Serum exchange and use of dilutions have improved precision of measurement of islet cell antibodies
Bonifacio, E. ; Lernmark, A. LU
; Dawkins, R. L.
and co-authors
(1988)
In Journal of Immunological Methods
106(1).
p.83-88
- Abstract
In an attempt to improve the diagnostic value of measuring antibodies to islet cell cytoplasmic antigen, coded sera were distributed to 38 laboratories and results were returned for analysis. Comparison between laboratories revealed that results for some individual sera differed by up nine doubling dilutions and even within laboratories duplicate samples could differ by six doubling dilutions. By including dilutions of sera it was possible to draw a standard curve for each laboratory and this revealed major variations in shape, slope and intercept. However, using each laboratory's standard curve and converting results to units, a substantial improvement was obtained. The approach described improves standardisation and will permit... (More)
In an attempt to improve the diagnostic value of measuring antibodies to islet cell cytoplasmic antigen, coded sera were distributed to 38 laboratories and results were returned for analysis. Comparison between laboratories revealed that results for some individual sera differed by up nine doubling dilutions and even within laboratories duplicate samples could differ by six doubling dilutions. By including dilutions of sera it was possible to draw a standard curve for each laboratory and this revealed major variations in shape, slope and intercept. However, using each laboratory's standard curve and converting results to units, a substantial improvement was obtained. The approach described improves standardisation and will permit laboratories to identify poor precision and/or any systematic change in assay performance.
(Less)
- author
- Bonifacio, E.
; Lernmark, A.
LU
; Dawkins, R. L.
and co-authors
- publishing date
- 1988-01-21
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Insulin-dependent diabetes mellitus, Islet cell antibody, Standards
- in
- Journal of Immunological Methods
- volume
- 106
- issue
- 1
- pages
- 6 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:3123555
- scopus:0023878282
- ISSN
- 0022-1759
- DOI
- 10.1016/0022-1759(88)90274-8
- language
- English
- LU publication?
- no
- id
- 95b78f66-c088-4757-af2f-dc3e19aaa9ac
- date added to LUP
- 2019-12-12 23:21:27
- date last changed
- 2024-03-13 08:25:44
@article{95b78f66-c088-4757-af2f-dc3e19aaa9ac,
abstract = {{<p>In an attempt to improve the diagnostic value of measuring antibodies to islet cell cytoplasmic antigen, coded sera were distributed to 38 laboratories and results were returned for analysis. Comparison between laboratories revealed that results for some individual sera differed by up nine doubling dilutions and even within laboratories duplicate samples could differ by six doubling dilutions. By including dilutions of sera it was possible to draw a standard curve for each laboratory and this revealed major variations in shape, slope and intercept. However, using each laboratory's standard curve and converting results to units, a substantial improvement was obtained. The approach described improves standardisation and will permit laboratories to identify poor precision and/or any systematic change in assay performance.</p>}},
author = {{Bonifacio, E. and Lernmark, A. and Dawkins, R. L. and co-authors}},
issn = {{0022-1759}},
keywords = {{Insulin-dependent diabetes mellitus; Islet cell antibody; Standards}},
language = {{eng}},
month = {{01}},
number = {{1}},
pages = {{83--88}},
publisher = {{Elsevier}},
series = {{Journal of Immunological Methods}},
title = {{Serum exchange and use of dilutions have improved precision of measurement of islet cell antibodies}},
url = {{http://dx.doi.org/10.1016/0022-1759(88)90274-8}},
doi = {{10.1016/0022-1759(88)90274-8}},
volume = {{106}},
year = {{1988}},
}