Advanced

Survival and functional recovery of transplanted human dopaminergic neurons into hemiparkinsonian rats depend on the cannula size of the implantation instrument

Steiner, Barbara; Winter, Christine; Blumensath, Stefan; Paul, Gesine LU ; Harnack, Daniel; Nikkhah, Guido and Kupsch, Andreas (2008) In Journal of Neuroscience Methods 169(1). p.128-134
Abstract

Promising therapeutic strategies for neurodegenerative diseases such as Parkinson's disease include replacement of lost striatal dopaminergic neurons by grafting of embryonic mesencephalic cells. However, the poor survival of the transplanted tissue still limits transplantation of these cells into the human brain in a larger number of patients. We addressed the question, if the diameter of the transplantation cannulas has an effect on the number of surviving transplanted human embryonic mesencephalic cells into the striatum of 6-OHDA lesioned rats. We report a significantly higher number of surviving human cells using an ultrathin micropipette compared to cannulas with wider diameters. Importantly, higher numbers of surviving cells also... (More)

Promising therapeutic strategies for neurodegenerative diseases such as Parkinson's disease include replacement of lost striatal dopaminergic neurons by grafting of embryonic mesencephalic cells. However, the poor survival of the transplanted tissue still limits transplantation of these cells into the human brain in a larger number of patients. We addressed the question, if the diameter of the transplantation cannulas has an effect on the number of surviving transplanted human embryonic mesencephalic cells into the striatum of 6-OHDA lesioned rats. We report a significantly higher number of surviving human cells using an ultrathin micropipette compared to cannulas with wider diameters. Importantly, higher numbers of surviving cells also correlated with a behavioral recovery of the hemiparkinsonian rats.

(Less)
Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
published
keywords
Cannula size, Human dopaminergic cells, Transplantation
in
Journal of Neuroscience Methods
volume
169
issue
1
pages
7 pages
publisher
Elsevier
external identifiers
  • scopus:39749112525
ISSN
0165-0270
DOI
10.1016/j.jneumeth.2007.11.032
language
English
LU publication?
no
id
94d4cacf-ba28-41f7-99c4-ac64dd18a0b6
date added to LUP
2017-05-18 12:49:47
date last changed
2017-11-05 05:17:34
@article{94d4cacf-ba28-41f7-99c4-ac64dd18a0b6,
  abstract     = {<p>Promising therapeutic strategies for neurodegenerative diseases such as Parkinson's disease include replacement of lost striatal dopaminergic neurons by grafting of embryonic mesencephalic cells. However, the poor survival of the transplanted tissue still limits transplantation of these cells into the human brain in a larger number of patients. We addressed the question, if the diameter of the transplantation cannulas has an effect on the number of surviving transplanted human embryonic mesencephalic cells into the striatum of 6-OHDA lesioned rats. We report a significantly higher number of surviving human cells using an ultrathin micropipette compared to cannulas with wider diameters. Importantly, higher numbers of surviving cells also correlated with a behavioral recovery of the hemiparkinsonian rats.</p>},
  author       = {Steiner, Barbara and Winter, Christine and Blumensath, Stefan and Paul, Gesine and Harnack, Daniel and Nikkhah, Guido and Kupsch, Andreas},
  issn         = {0165-0270},
  keyword      = {Cannula size,Human dopaminergic cells,Transplantation},
  language     = {eng},
  month        = {03},
  number       = {1},
  pages        = {128--134},
  publisher    = {Elsevier},
  series       = {Journal of Neuroscience Methods},
  title        = {Survival and functional recovery of transplanted human dopaminergic neurons into hemiparkinsonian rats depend on the cannula size of the implantation instrument},
  url          = {http://dx.doi.org/10.1016/j.jneumeth.2007.11.032},
  volume       = {169},
  year         = {2008},
}