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Comparison of histological delineations of medial temporal lobe cortices by four independent neuroanatomy laboratories

Wuestefeld, Anika LU orcid ; Baumeister, Hannah ; Adams, Jenna N ; de Flores, Robin ; Hodgetts, Carl ; Mazloum-Farzaghi, Negar ; Olsen, Rosanna K ; Puliyadi, Vyash ; Tran, Tammy T and Bakker, Arnold , et al. (2023)
Abstract

The medial temporal lobe (MTL) cortex, located adjacent to the hippocampus, is crucial for memory and prone to the accumulation of certain neuropathologies such as Alzheimer's disease neurofibrillary tau tangles. The MTL cortex is composed of several subregions which differ in their functional and cytoarchitectonic features. As neuroanatomical schools rely on different cytoarchitectonic definitions of these subregions, it is unclear to what extent their delineations of MTL cortex subregions overlap. Here, we provide an overview of cytoarchitectonic definitions of the cortices that make up the parahippocampal gyrus (entorhinal and parahippocampal cortices) and the adjacent Brodmann areas (BA) 35 and 36, as provided by four... (More)

The medial temporal lobe (MTL) cortex, located adjacent to the hippocampus, is crucial for memory and prone to the accumulation of certain neuropathologies such as Alzheimer's disease neurofibrillary tau tangles. The MTL cortex is composed of several subregions which differ in their functional and cytoarchitectonic features. As neuroanatomical schools rely on different cytoarchitectonic definitions of these subregions, it is unclear to what extent their delineations of MTL cortex subregions overlap. Here, we provide an overview of cytoarchitectonic definitions of the cortices that make up the parahippocampal gyrus (entorhinal and parahippocampal cortices) and the adjacent Brodmann areas (BA) 35 and 36, as provided by four neuroanatomists from different laboratories, aiming to identify the rationale for overlapping and diverging delineations. Nissl-stained series were acquired from the temporal lobes of three human specimens (two right and one left hemisphere). Slices (50 µm thick) were prepared perpendicular to the long axis of the hippocampus spanning the entire longitudinal extent of the MTL cortex. Four neuroanatomists annotated MTL cortex subregions on digitized (20X resolution) slices with 5 mm spacing. Parcellations, terminology, and border placement were compared among neuroanatomists. Cytoarchitectonic features of each subregion are described in detail. Qualitative analysis of the annotations showed higher agreement in the definitions of the entorhinal cortex and BA35, while definitions of BA36 and the parahippocampal cortex exhibited less overlap among neuroanatomists. The degree of overlap of cytoarchitectonic definitions was partially reflected in the neuroanatomists' agreement on the respective delineations. Lower agreement in annotations was observed in transitional zones between structures where seminal cytoarchitectonic features are expressed more gradually. The results highlight that definitions and parcellations of the MTL cortex differ among neuroanatomical schools and thereby increase understanding of why these differences may arise. This work sets a crucial foundation to further advance anatomically-informed human neuroimaging research on the MTL cortex.

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organization
publishing date
type
Working paper/Preprint
publication status
published
subject
publisher
bioRxiv
external identifiers
  • pmid:37292729
DOI
10.1101/2023.05.24.542054
language
English
LU publication?
yes
id
9556e3ea-d8ee-4fcc-9631-867fa77ad1f4
date added to LUP
2023-06-14 09:47:02
date last changed
2023-06-15 03:07:46
@misc{9556e3ea-d8ee-4fcc-9631-867fa77ad1f4,
  abstract     = {{<p>The medial temporal lobe (MTL) cortex, located adjacent to the hippocampus, is crucial for memory and prone to the accumulation of certain neuropathologies such as Alzheimer's disease neurofibrillary tau tangles. The MTL cortex is composed of several subregions which differ in their functional and cytoarchitectonic features. As neuroanatomical schools rely on different cytoarchitectonic definitions of these subregions, it is unclear to what extent their delineations of MTL cortex subregions overlap. Here, we provide an overview of cytoarchitectonic definitions of the cortices that make up the parahippocampal gyrus (entorhinal and parahippocampal cortices) and the adjacent Brodmann areas (BA) 35 and 36, as provided by four neuroanatomists from different laboratories, aiming to identify the rationale for overlapping and diverging delineations. Nissl-stained series were acquired from the temporal lobes of three human specimens (two right and one left hemisphere). Slices (50 µm thick) were prepared perpendicular to the long axis of the hippocampus spanning the entire longitudinal extent of the MTL cortex. Four neuroanatomists annotated MTL cortex subregions on digitized (20X resolution) slices with 5 mm spacing. Parcellations, terminology, and border placement were compared among neuroanatomists. Cytoarchitectonic features of each subregion are described in detail. Qualitative analysis of the annotations showed higher agreement in the definitions of the entorhinal cortex and BA35, while definitions of BA36 and the parahippocampal cortex exhibited less overlap among neuroanatomists. The degree of overlap of cytoarchitectonic definitions was partially reflected in the neuroanatomists' agreement on the respective delineations. Lower agreement in annotations was observed in transitional zones between structures where seminal cytoarchitectonic features are expressed more gradually. The results highlight that definitions and parcellations of the MTL cortex differ among neuroanatomical schools and thereby increase understanding of why these differences may arise. This work sets a crucial foundation to further advance anatomically-informed human neuroimaging research on the MTL cortex.</p>}},
  author       = {{Wuestefeld, Anika and Baumeister, Hannah and Adams, Jenna N and de Flores, Robin and Hodgetts, Carl and Mazloum-Farzaghi, Negar and Olsen, Rosanna K and Puliyadi, Vyash and Tran, Tammy T and Bakker, Arnold and Canada, Kelsey L and Dalton, Marshall A and Daugherty, Ana M and Joie, Renaud La and Wang, Lei and Bedard, Madigan and Buendia, Esther and Denning, Amanda and Irwin, David J and Ittyerah, Ranjit and Lee, Edward B and Lim, Sydney and de Onzoño Martin, Maria Mercedes Iñiguez and Lopez, Monica Munoz and Schuck, Theresa and Trotman, Winifred and Vela, Alicia and Yushkevich, Paul and Amunts, Katrin and Augustinack, Jean C and Ding, Song-Lin and Insausti, Ricardo and Kedo, Olga and Berron, David and Wisse, Laura E M}},
  language     = {{eng}},
  month        = {{05}},
  note         = {{Preprint}},
  publisher    = {{bioRxiv}},
  title        = {{Comparison of histological delineations of medial temporal lobe cortices by four independent neuroanatomy laboratories}},
  url          = {{http://dx.doi.org/10.1101/2023.05.24.542054}},
  doi          = {{10.1101/2023.05.24.542054}},
  year         = {{2023}},
}