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PCR-mediated deletion of plasmid DNA

Hansson, Mattias LU ; Rzeznicka, Kamila LU ; Rosenbäck, Matilda; Hansson, Mats LU and Sirijovski, Nick LU (2008) In Analytical Biochemistry 375(2). p.373-375
Abstract
The PCR-mediated plasmid DNA deletion method is a simple approach to delete DNA sequences from plasmids using only one round of PCR, with two primers, and without ligation or purification prior to in vivo recombination. By using only PCR, the method is sequence independent and, as shown in this study, is applicable to various sizes of plasmids and deletions using minimal primer design.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Biochemistry
volume
375
issue
2
pages
373 - 375
publisher
Elsevier
external identifiers
  • pmid:18157935
  • wos:000254410800029
  • scopus:40649102417
ISSN
1096-0309
DOI
10.1016/j.ab.2007.12.005
language
English
LU publication?
yes
id
19741943-5a8d-4bd5-91b4-94f37a229269 (old id 939116)
date added to LUP
2008-01-21 10:54:10
date last changed
2017-08-13 03:29:21
@article{19741943-5a8d-4bd5-91b4-94f37a229269,
  abstract     = {The PCR-mediated plasmid DNA deletion method is a simple approach to delete DNA sequences from plasmids using only one round of PCR, with two primers, and without ligation or purification prior to in vivo recombination. By using only PCR, the method is sequence independent and, as shown in this study, is applicable to various sizes of plasmids and deletions using minimal primer design.},
  author       = {Hansson, Mattias and Rzeznicka, Kamila and Rosenbäck, Matilda and Hansson, Mats and Sirijovski, Nick},
  issn         = {1096-0309},
  language     = {eng},
  number       = {2},
  pages        = {373--375},
  publisher    = {Elsevier},
  series       = {Analytical Biochemistry},
  title        = {PCR-mediated deletion of plasmid DNA},
  url          = {http://dx.doi.org/10.1016/j.ab.2007.12.005},
  volume       = {375},
  year         = {2008},
}