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The Mitochondrial DNA (mtDNA)-Associated Protein SWIB5 Influences mtDNA Architecture and Homologous Recombination

Blomme, Jonas ; Van Aken, Olivier LU ; Van Leene, Jelle ; Jégu, Teddy ; De Rycke, Riet Maria ; De Bruyne, Michiel ; Vercruysse, Jasmien ; Nolf, Jonah ; Van Daele, Twiggy and De Milde, Liesbeth , et al. (2017) In Plant Cell 29(5). p.1137-1156
Abstract

In addition to the nucleus, mitochondria and chloroplasts in plant cells also contain genomes. Efficient DNA repair pathways are crucial in these organelles to fix damage resulting from endogenous and exogenous factors. Plant organellar genomes are complex compared to their animal counterparts and although several plant-specific mediators of organelle DNA repair have been reported, many regulators remain to be identified. Here, we show that a mitochondrial SWI/SNF (nucleosome remodeling) complex B protein, SWIB5, is capable of associating with mitochondrial DNA (mtDNA) in Arabidopsis thaliana. Gain- and loss-of-function mutants provided evidence for a role of SWIB5 in influencing mtDNA architecture and homologous recombination at... (More)

In addition to the nucleus, mitochondria and chloroplasts in plant cells also contain genomes. Efficient DNA repair pathways are crucial in these organelles to fix damage resulting from endogenous and exogenous factors. Plant organellar genomes are complex compared to their animal counterparts and although several plant-specific mediators of organelle DNA repair have been reported, many regulators remain to be identified. Here, we show that a mitochondrial SWI/SNF (nucleosome remodeling) complex B protein, SWIB5, is capable of associating with mitochondrial DNA (mtDNA) in Arabidopsis thaliana. Gain- and loss-of-function mutants provided evidence for a role of SWIB5 in influencing mtDNA architecture and homologous recombination at specific intermediate-sized repeats both under normal and genotoxic conditions. SWIB5 interacts with other mitochondrial SWIB proteins. Gene expression and mutant phenotypic analysis of SWIB5 and SWIB family members suggests a link between organellar genome maintenance and cell proliferation. Taken together, our work presents a protein family that influences mtDNA architecture and homologous recombination in plants and suggests a link between organelle functioning and plant development.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Plant Cell
volume
29
issue
5
pages
20 pages
publisher
American Society of Plant Biologists
external identifiers
  • scopus:85020478590
  • wos:000402860900016
  • pmid:28420746
ISSN
1040-4651
DOI
10.1105/tpc.16.00899
language
English
LU publication?
yes
id
943c5059-f6ec-429b-a2f1-6c48a88cf343
date added to LUP
2017-05-08 10:41:44
date last changed
2025-01-07 12:40:06
@article{943c5059-f6ec-429b-a2f1-6c48a88cf343,
  abstract     = {{<p>In addition to the nucleus, mitochondria and chloroplasts in plant cells also contain genomes. Efficient DNA repair pathways are crucial in these organelles to fix damage resulting from endogenous and exogenous factors. Plant organellar genomes are complex compared to their animal counterparts and although several plant-specific mediators of organelle DNA repair have been reported, many regulators remain to be identified. Here, we show that a mitochondrial SWI/SNF (nucleosome remodeling) complex B protein, SWIB5, is capable of associating with mitochondrial DNA (mtDNA) in Arabidopsis thaliana. Gain- and loss-of-function mutants provided evidence for a role of SWIB5 in influencing mtDNA architecture and homologous recombination at specific intermediate-sized repeats both under normal and genotoxic conditions. SWIB5 interacts with other mitochondrial SWIB proteins. Gene expression and mutant phenotypic analysis of SWIB5 and SWIB family members suggests a link between organellar genome maintenance and cell proliferation. Taken together, our work presents a protein family that influences mtDNA architecture and homologous recombination in plants and suggests a link between organelle functioning and plant development.</p>}},
  author       = {{Blomme, Jonas and Van Aken, Olivier and Van Leene, Jelle and Jégu, Teddy and De Rycke, Riet Maria and De Bruyne, Michiel and Vercruysse, Jasmien and Nolf, Jonah and Van Daele, Twiggy and De Milde, Liesbeth and Vermeersch, Mattias and Colas des Francs-Small, Catherine and De Jaeger, Geert and Benhamed, Moussa and Millar, A. Harvey and Inzé, Dirk and Gonzalez, Nathalie}},
  issn         = {{1040-4651}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{1137--1156}},
  publisher    = {{American Society of Plant Biologists}},
  series       = {{Plant Cell}},
  title        = {{The Mitochondrial DNA (mtDNA)-Associated Protein SWIB5 Influences mtDNA Architecture and Homologous Recombination}},
  url          = {{http://dx.doi.org/10.1105/tpc.16.00899}},
  doi          = {{10.1105/tpc.16.00899}},
  volume       = {{29}},
  year         = {{2017}},
}