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Effect of kidney stem cells on inflammatory factors in rats with diabetic nephropathy

Rafiee, Zeinab LU ; Orazizadeh, Mahmoud and Mansouri, Esrafil (2021) 6th world congress of the Tissue Engineering and Regenerative Medicine International Society (TERMIS2021) p.1091-1091
Abstract
Mesenchymal stem cells (MSCs) were proposed as a critical therapeutic candidate in diabetic nephropathy (DN). Renal stem cells as a source for repairing are controversial. The purpose of the present study was to evaluate the effect of kidney rat stem cells on DN. After evaluation of surface stem cell markers by flow cytometry analysis, MSCs that were labeled with CM-Dil were injected to experimental groups via tail vein (2×106cells/rat). To establish the diabetic nephropathy rat model STZ(60mg/kg) was used. The IL1-β, TGF-β, miRNA 192, miRNA29a gene expression were assessed three weeks after induction of early diabetic nephropathy by using quantitative Real-time PCR. The renal stem cells 24 h after labeling with CM-Dil fluorescent dye were... (More)
Mesenchymal stem cells (MSCs) were proposed as a critical therapeutic candidate in diabetic nephropathy (DN). Renal stem cells as a source for repairing are controversial. The purpose of the present study was to evaluate the effect of kidney rat stem cells on DN. After evaluation of surface stem cell markers by flow cytometry analysis, MSCs that were labeled with CM-Dil were injected to experimental groups via tail vein (2×106cells/rat). To establish the diabetic nephropathy rat model STZ(60mg/kg) was used. The IL1-β, TGF-β, miRNA 192, miRNA29a gene expression were assessed three weeks after induction of early diabetic nephropathy by using quantitative Real-time PCR. The renal stem cells 24 h after labeling with CM-Dil fluorescent dye were detected in the renal tissues. miRNA192, TGF-β and IL1-β Genes expression upregulated in diabetic group. The expression of miRNA29a significantly down-regulated 21 days after injection of STZ. H and E staining showed in the presence of adult kidney stem cells histopathological changes were improved. The protective effect of MSCs was closely related to the inactivation of inflammatory factors. Therefore, the MSCs may be a promising novel treatment for early DN. (Less)
Please use this url to cite or link to this publication:
author
; and
publishing date
type
Contribution to conference
publication status
published
keywords
stem cell therapy, Inflammatory cytokines, Diabetic nephropathy
pages
1091 - 1091
conference name
6th world congress of the Tissue Engineering and Regenerative Medicine International Society (TERMIS2021)
conference location
Maastricht, Netherlands
conference dates
2021-11-15 - 2021-11-19
language
English
LU publication?
no
id
93865ba4-d47b-4b65-878b-16eb66394de9
alternative location
https://termis.org/sites/default/files/20211207%20TERMIS%202021%20ABSTRACT%20BOOK%20RG%20v6.pdf
date added to LUP
2021-09-25 11:03:09
date last changed
2021-12-28 08:18:31
@misc{93865ba4-d47b-4b65-878b-16eb66394de9,
  abstract     = {{Mesenchymal stem cells (MSCs) were proposed as a critical therapeutic candidate in diabetic nephropathy (DN). Renal stem cells as a source for repairing are controversial. The purpose of the present study was to evaluate the effect of kidney rat stem cells on DN. After evaluation of surface stem cell markers by flow cytometry analysis, MSCs that were labeled with CM-Dil were injected to experimental groups via tail vein (2×106cells/rat). To establish the diabetic nephropathy rat model STZ(60mg/kg) was used. The IL1-β, TGF-β, miRNA 192, miRNA29a gene expression were assessed three weeks after induction of early diabetic nephropathy by using quantitative Real-time PCR. The renal stem cells 24 h after labeling with CM-Dil fluorescent dye were detected in the renal tissues. miRNA192, TGF-β and IL1-β Genes expression upregulated in diabetic group. The expression of miRNA29a significantly down-regulated 21 days after injection of STZ. H and E staining showed in the presence of adult kidney stem cells histopathological changes were improved. The protective effect of MSCs was closely related to the inactivation of inflammatory factors. Therefore, the MSCs may be a promising novel treatment for early DN.}},
  author       = {{Rafiee, Zeinab and Orazizadeh, Mahmoud and Mansouri, Esrafil}},
  keywords     = {{stem cell therapy; Inflammatory cytokines; Diabetic nephropathy}},
  language     = {{eng}},
  pages        = {{1091--1091}},
  title        = {{Effect of kidney stem cells on inflammatory factors in rats with diabetic nephropathy}},
  url          = {{https://termis.org/sites/default/files/20211207%20TERMIS%202021%20ABSTRACT%20BOOK%20RG%20v6.pdf}},
  year         = {{2021}},
}