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Mutagenic and functional analysis of the C-terminus of Saccharomyces cerevisiae Pho84 phosphate transporter

Lagerstedt, Jens O. LU ; Zvyagilskaya, Renata; Pratt, James R; Pattison-Granberg, Johanna; Kruckeberg, Arthur L; Berden, Jan A and Persson, Bengt L (2002) In FEBS Letters 526(1-3). p.7-31
Abstract

A widely accepted mechanism for selective degradation of plasma membrane proteins is via ubiquitination and/or phosphorylation events. Such a regulated degradation has previously been suggested to rely on the presence of a specific SINNDAKSS sequence within the protein. Modification of a partly conserved SINNDAKSS-like sequence in the C-terminal tail of the Pho84 phosphate transporter, in combination with C-terminal fusion of green fluorescent protein or a MYC epitope, were used to evaluate the presence of this sequence and its role in the regulated degradation. The functional Pho84 mutants in which this SINNDAKSS-like sequence was altered or truncated were subjected to degradation like that of the wild type, suggesting that degradation... (More)

A widely accepted mechanism for selective degradation of plasma membrane proteins is via ubiquitination and/or phosphorylation events. Such a regulated degradation has previously been suggested to rely on the presence of a specific SINNDAKSS sequence within the protein. Modification of a partly conserved SINNDAKSS-like sequence in the C-terminal tail of the Pho84 phosphate transporter, in combination with C-terminal fusion of green fluorescent protein or a MYC epitope, were used to evaluate the presence of this sequence and its role in the regulated degradation. The functional Pho84 mutants in which this SINNDAKSS-like sequence was altered or truncated were subjected to degradation like that of the wild type, suggesting that degradation of the Pho84 protein is regulated by factors other than properties of this sequence.

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Contribution to journal
publication status
published
keywords
Amino Acid Sequence, Base Sequence, Fungal Proteins, Gene Expression Regulation, Fungal, Green Fluorescent Proteins, Kinetics, Luminescent Proteins, Molecular Sequence Data, Mutagenesis, Site-Directed, Proton-Phosphate Symporters, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Journal Article, Research Support, Non-U.S. Gov't
in
FEBS Letters
volume
526
issue
1-3
pages
7 pages
publisher
Wiley-Blackwell
external identifiers
  • scopus:0037189949
ISSN
0014-5793
DOI
10.1016/S0014-5793(02)03109-5
language
English
LU publication?
no
id
9427aafb-ec67-4109-8134-49801175e7c7
date added to LUP
2017-10-19 20:12:45
date last changed
2017-11-06 14:12:01
@article{9427aafb-ec67-4109-8134-49801175e7c7,
  abstract     = {<p>A widely accepted mechanism for selective degradation of plasma membrane proteins is via ubiquitination and/or phosphorylation events. Such a regulated degradation has previously been suggested to rely on the presence of a specific SINNDAKSS sequence within the protein. Modification of a partly conserved SINNDAKSS-like sequence in the C-terminal tail of the Pho84 phosphate transporter, in combination with C-terminal fusion of green fluorescent protein or a MYC epitope, were used to evaluate the presence of this sequence and its role in the regulated degradation. The functional Pho84 mutants in which this SINNDAKSS-like sequence was altered or truncated were subjected to degradation like that of the wild type, suggesting that degradation of the Pho84 protein is regulated by factors other than properties of this sequence.</p>},
  author       = {Lagerstedt, Jens O. and Zvyagilskaya, Renata and Pratt, James R and Pattison-Granberg, Johanna and Kruckeberg, Arthur L and Berden, Jan A and Persson, Bengt L},
  issn         = {0014-5793},
  keyword      = {Amino Acid Sequence,Base Sequence,Fungal Proteins,Gene Expression Regulation, Fungal,Green Fluorescent Proteins,Kinetics,Luminescent Proteins,Molecular Sequence Data,Mutagenesis, Site-Directed,Proton-Phosphate Symporters,Recombinant Fusion Proteins,Saccharomyces cerevisiae,Saccharomyces cerevisiae Proteins,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  month        = {08},
  number       = {1-3},
  pages        = {7--31},
  publisher    = {Wiley-Blackwell},
  series       = {FEBS Letters},
  title        = {Mutagenic and functional analysis of the C-terminus of Saccharomyces cerevisiae Pho84 phosphate transporter},
  url          = {http://dx.doi.org/10.1016/S0014-5793(02)03109-5},
  volume       = {526},
  year         = {2002},
}