Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Spatio-temporal evolution of hydroxyapatite crystal thickness at the bone-implant interface

Cann, Sophie Le LU ; Törnquist, Elin LU ; Barreto, Isabella Silva LU orcid ; Fraulob, Manon ; Lomami, Hugues Albini ; Verezhak, Mariana ; Guizar-Sicairos, Manuel ; Isaksson, Hanna LU orcid and Haïat, Guillaume (2020) In Acta Biomaterialia 116. p.391-399
Abstract

A better understanding of bone nanostructure around the bone-implant interface is essential to improve longevity of clinical implants and decrease failure risks. This study investigates the spatio-temporal evolution of mineral crystal thickness and plate orientation in newly formed bone around the surface of a metallic implant. Standardized coin-shaped titanium implants designed with a bone chamber were inserted into rabbit tibiae for 7 and 13 weeks. Scanning measurements with micro-focused small-angle X-ray scattering (SAXS) were carried out on newly formed bone close to the implant and in control mature cortical bone. Mineral crystals were thinner close to the implant (1.8 ± 0.45 nm at 7 weeks and 2.4 ± 0.57 nm at 13 weeks) than in... (More)

A better understanding of bone nanostructure around the bone-implant interface is essential to improve longevity of clinical implants and decrease failure risks. This study investigates the spatio-temporal evolution of mineral crystal thickness and plate orientation in newly formed bone around the surface of a metallic implant. Standardized coin-shaped titanium implants designed with a bone chamber were inserted into rabbit tibiae for 7 and 13 weeks. Scanning measurements with micro-focused small-angle X-ray scattering (SAXS) were carried out on newly formed bone close to the implant and in control mature cortical bone. Mineral crystals were thinner close to the implant (1.8 ± 0.45 nm at 7 weeks and 2.4 ± 0.57 nm at 13 weeks) than in the control mature bone tissue (2.5 ± 0.21 nm at 7 weeks and 2.8 ± 0.35 nm at 13 weeks), with increasing thickness over healing time (+30 % in 6 weeks). These results are explained by younger bone close to the implant, which matures during osseointegration. Thinner mineral crystals parallel to the implant surface within the first 100 µm close to the implant indicate that the implant affects bone ultrastructure close to the implant, potentially due to heterogeneous interfacial stresses, and suggest a longer maturation process of bone tissue and difficulty in binding to the metal. The bone growth kinetics within the bone chamber was derived from the spatio-temporal evolution of bone tissue's nanostructure, coupled with microtomographic imaging. The findings indicate that understanding mineral crystal thickness or plate orientation can improve our knowledge of osseointegration.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Bone-implant interface, Small-angle X-ray scattering, Osseointegration, Hydroxyapatite
in
Acta Biomaterialia
volume
116
pages
9 pages
publisher
Elsevier
external identifiers
  • scopus:85091517810
  • pmid:32937205
ISSN
1878-7568
DOI
10.1016/j.actbio.2020.09.021
language
English
LU publication?
yes
additional info
Copyright © 2020. Published by Elsevier Ltd.
id
952cd6ac-b10e-401a-92e1-bb039a312ce3
date added to LUP
2020-09-22 10:44:33
date last changed
2024-05-01 16:41:43
@article{952cd6ac-b10e-401a-92e1-bb039a312ce3,
  abstract     = {{<p>A better understanding of bone nanostructure around the bone-implant interface is essential to improve longevity of clinical implants and decrease failure risks. This study investigates the spatio-temporal evolution of mineral crystal thickness and plate orientation in newly formed bone around the surface of a metallic implant. Standardized coin-shaped titanium implants designed with a bone chamber were inserted into rabbit tibiae for 7 and 13 weeks. Scanning measurements with micro-focused small-angle X-ray scattering (SAXS) were carried out on newly formed bone close to the implant and in control mature cortical bone. Mineral crystals were thinner close to the implant (1.8 ± 0.45 nm at 7 weeks and 2.4 ± 0.57 nm at 13 weeks) than in the control mature bone tissue (2.5 ± 0.21 nm at 7 weeks and 2.8 ± 0.35 nm at 13 weeks), with increasing thickness over healing time (+30 % in 6 weeks). These results are explained by younger bone close to the implant, which matures during osseointegration. Thinner mineral crystals parallel to the implant surface within the first 100 µm close to the implant indicate that the implant affects bone ultrastructure close to the implant, potentially due to heterogeneous interfacial stresses, and suggest a longer maturation process of bone tissue and difficulty in binding to the metal. The bone growth kinetics within the bone chamber was derived from the spatio-temporal evolution of bone tissue's nanostructure, coupled with microtomographic imaging. The findings indicate that understanding mineral crystal thickness or plate orientation can improve our knowledge of osseointegration.</p>}},
  author       = {{Cann, Sophie Le and Törnquist, Elin and Barreto, Isabella Silva and Fraulob, Manon and Lomami, Hugues Albini and Verezhak, Mariana and Guizar-Sicairos, Manuel and Isaksson, Hanna and Haïat, Guillaume}},
  issn         = {{1878-7568}},
  keywords     = {{Bone-implant interface; Small-angle X-ray scattering; Osseointegration; Hydroxyapatite}},
  language     = {{eng}},
  month        = {{10}},
  pages        = {{391--399}},
  publisher    = {{Elsevier}},
  series       = {{Acta Biomaterialia}},
  title        = {{Spatio-temporal evolution of hydroxyapatite crystal thickness at the bone-implant interface}},
  url          = {{http://dx.doi.org/10.1016/j.actbio.2020.09.021}},
  doi          = {{10.1016/j.actbio.2020.09.021}},
  volume       = {{116}},
  year         = {{2020}},
}