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Histomorphometric and osteocytic characteristics of cortical bone in male subtrochanteric femoral shaft

Tong, Xiaoyu; Malo, Markus K.H.; Burton, Inari S.; Jurvelin, Jukka S.; Isaksson, Hanna LU and Kröger, Heikki (2017) In Journal of Anatomy 231(5). p.708-717
Abstract

The histomorphometric properties of the subtrochanteric femoral region have rarely been investigated. The aim of this study was to investigate the age-associated variations and regional differences of histomorphometric and osteocytic properties in the cortical bone of the subtrochanteric femoral shaft, and the association between osteocytic and histological cortical bone parameters. Undecalcified histological sections of the subtrochanteric femoral shaft were obtained from cadavers (n = 20, aged 18–82 years, males). They were cut and stained using modified Masson-Goldner stain. Histomorphometric parameters of cortical bone were analysed with ×50 and ×100 magnification after identifying cortical bone boundaries using our previously... (More)

The histomorphometric properties of the subtrochanteric femoral region have rarely been investigated. The aim of this study was to investigate the age-associated variations and regional differences of histomorphometric and osteocytic properties in the cortical bone of the subtrochanteric femoral shaft, and the association between osteocytic and histological cortical bone parameters. Undecalcified histological sections of the subtrochanteric femoral shaft were obtained from cadavers (n = 20, aged 18–82 years, males). They were cut and stained using modified Masson-Goldner stain. Histomorphometric parameters of cortical bone were analysed with ×50 and ×100 magnification after identifying cortical bone boundaries using our previously validated method. Within cortical bone areas, only complete osteons with typical concentric lamellae and cement line were selected and measured. Osteocytic parameters of cortical bone were analyzed under phase contrast microscopy and epifluorescence within microscopic fields (0.55 mm2 for each). The cortical widths of the medial and lateral quadrants were significantly higher than other quadrants (P < 0.01). Osteonal area per cortical bone area was lower and cortical porosities were higher in the posterior quadrant than in the other quadrants (P < 0.05). Osteocyte lacunar number per cortical bone area was found higher in the young subjects (≤ 50 years) than in the older ones (> 50 years) both before and after adjustments for body height and weight (P < 0.05). Moreover, significant but low correlations were found between the cortical bone and osteocytic parameters (0.20 ≤ R2 ≤ 0.35, P < 0.05). It can be concluded that in healthy males, the cortical histomorphometric parameters differ between the anatomical regions of the subtrochanteric femoral shaft, and are correlated with the osteocytic parameters from the same site. These findings may be of use when discussing mechanisms that predispose patients to decreasing bone strength.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
bone histomorphometry, cortical bone, osteocyte, regional difference, subtrochanteric femoral shaft
in
Journal of Anatomy
volume
231
issue
5
pages
10 pages
publisher
John Wiley & Sons
external identifiers
  • scopus:85035199393
ISSN
0021-8782
DOI
10.1111/joa.12670
language
English
LU publication?
yes
id
95995b7b-9ccf-4c08-a6f7-c02dd508c9b4
date added to LUP
2018-01-31 09:19:36
date last changed
2019-06-25 03:29:09
@article{95995b7b-9ccf-4c08-a6f7-c02dd508c9b4,
  abstract     = {<p>The histomorphometric properties of the subtrochanteric femoral region have rarely been investigated. The aim of this study was to investigate the age-associated variations and regional differences of histomorphometric and osteocytic properties in the cortical bone of the subtrochanteric femoral shaft, and the association between osteocytic and histological cortical bone parameters. Undecalcified histological sections of the subtrochanteric femoral shaft were obtained from cadavers (n = 20, aged 18–82 years, males). They were cut and stained using modified Masson-Goldner stain. Histomorphometric parameters of cortical bone were analysed with ×50 and ×100 magnification after identifying cortical bone boundaries using our previously validated method. Within cortical bone areas, only complete osteons with typical concentric lamellae and cement line were selected and measured. Osteocytic parameters of cortical bone were analyzed under phase contrast microscopy and epifluorescence within microscopic fields (0.55 mm<sup>2</sup> for each). The cortical widths of the medial and lateral quadrants were significantly higher than other quadrants (P &lt; 0.01). Osteonal area per cortical bone area was lower and cortical porosities were higher in the posterior quadrant than in the other quadrants (P &lt; 0.05). Osteocyte lacunar number per cortical bone area was found higher in the young subjects (≤ 50 years) than in the older ones (&gt; 50 years) both before and after adjustments for body height and weight (P &lt; 0.05). Moreover, significant but low correlations were found between the cortical bone and osteocytic parameters (0.20 ≤ R<sup>2</sup> ≤ 0.35, P &lt; 0.05). It can be concluded that in healthy males, the cortical histomorphometric parameters differ between the anatomical regions of the subtrochanteric femoral shaft, and are correlated with the osteocytic parameters from the same site. These findings may be of use when discussing mechanisms that predispose patients to decreasing bone strength.</p>},
  author       = {Tong, Xiaoyu and Malo, Markus K.H. and Burton, Inari S. and Jurvelin, Jukka S. and Isaksson, Hanna and Kröger, Heikki},
  issn         = {0021-8782},
  keyword      = {bone histomorphometry,cortical bone,osteocyte,regional difference,subtrochanteric femoral shaft},
  language     = {eng},
  number       = {5},
  pages        = {708--717},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Anatomy},
  title        = {Histomorphometric and osteocytic characteristics of cortical bone in male subtrochanteric femoral shaft},
  url          = {http://dx.doi.org/10.1111/joa.12670},
  volume       = {231},
  year         = {2017},
}