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Novel tools to quantify total, phospho-Ser129 and aggregated alpha-synuclein in the mouse brain

Trist, Benjamin Guy ; Wright, Courtney Jade LU ; Rangel, Alejandra ; Cottle, Louise ; Prasad, Asheeta ; Jensen, Nanna Møller ; Gram, Hjalte ; Dzamko, Nicolas ; Jensen, Poul Henning and Kirik, Deniz LU orcid (2024) In npj Parkinson's Disease 10(1).
Abstract

Assays for quantifying aggregated and phosphorylated (S129) human α-synuclein protein are widely used to evaluate pathological burden in patients suffering from synucleinopathy disorders. Many of these assays, however, do not cross-react with mouse α-synuclein or exhibit poor sensitivity for this target, which is problematic considering the preponderance of mouse models at the forefront of pre-clinical α-synuclein research. In this project, we addressed this unmet need by reformulating two existing AlphaLISA® SureFire® Ultra™ total and pS129 α-synuclein assay kits to yield robust and ultrasensitive (LLoQ ≤ 0.5 pg/mL) quantification of mouse and human wild-type and pS129 α-synuclein protein. We then employed these... (More)

Assays for quantifying aggregated and phosphorylated (S129) human α-synuclein protein are widely used to evaluate pathological burden in patients suffering from synucleinopathy disorders. Many of these assays, however, do not cross-react with mouse α-synuclein or exhibit poor sensitivity for this target, which is problematic considering the preponderance of mouse models at the forefront of pre-clinical α-synuclein research. In this project, we addressed this unmet need by reformulating two existing AlphaLISA® SureFire® Ultra™ total and pS129 α-synuclein assay kits to yield robust and ultrasensitive (LLoQ ≤ 0.5 pg/mL) quantification of mouse and human wild-type and pS129 α-synuclein protein. We then employed these assays, together with the BioLegend α-synuclein aggregate ELISA, to assess α-synuclein S129 phosphorylation and aggregation in different mouse brain tissue preparations. Overall, we highlight the compatibility of these new immunoassays with rodent models and demonstrate their potential to advance knowledge surrounding α-synuclein phosphorylation and aggregation in synucleinopathies.

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author
; ; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
npj Parkinson's Disease
volume
10
issue
1
article number
217
publisher
Springer Nature
external identifiers
  • pmid:39516469
  • scopus:85209807995
ISSN
2373-8057
DOI
10.1038/s41531-024-00830-y
language
English
LU publication?
yes
additional info
Publisher Copyright: © The Author(s) 2024.
id
95d24e8c-0b3c-480e-a7ed-2702f545e0a3
date added to LUP
2025-01-10 14:25:07
date last changed
2025-07-12 05:44:24
@article{95d24e8c-0b3c-480e-a7ed-2702f545e0a3,
  abstract     = {{<p>Assays for quantifying aggregated and phosphorylated (S129) human α-synuclein protein are widely used to evaluate pathological burden in patients suffering from synucleinopathy disorders. Many of these assays, however, do not cross-react with mouse α-synuclein or exhibit poor sensitivity for this target, which is problematic considering the preponderance of mouse models at the forefront of pre-clinical α-synuclein research. In this project, we addressed this unmet need by reformulating two existing AlphaLISA<sup>®</sup> SureFire<sup>®</sup> Ultra™ total and pS129 α-synuclein assay kits to yield robust and ultrasensitive (LLoQ ≤ 0.5 pg/mL) quantification of mouse and human wild-type and pS129 α-synuclein protein. We then employed these assays, together with the BioLegend α-synuclein aggregate ELISA, to assess α-synuclein S129 phosphorylation and aggregation in different mouse brain tissue preparations. Overall, we highlight the compatibility of these new immunoassays with rodent models and demonstrate their potential to advance knowledge surrounding α-synuclein phosphorylation and aggregation in synucleinopathies.</p>}},
  author       = {{Trist, Benjamin Guy and Wright, Courtney Jade and Rangel, Alejandra and Cottle, Louise and Prasad, Asheeta and Jensen, Nanna Møller and Gram, Hjalte and Dzamko, Nicolas and Jensen, Poul Henning and Kirik, Deniz}},
  issn         = {{2373-8057}},
  language     = {{eng}},
  number       = {{1}},
  publisher    = {{Springer Nature}},
  series       = {{npj Parkinson's Disease}},
  title        = {{Novel tools to quantify total, phospho-Ser129 and aggregated alpha-synuclein in the mouse brain}},
  url          = {{http://dx.doi.org/10.1038/s41531-024-00830-y}},
  doi          = {{10.1038/s41531-024-00830-y}},
  volume       = {{10}},
  year         = {{2024}},
}