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A Systematic Characterization of Aquaporin-9 Expression in Human Normal and Pathological Tissues

Lindskog, Cecilia; Asplund, Anna; Catrina, Anca; Nielsen, Søren and Rützler, Michael (2016) In The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 64(5). p.287-300
Abstract

AQP9 is known to facilitate hepatocyte glycerol uptake. Murine AQP9 protein expression has been verified in liver, skin, epididymis, epidermis and neuronal cells using knockout mice. Further expression sites have been reported in humans. We aimed to verify AQP9 expression in a large set of human normal organs, different cancer types, rheumatoid arthritis synovial biopsies as well as in cell lines and primary cells. Combining standardized immunohistochemistry with high-throughput mRNA sequencing, we found that AQP9 expression in normal tissues was limited, with high membranous expression only in hepatocytes. In cancer tissues, AQP9 expression was mainly found in hepatocellular carcinomas, suggesting no general contribution of AQP9 to... (More)

AQP9 is known to facilitate hepatocyte glycerol uptake. Murine AQP9 protein expression has been verified in liver, skin, epididymis, epidermis and neuronal cells using knockout mice. Further expression sites have been reported in humans. We aimed to verify AQP9 expression in a large set of human normal organs, different cancer types, rheumatoid arthritis synovial biopsies as well as in cell lines and primary cells. Combining standardized immunohistochemistry with high-throughput mRNA sequencing, we found that AQP9 expression in normal tissues was limited, with high membranous expression only in hepatocytes. In cancer tissues, AQP9 expression was mainly found in hepatocellular carcinomas, suggesting no general contribution of AQP9 to carcinogenesis. AQP9 expression in a subset of rheumatoid arthritis synovial tissue samples was affected by Humira, thereby supporting a suggested role of TNFα in AQP9 regulation in this disease. Among cell lines and primary cells, LP-1 myeloma cells expressed high levels of AQP9, whereas low expression was observed in a few other lymphoid cell lines. AQP9 mRNA and protein expression was absent in HepG2 hepatocellular carcinoma cells. Overall, AQP9 expression in human tissues appears to be more selective than in mice.

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author
publishing date
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Contribution to journal
publication status
published
subject
keywords
aquaporin, HepG2, Humira, LP-1, TNF-alpha
in
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
volume
64
issue
5
pages
14 pages
publisher
Histochemical Society
external identifiers
  • scopus:84964917253
  • wos:000375128100001
ISSN
0022-1554
DOI
10.1369/0022155416641028
language
English
LU publication?
no
id
938f040d-9527-42ee-a4cb-5ad147db2ff4
date added to LUP
2016-06-29 14:45:33
date last changed
2017-08-27 06:20:36
@article{938f040d-9527-42ee-a4cb-5ad147db2ff4,
  abstract     = {<p>AQP9 is known to facilitate hepatocyte glycerol uptake. Murine AQP9 protein expression has been verified in liver, skin, epididymis, epidermis and neuronal cells using knockout mice. Further expression sites have been reported in humans. We aimed to verify AQP9 expression in a large set of human normal organs, different cancer types, rheumatoid arthritis synovial biopsies as well as in cell lines and primary cells. Combining standardized immunohistochemistry with high-throughput mRNA sequencing, we found that AQP9 expression in normal tissues was limited, with high membranous expression only in hepatocytes. In cancer tissues, AQP9 expression was mainly found in hepatocellular carcinomas, suggesting no general contribution of AQP9 to carcinogenesis. AQP9 expression in a subset of rheumatoid arthritis synovial tissue samples was affected by Humira, thereby supporting a suggested role of TNFα in AQP9 regulation in this disease. Among cell lines and primary cells, LP-1 myeloma cells expressed high levels of AQP9, whereas low expression was observed in a few other lymphoid cell lines. AQP9 mRNA and protein expression was absent in HepG2 hepatocellular carcinoma cells. Overall, AQP9 expression in human tissues appears to be more selective than in mice.</p>},
  author       = {Lindskog, Cecilia and Asplund, Anna and Catrina, Anca and Nielsen, Søren and Rützler, Michael},
  issn         = {0022-1554},
  keyword      = {aquaporin,HepG2,Humira,LP-1,TNF-alpha},
  language     = {eng},
  number       = {5},
  pages        = {287--300},
  publisher    = {Histochemical Society},
  series       = {The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  title        = {A Systematic Characterization of Aquaporin-9 Expression in Human Normal and Pathological Tissues},
  url          = {http://dx.doi.org/10.1369/0022155416641028},
  volume       = {64},
  year         = {2016},
}