From Genotypes of Immunoglobulin Constant Heavy G Chains (Fcγ) (GM) Genes (IGHG) to Phenotypes in Childhood Asthma.
(2012) In International Archives of Allergy and Immunology 159(1). p.94-102- Abstract
- Background:
IgE-mediated allergy is associated with immunoglobulin heavy constant G chain (Fcγ) (GM) genes (IGHG) on chromosome 14q32.3. Investigation of the alternative GM allotypes of γ3, γ1 and γ2 chains has disclosed new structural and functional IgG subclasses and B-cell variants, with possible effects on childhood asthma.
Objective:
To investigate different IGHG (GM) gene complexes in a childhood asthma population for allergy parameters.
Methods:
IGHG alleles and correlated allotypic (allelic) IgG subclass levels were analyzed with a sensitive indirect competitive ELISA in 10-year-old children with bronchial asthma. Individual IGHG diplotype-, genotype- and... (More) - Background:
IgE-mediated allergy is associated with immunoglobulin heavy constant G chain (Fcγ) (GM) genes (IGHG) on chromosome 14q32.3. Investigation of the alternative GM allotypes of γ3, γ1 and γ2 chains has disclosed new structural and functional IgG subclasses and B-cell variants, with possible effects on childhood asthma.
Objective:
To investigate different IGHG (GM) gene complexes in a childhood asthma population for allergy parameters.
Methods:
IGHG alleles and correlated allotypic (allelic) IgG subclass levels were analyzed with a sensitive indirect competitive ELISA in 10-year-old children with bronchial asthma. Individual IGHG diplotype-, genotype- and haplotype-related B cells were compared for allelic IgG subclass levels, IgE sensitization, IgE, IgA and IgM levels, and numbers of peripheral blood eosinophils and lymphocytes.
Results:
The group with homozygous IGHG*bfn/*bfn (B1/B1 cells) demonstrated low IgG1*f levels (p < 0.001) but increased IgG2*n levels (p < 0.001) together with increasd IgE and IGHG2*n gene dose-dependent IgE sensitization (atopic phenotype). The IGHG*bf-n/*bf-n (B2/B2 cells) demonstrated low IgG1*f (p < 0.05) and IgG2*-n (p < 0.001) and the IGHG*ga-n/*ga-n (B4/B4 cells) low IgG1*a (p < 0.001) and IgG2*-n (p < 0.02) together with low IgE sensitization (non-atopic phenotype). B*(bfn) (B1) and B*(bf-n) (B2) demonstrated increased numbers of peripheral blood eosinophils, compared to B*(gan) (B4) cells, which demonstrated increased peripheral blood CD8 lymphocytes instead.
Conclusion:
IGHG diplotypes present different phenotypes in childhood asthma. The IGHG2*n dose relationship to IgE sensitization and increased IgG2*n levels in IgE sensitized are risk markers for IgE-mediated asthma. The opposite IGHG2*-n presents non-IgE-mediated asthma and IgG subclass deficiencies. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2608967
- author
- Oxelius, Vivi-Anne LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Fc gamma, Allelic IgG subclasses, GM allotypes, IgE, Immunodeficiency, Phenotypes, IGHG diagnostic tests, Immunoglobulin constant heavy G chain
- in
- International Archives of Allergy and Immunology
- volume
- 159
- issue
- 1
- pages
- 94 - 102
- publisher
- Karger
- external identifiers
-
- wos:000305801000012
- pmid:22573066
- scopus:84860871538
- pmid:22573066
- ISSN
- 1423-0097
- DOI
- 10.1159/000335592
- language
- English
- LU publication?
- yes
- id
- 934a4b86-e958-4f39-b673-16789fa07588 (old id 2608967)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/22573066?dopt=Abstract
- date added to LUP
- 2016-04-01 11:03:22
- date last changed
- 2025-04-04 15:27:27
@article{934a4b86-e958-4f39-b673-16789fa07588,
abstract = {{Background: <br/><br>
IgE-mediated allergy is associated with immunoglobulin heavy constant G chain (Fcγ) (GM) genes (IGHG) on chromosome 14q32.3. Investigation of the alternative GM allotypes of γ3, γ1 and γ2 chains has disclosed new structural and functional IgG subclasses and B-cell variants, with possible effects on childhood asthma. <br/><br>
<br/><br>
Objective: <br/><br>
To investigate different IGHG (GM) gene complexes in a childhood asthma population for allergy parameters. <br/><br>
<br/><br>
Methods: <br/><br>
IGHG alleles and correlated allotypic (allelic) IgG subclass levels were analyzed with a sensitive indirect competitive ELISA in 10-year-old children with bronchial asthma. Individual IGHG diplotype-, genotype- and haplotype-related B cells were compared for allelic IgG subclass levels, IgE sensitization, IgE, IgA and IgM levels, and numbers of peripheral blood eosinophils and lymphocytes. <br/><br>
<br/><br>
Results: <br/><br>
The group with homozygous IGHG*bfn/*bfn (B1/B1 cells) demonstrated low IgG1*f levels (p < 0.001) but increased IgG2*n levels (p < 0.001) together with increasd IgE and IGHG2*n gene dose-dependent IgE sensitization (atopic phenotype). The IGHG*bf-n/*bf-n (B2/B2 cells) demonstrated low IgG1*f (p < 0.05) and IgG2*-n (p < 0.001) and the IGHG*ga-n/*ga-n (B4/B4 cells) low IgG1*a (p < 0.001) and IgG2*-n (p < 0.02) together with low IgE sensitization (non-atopic phenotype). B*(bfn) (B1) and B*(bf-n) (B2) demonstrated increased numbers of peripheral blood eosinophils, compared to B*(gan) (B4) cells, which demonstrated increased peripheral blood CD8 lymphocytes instead. <br/><br>
<br/><br>
Conclusion: <br/><br>
IGHG diplotypes present different phenotypes in childhood asthma. The IGHG2*n dose relationship to IgE sensitization and increased IgG2*n levels in IgE sensitized are risk markers for IgE-mediated asthma. The opposite IGHG2*-n presents non-IgE-mediated asthma and IgG subclass deficiencies.}},
author = {{Oxelius, Vivi-Anne}},
issn = {{1423-0097}},
keywords = {{Fc gamma; Allelic IgG subclasses; GM allotypes; IgE; Immunodeficiency; Phenotypes; IGHG diagnostic tests; Immunoglobulin constant heavy G chain}},
language = {{eng}},
number = {{1}},
pages = {{94--102}},
publisher = {{Karger}},
series = {{International Archives of Allergy and Immunology}},
title = {{From Genotypes of Immunoglobulin Constant Heavy G Chains (Fcγ) (GM) Genes (IGHG) to Phenotypes in Childhood Asthma.}},
url = {{http://dx.doi.org/10.1159/000335592}},
doi = {{10.1159/000335592}},
volume = {{159}},
year = {{2012}},
}