentially expressed genes, including the Snail/Scratch family transcription factor Kahuli (Kah). We confirmed Kah mRNA and protein expression in the VM, and identified midgut constriction defects in Kah mutants similar to those of pointed (pnt). ChIP and RNA-Seq data analysis defined a Kah target-binding site similar to that of Snail, and identified a set of common target genes putatively regulated by Kah and Pnt during midgut constriction. Taken together, we report a rich dataset of Alk-responsive loci in the embryonic VM and functionally characterize the role of Kah in the regulation of embryonic midgut morphogenesis.</p>}},
author = {{Mendoza-Garcia, Patricia and Basu, Swaraj and Sukumar, Sanjay Kumar and Arefin, Badrul and Wolfstetter, Georg and Anthonydhason, Vimala and Molander, Linnea and Uçkun, Ezgi and Lindehell, Henrik and Lebrero-Fernandez, Cristina and Larsson, Jan and Larsson, Erik and Bemark, Mats and Palmer, Ruth H}},
issn = {{1477-9129}},
keywords = {{Animals; Anaplastic Lymphoma Kinase/genetics; Cell Differentiation/genetics; DNA-Binding Proteins/genetics; Drosophila melanogaster/genetics; Drosophila Proteins/genetics; Embryonic Development/genetics; Gene Expression Profiling; Gene Expression Regulation, Developmental/genetics; Mesoderm/growth & development; Muscle Development/genetics; Muscles/metabolism; Nerve Tissue Proteins/genetics; Proto-Oncogene Proteins/genetics; RNA, Messenger/genetics; RNA-Seq; Signal Transduction/genetics; Single-Cell Analysis; Transcription Factors/genetics}},
language = {{eng}},
month = {{12}},
number = {{23}},
publisher = {{The Company of Biologists Ltd}},
series = {{Development: For advances in developmental biology and stem cells}},
title = {{DamID transcriptional profiling identifies the Snail/Scratch transcription factor Kahuli as an Alk target in the Drosophila visceral mesoderm}},
url = {{http://dx.doi.org/10.1242/dev.199465}},
doi = {{10.1242/dev.199465}},
volume = {{148}},
year = {{2021}},
}