Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Ropivacaine and lidocaine at clinically relevant concentrations suppress proliferation and migration of ovarian cancer cells and induce morphological alterations

Hayden, Jane M. ; Tinnert, Andreas LU ; Alm, Kersti LU ; Oras, Jonatan ; Block, Linda ; Gupta, Anil ; Thörn, Sven Egron and Oredsson, Stina LU (2025) In Advances in Medical Sciences 70(2). p.326-334
Abstract

Purpose: Ovarian cancer ranks as a gynecological malignancy with poor prognosis, specifically if detected late. Primary treatment includes cytoreductive surgery and adjuvant chemotherapy with curative intent. Local anesthetics (LA) administered in the perioperative period may potentially impact patient outcome by several mechanisms. The beneficial impact of LA has been attributed, among other factors, to the drug's inhibitory effect on cancer cells. The primary aim of the study was to evaluate the effect of clinically relevant concentrations of ropivacaine and lidocaine on ovarian cancer cell lines. Method: Three ovarian cancer cell lines (SKOV-3, SW-626 and CA-OV-3) were treated with 1, 10, 100, or 1000 ​μM of the two LAs. Cell... (More)

Purpose: Ovarian cancer ranks as a gynecological malignancy with poor prognosis, specifically if detected late. Primary treatment includes cytoreductive surgery and adjuvant chemotherapy with curative intent. Local anesthetics (LA) administered in the perioperative period may potentially impact patient outcome by several mechanisms. The beneficial impact of LA has been attributed, among other factors, to the drug's inhibitory effect on cancer cells. The primary aim of the study was to evaluate the effect of clinically relevant concentrations of ropivacaine and lidocaine on ovarian cancer cell lines. Method: Three ovarian cancer cell lines (SKOV-3, SW-626 and CA-OV-3) were treated with 1, 10, 100, or 1000 ​μM of the two LAs. Cell function and morphology were assessed in the following ways: cell counting, phase-contrast and holographic microscopy, a conventional MTT assay for dose response testing, wound healing assay for migration, and cancer stem cell (CSC) identification by aldehyde dehydrogenase. Results: Both ropivacaine and lidocaine significantly reduced cell number, altered morphology, suppressed migration, and decreased the population of CSCs in a concentration-dependent manner. Conclusion: LAs exert a direct inhibitory effect on ovarian cancer cell lines in vitro, suggesting their potential benefits in perioperative management for patients undergoing surgery. Clinical studies using LA during ovarian cancer surgery are needed.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cancer stem cells, Local anesthetics, Molecular targeted therapy, Ovarian cancer, Ovarian cancer cell lines
in
Advances in Medical Sciences
volume
70
issue
2
pages
9 pages
publisher
Medical University of Bialystok
external identifiers
  • scopus:105015424948
  • pmid:40930388
ISSN
1896-1126
DOI
10.1016/j.advms.2025.08.003
language
English
LU publication?
yes
id
94017beb-f1bb-4748-b1d9-87b70cbf28b6
date added to LUP
2025-10-10 12:28:51
date last changed
2025-10-24 13:56:53
@article{94017beb-f1bb-4748-b1d9-87b70cbf28b6,
  abstract     = {{<p>Purpose: Ovarian cancer ranks as a gynecological malignancy with poor prognosis, specifically if detected late. Primary treatment includes cytoreductive surgery and adjuvant chemotherapy with curative intent. Local anesthetics (LA) administered in the perioperative period may potentially impact patient outcome by several mechanisms. The beneficial impact of LA has been attributed, among other factors, to the drug's inhibitory effect on cancer cells. The primary aim of the study was to evaluate the effect of clinically relevant concentrations of ropivacaine and lidocaine on ovarian cancer cell lines. Method: Three ovarian cancer cell lines (SKOV-3, SW-626 and CA-OV-3) were treated with 1, 10, 100, or 1000 ​μM of the two LAs. Cell function and morphology were assessed in the following ways: cell counting, phase-contrast and holographic microscopy, a conventional MTT assay for dose response testing, wound healing assay for migration, and cancer stem cell (CSC) identification by aldehyde dehydrogenase. Results: Both ropivacaine and lidocaine significantly reduced cell number, altered morphology, suppressed migration, and decreased the population of CSCs in a concentration-dependent manner. Conclusion: LAs exert a direct inhibitory effect on ovarian cancer cell lines in vitro, suggesting their potential benefits in perioperative management for patients undergoing surgery. Clinical studies using LA during ovarian cancer surgery are needed.</p>}},
  author       = {{Hayden, Jane M. and Tinnert, Andreas and Alm, Kersti and Oras, Jonatan and Block, Linda and Gupta, Anil and Thörn, Sven Egron and Oredsson, Stina}},
  issn         = {{1896-1126}},
  keywords     = {{Cancer stem cells; Local anesthetics; Molecular targeted therapy; Ovarian cancer; Ovarian cancer cell lines}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{326--334}},
  publisher    = {{Medical University of Bialystok}},
  series       = {{Advances in Medical Sciences}},
  title        = {{Ropivacaine and lidocaine at clinically relevant concentrations suppress proliferation and migration of ovarian cancer cells and induce morphological alterations}},
  url          = {{http://dx.doi.org/10.1016/j.advms.2025.08.003}},
  doi          = {{10.1016/j.advms.2025.08.003}},
  volume       = {{70}},
  year         = {{2025}},
}