Label-free quantitative proteomics reveals differentially regulated proteins in experimental gingivitis
(2013) In Journal of Proteome Research 12(2). p.78-657- Abstract
We investigated the sequential protein expression in gingival crevicular fluid samples during the induction (I) and resolution (R) of experimental gingivitis. Periodontally and systemically healthy volunteers (n = 20) participated in a three-week experimental gingivitis protocol, followed by debridement and two weeks of regular plaque control. Gingival crevicular fluid (GCF) samples were collected at baseline, Day 7, 14, and 21 (induction; I-phase), and at Day 21, 25, 30, and 35 (resolution; R-phase). Liquid chromatography-tandem mass spectrometry (LC-MS/MS) for label-free quantitative proteomics was applied. A total of 287 proteins were identified including 254 human, 14 bacterial, 12 fungal, and 7 yeast proteins. Ontology analysis... (More)
We investigated the sequential protein expression in gingival crevicular fluid samples during the induction (I) and resolution (R) of experimental gingivitis. Periodontally and systemically healthy volunteers (n = 20) participated in a three-week experimental gingivitis protocol, followed by debridement and two weeks of regular plaque control. Gingival crevicular fluid (GCF) samples were collected at baseline, Day 7, 14, and 21 (induction; I-phase), and at Day 21, 25, 30, and 35 (resolution; R-phase). Liquid chromatography-tandem mass spectrometry (LC-MS/MS) for label-free quantitative proteomics was applied. A total of 287 proteins were identified including 254 human, 14 bacterial, 12 fungal, and 7 yeast proteins. Ontology analysis revealed proteins primarily involved in cytoskeletal rearrangements, immune response, antimicrobial function, protein degradation, and DNA binding. There was considerable variation in the number of proteins identified, both among subjects and within subjects across time points. After pooling of samples between subjects at each time point, the levels of 59 proteins in the I-phase and 73 proteins in the R-phase were quantified longitudinally. Our data demonstrate that LC-MS/MS label-free quantitative proteomics is valuable in the assessment of the protein content of the GCF and can facilitate a better understanding of the molecular mechanisms involved in the induction and resolution of plaque-induced gingival inflammation in humans.
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- author
- Bostanci, Nagihan ; Ramberg, Per ; Wahlander, Åsa ; Grossman, Jonas ; Jönsson, Daniel LU ; Barnes, Virginia Monsul and Papapanou, Panos N
- publishing date
- 2013-02-01
- type
- Contribution to journal
- publication status
- published
- keywords
- Adult, Bacterial Proteins/analysis, Chromatography, Liquid, Female, Fungal Proteins/analysis, Gene Expression Regulation, Gingival Crevicular Fluid/chemistry, Gingivitis/genetics, Humans, Male, Proteome/analysis, Salivary Proteins and Peptides/analysis, Tandem Mass Spectrometry
- in
- Journal of Proteome Research
- volume
- 12
- issue
- 2
- pages
- 78 - 657
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- scopus:84873338251
- pmid:23244068
- ISSN
- 1535-3893
- DOI
- 10.1021/pr300761e
- language
- English
- LU publication?
- no
- id
- 94bf4f5b-efbc-47d2-9cec-375e6d09a2bf
- date added to LUP
- 2024-07-04 11:03:30
- date last changed
- 2025-04-12 09:06:43
@article{94bf4f5b-efbc-47d2-9cec-375e6d09a2bf,
abstract = {{<p>We investigated the sequential protein expression in gingival crevicular fluid samples during the induction (I) and resolution (R) of experimental gingivitis. Periodontally and systemically healthy volunteers (n = 20) participated in a three-week experimental gingivitis protocol, followed by debridement and two weeks of regular plaque control. Gingival crevicular fluid (GCF) samples were collected at baseline, Day 7, 14, and 21 (induction; I-phase), and at Day 21, 25, 30, and 35 (resolution; R-phase). Liquid chromatography-tandem mass spectrometry (LC-MS/MS) for label-free quantitative proteomics was applied. A total of 287 proteins were identified including 254 human, 14 bacterial, 12 fungal, and 7 yeast proteins. Ontology analysis revealed proteins primarily involved in cytoskeletal rearrangements, immune response, antimicrobial function, protein degradation, and DNA binding. There was considerable variation in the number of proteins identified, both among subjects and within subjects across time points. After pooling of samples between subjects at each time point, the levels of 59 proteins in the I-phase and 73 proteins in the R-phase were quantified longitudinally. Our data demonstrate that LC-MS/MS label-free quantitative proteomics is valuable in the assessment of the protein content of the GCF and can facilitate a better understanding of the molecular mechanisms involved in the induction and resolution of plaque-induced gingival inflammation in humans.</p>}},
author = {{Bostanci, Nagihan and Ramberg, Per and Wahlander, Åsa and Grossman, Jonas and Jönsson, Daniel and Barnes, Virginia Monsul and Papapanou, Panos N}},
issn = {{1535-3893}},
keywords = {{Adult; Bacterial Proteins/analysis; Chromatography, Liquid; Female; Fungal Proteins/analysis; Gene Expression Regulation; Gingival Crevicular Fluid/chemistry; Gingivitis/genetics; Humans; Male; Proteome/analysis; Salivary Proteins and Peptides/analysis; Tandem Mass Spectrometry}},
language = {{eng}},
month = {{02}},
number = {{2}},
pages = {{78--657}},
publisher = {{The American Chemical Society (ACS)}},
series = {{Journal of Proteome Research}},
title = {{Label-free quantitative proteomics reveals differentially regulated proteins in experimental gingivitis}},
url = {{http://dx.doi.org/10.1021/pr300761e}},
doi = {{10.1021/pr300761e}},
volume = {{12}},
year = {{2013}},
}