Gemini surfactants: New synthetic vectors for gene transfection
(2003) In Angewandte Chemie International Edition in English 42(13). p.1448-1457- Abstract
- The superior surfactant properties of cationic gemini surfactants are applied to the complex problem of introducing genes into cells. Of almost 250 new compounds tested, of some 20 different structural types, a majority showed very good transfection activity in vitro. The surfactant is shown to bind and compact DNA efficiently, and structural studies and calculations provide a working picture of the lipoplex formed. The lipoplex can penetrate the outer membranes of many cell types, to appear in the cytoplasm encapsulated within endosomes. Escape from the endosome - a key step for transfection - may be controlled by changes in the aggregation behavior of the lipoplex as the pH falls. The evidence suggests that DNA may be released from the... (More)
- The superior surfactant properties of cationic gemini surfactants are applied to the complex problem of introducing genes into cells. Of almost 250 new compounds tested, of some 20 different structural types, a majority showed very good transfection activity in vitro. The surfactant is shown to bind and compact DNA efficiently, and structural studies and calculations provide a working picture of the lipoplex formed. The lipoplex can penetrate the outer membranes of many cell types, to appear in the cytoplasm encapsulated within endosomes. Escape from the endosome - a key step for transfection - may be controlled by changes in the aggregation behavior of the lipoplex as the pH falls. The evidence suggests that DNA may be released from the lipoplex before entry into the nucleus, where the new gene can be expressed with high efficiency. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/122051
- author
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Angewandte Chemie International Edition in English
- volume
- 42
- issue
- 13
- pages
- 1448 - 1457
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000182337000003
- pmid:12698476
- scopus:0345352681
- ISSN
- 0570-0833
- DOI
- 10.1002/anie.200201597
- language
- English
- LU publication?
- yes
- id
- 960ecbd5-3abe-438b-82a2-ad73abf08b64 (old id 122051)
- date added to LUP
- 2016-04-01 16:25:44
- date last changed
- 2025-04-04 13:54:23
@article{960ecbd5-3abe-438b-82a2-ad73abf08b64,
abstract = {{The superior surfactant properties of cationic gemini surfactants are applied to the complex problem of introducing genes into cells. Of almost 250 new compounds tested, of some 20 different structural types, a majority showed very good transfection activity in vitro. The surfactant is shown to bind and compact DNA efficiently, and structural studies and calculations provide a working picture of the lipoplex formed. The lipoplex can penetrate the outer membranes of many cell types, to appear in the cytoplasm encapsulated within endosomes. Escape from the endosome - a key step for transfection - may be controlled by changes in the aggregation behavior of the lipoplex as the pH falls. The evidence suggests that DNA may be released from the lipoplex before entry into the nucleus, where the new gene can be expressed with high efficiency.}},
author = {{Kirby, A J and Camilleri, P and Engberts, J and Feiters, M. C. and Nolte, R J M and Söderman, Olle and Bergsma, M and Bell, P C and Fielden, M L and Rodriguez, C L G and Guedat, P and Kremer, A and McGregor, C and Perrin, C and Ronsin, G and van Eijk, Marcel C P}},
issn = {{0570-0833}},
language = {{eng}},
number = {{13}},
pages = {{1448--1457}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Angewandte Chemie International Edition in English}},
title = {{Gemini surfactants: New synthetic vectors for gene transfection}},
url = {{http://dx.doi.org/10.1002/anie.200201597}},
doi = {{10.1002/anie.200201597}},
volume = {{42}},
year = {{2003}},
}