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Synthesis and characterization of molecularly imprinted microspheres

Ye, Lei LU ; Weiss, Robert and Mosbach, Klaus LU (2000) In Macromolecules 33(22). p.8239-8245
Abstract

A novel method for the preparation of molecularly imprinted microspheres has been developed. Theophylline, caffeine, and 17β-estradiol are used as template molecules to demonstrate the generally applicable methodology. In the presence of a template molecule, cross-linking polymerization starting from a dilute solution of methacrylic acid and trimethylolpropane trimethacrylate yields polymer microspheres bearing imprinted binding sites. Radioligand binding analysis confirms that very high binding specificity is retained when the molar ratio of functional monomer: cross-linker is controlled between 1:1 and 2.3:1. Nonspecific binding of molecularly imprinted microspheres decreases when an increasing amount of template molecule is used for... (More)

A novel method for the preparation of molecularly imprinted microspheres has been developed. Theophylline, caffeine, and 17β-estradiol are used as template molecules to demonstrate the generally applicable methodology. In the presence of a template molecule, cross-linking polymerization starting from a dilute solution of methacrylic acid and trimethylolpropane trimethacrylate yields polymer microspheres bearing imprinted binding sites. Radioligand binding analysis confirms that very high binding specificity is retained when the molar ratio of functional monomer: cross-linker is controlled between 1:1 and 2.3:1. Nonspecific binding of molecularly imprinted microspheres decreases when an increasing amount of template molecule is used for polymer preparation. Compared with previously reported suspension polymerization and seed polymerization, the present method is highly efficient, is generally applicable to various template molecules, and gives uniform microspheres in good yield.

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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Macromolecules
volume
33
issue
22
pages
7 pages
publisher
The American Chemical Society (ACS)
external identifiers
  • scopus:0034291592
ISSN
0024-9297
DOI
10.1021/ma000825t
language
English
LU publication?
yes
id
92ef7ee1-bff4-4696-871b-ec26fef55b7a
date added to LUP
2019-06-18 09:08:43
date last changed
2020-12-08 02:06:24
@article{92ef7ee1-bff4-4696-871b-ec26fef55b7a,
  abstract     = {<p>A novel method for the preparation of molecularly imprinted microspheres has been developed. Theophylline, caffeine, and 17β-estradiol are used as template molecules to demonstrate the generally applicable methodology. In the presence of a template molecule, cross-linking polymerization starting from a dilute solution of methacrylic acid and trimethylolpropane trimethacrylate yields polymer microspheres bearing imprinted binding sites. Radioligand binding analysis confirms that very high binding specificity is retained when the molar ratio of functional monomer: cross-linker is controlled between 1:1 and 2.3:1. Nonspecific binding of molecularly imprinted microspheres decreases when an increasing amount of template molecule is used for polymer preparation. Compared with previously reported suspension polymerization and seed polymerization, the present method is highly efficient, is generally applicable to various template molecules, and gives uniform microspheres in good yield.</p>},
  author       = {Ye, Lei and Weiss, Robert and Mosbach, Klaus},
  issn         = {0024-9297},
  language     = {eng},
  month        = {10},
  number       = {22},
  pages        = {8239--8245},
  publisher    = {The American Chemical Society (ACS)},
  series       = {Macromolecules},
  title        = {Synthesis and characterization of molecularly imprinted microspheres},
  url          = {http://dx.doi.org/10.1021/ma000825t},
  doi          = {10.1021/ma000825t},
  volume       = {33},
  year         = {2000},
}