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Iron superoxide dismutase from the archaeon Sulfolobus sulfataricus : Analysis of structure and thermostability.

Ursby, Thomas LU ; Adinolfi, Bianca Stella; Al-Karadaghi, Salam LU ; De Vendittis, Emmanuele and Buccini, Vincenzo (1999) In Journal of Molecular Biology 286(1). p.189-205
Abstract
The crystal structure of superoxide dismutase (SOD) from the hyper thermophile Sulfolobus solfataricus has been determined at 2.3 Å resolution by molecular replacement and refined to a crystallographic R-factor of 16.8 % (Rfree 19.8 %). The crystals belong to the space group C2 (a = 76.3 Å, b = 124.3 Å,c = 60.3 Å, β = 128.8°) with two identical monomers in the asymmetric unit. The monomer has a molecular weight of 24 kDa and consists of 210 amino acid residues of which 205 are visible in the electron density map. The overall fold of the monomer of S. solfataricus SOD is similar to that of the other known Fe or Mn-SODs. S. solfataricus SOD forms a very compact tetramer of a type similar to that of SOD from the hyperthermophile Aquifex... (More)
The crystal structure of superoxide dismutase (SOD) from the hyper thermophile Sulfolobus solfataricus has been determined at 2.3 Å resolution by molecular replacement and refined to a crystallographic R-factor of 16.8 % (Rfree 19.8 %). The crystals belong to the space group C2 (a = 76.3 Å, b = 124.3 Å,c = 60.3 Å, β = 128.8°) with two identical monomers in the asymmetric unit. The monomer has a molecular weight of 24 kDa and consists of 210 amino acid residues of which 205 are visible in the electron density map. The overall fold of the monomer of S. solfataricus SOD is similar to that of the other known Fe or Mn-SODs. S. solfataricus SOD forms a very compact tetramer of a type similar to that of SOD from the hyperthermophile Aquifex pyrophilus. Both structures show an elevated number of inter-subunit ion-pairs compared with the mesophilic SOD from Mycobacterium tuberculosis and the thermophilic SOD from Thermus thermophilus. However, in contrast to the A. pyrophilus SOD structure, the number of intra-subunit ion-pairs as well as inter-subunit hydrogen bonds is not higher than in the compared mesophilic and thermophilic SOD structures. The electron density also revealed an unexpected and unusual covalent modification of a conserved tyrosine in the active site. Its involvement in the specific activity of the enzyme is discussed. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
superoxide dismutase, Sulfolobus solfataricus, X-ray structure, thermostability, tyrosine modification
in
Journal of Molecular Biology
volume
286
issue
1
pages
189 - 205
publisher
Elsevier
external identifiers
  • scopus:0033548190
ISSN
1089-8638
DOI
10.1006/jmbi.1998.2471
language
English
LU publication?
yes
id
dfec1ab7-c345-41a2-aa7e-bc38a2c6ce8a (old id 948393)
date added to LUP
2008-07-17 11:02:04
date last changed
2017-08-06 04:36:35
@article{dfec1ab7-c345-41a2-aa7e-bc38a2c6ce8a,
  abstract     = {The crystal structure of superoxide dismutase (SOD) from the hyper thermophile Sulfolobus solfataricus has been determined at 2.3 Å resolution by molecular replacement and refined to a crystallographic R-factor of 16.8 % (Rfree 19.8 %). The crystals belong to the space group C2 (a = 76.3 Å, b = 124.3 Å,c = 60.3 Å, β = 128.8°) with two identical monomers in the asymmetric unit. The monomer has a molecular weight of 24 kDa and consists of 210 amino acid residues of which 205 are visible in the electron density map. The overall fold of the monomer of S. solfataricus SOD is similar to that of the other known Fe or Mn-SODs. S. solfataricus SOD forms a very compact tetramer of a type similar to that of SOD from the hyperthermophile Aquifex pyrophilus. Both structures show an elevated number of inter-subunit ion-pairs compared with the mesophilic SOD from Mycobacterium tuberculosis and the thermophilic SOD from Thermus thermophilus. However, in contrast to the A. pyrophilus SOD structure, the number of intra-subunit ion-pairs as well as inter-subunit hydrogen bonds is not higher than in the compared mesophilic and thermophilic SOD structures. The electron density also revealed an unexpected and unusual covalent modification of a conserved tyrosine in the active site. Its involvement in the specific activity of the enzyme is discussed.},
  author       = {Ursby, Thomas and Adinolfi, Bianca Stella and Al-Karadaghi, Salam and De Vendittis, Emmanuele and Buccini, Vincenzo},
  issn         = {1089-8638},
  keyword      = {superoxide dismutase,Sulfolobus solfataricus,X-ray structure,thermostability,tyrosine modification},
  language     = {eng},
  number       = {1},
  pages        = {189--205},
  publisher    = {Elsevier},
  series       = {Journal of Molecular Biology},
  title        = {Iron superoxide dismutase from the archaeon Sulfolobus sulfataricus : Analysis of structure and thermostability.},
  url          = {http://dx.doi.org/10.1006/jmbi.1998.2471},
  volume       = {286},
  year         = {1999},
}