Modification of adenosine A1 and A2A receptor density in the hippocampus of streptozotocin-induced diabetic rats
Duarte, João M N LU
; Oliveira, Catarina R
; Ambrósio, António F
and Cunha, Rodrigo A
(2006)
In Neurochemistry International
48(2).
p.144-150
- Abstract
Adenosine A(1) and A(2A) receptors are neuromodulatory systems that can control mnemonic behavior, which is modified by diabetes. Since the density of these adenosine receptors can change upon chronic noxious brain conditions, we now tested if the density of A(1) and A(2A) receptors was modified in the hippocampus of streptozotocin-induced diabetic rats. The binding density of the selective A(1) receptor antagonist, (3)H-DPCPX, was decreased by 36% in total hippocampal membranes 7 days after induction of diabetes and this down-regulation was maintained after 30 and 90 days, which was also confirmed by Western blot analysis of A(1) receptor immunoreactivity. In contrast, the binding density of the selective A(2A) receptor antagonist,... (More)
Adenosine A(1) and A(2A) receptors are neuromodulatory systems that can control mnemonic behavior, which is modified by diabetes. Since the density of these adenosine receptors can change upon chronic noxious brain conditions, we now tested if the density of A(1) and A(2A) receptors was modified in the hippocampus of streptozotocin-induced diabetic rats. The binding density of the selective A(1) receptor antagonist, (3)H-DPCPX, was decreased by 36% in total hippocampal membranes 7 days after induction of diabetes and this down-regulation was maintained after 30 and 90 days, which was also confirmed by Western blot analysis of A(1) receptor immunoreactivity. In contrast, the binding density of the selective A(2A) receptor antagonist, (3)H-SCH 58261, was enhanced by 83% in total hippocampal membranes 7 days after induction of diabetes and this up-regulation persisted after 30 and 90 days. These results show that the balance between inhibitory A(1) and facilitatory A(2A) adenosine receptors is modified in the hippocampus of streptozotocin-induced diabetic rats. Thus, the most abundant A(1) receptors are down-regulated and there is an up-regulation of A(2A) receptors, suggesting a gain of function of hippocampal A(2A) receptors compared to A(1) receptors in diabetes, as has been observed in other chronic noxious brain conditions where A(2A) receptor blockade affords robust neuroprotection.
(Less)
- author
- Duarte, João M N
LU
; Oliveira, Catarina R
; Ambrósio, António F
and Cunha, Rodrigo A
- publishing date
- 2006-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Animals, Blotting, Western, Diabetes Mellitus, Experimental/metabolism, Hippocampus/metabolism, Male, Rats, Rats, Wistar, Receptor, Adenosine A1/metabolism, Receptor, Adenosine A2A/metabolism, Streptozocin
- in
- Neurochemistry International
- volume
- 48
- issue
- 2
- pages
- 7 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:16256246
- scopus:29244450466
- ISSN
- 0197-0186
- DOI
- 10.1016/j.neuint.2005.08.008
- language
- English
- LU publication?
- no
- id
- 96450d92-0fbf-4060-81b4-e00294f53002
- date added to LUP
- 2019-02-22 09:19:36
- date last changed
- 2024-01-30 12:19:11
@article{96450d92-0fbf-4060-81b4-e00294f53002,
abstract = {{<p>Adenosine A(1) and A(2A) receptors are neuromodulatory systems that can control mnemonic behavior, which is modified by diabetes. Since the density of these adenosine receptors can change upon chronic noxious brain conditions, we now tested if the density of A(1) and A(2A) receptors was modified in the hippocampus of streptozotocin-induced diabetic rats. The binding density of the selective A(1) receptor antagonist, (3)H-DPCPX, was decreased by 36% in total hippocampal membranes 7 days after induction of diabetes and this down-regulation was maintained after 30 and 90 days, which was also confirmed by Western blot analysis of A(1) receptor immunoreactivity. In contrast, the binding density of the selective A(2A) receptor antagonist, (3)H-SCH 58261, was enhanced by 83% in total hippocampal membranes 7 days after induction of diabetes and this up-regulation persisted after 30 and 90 days. These results show that the balance between inhibitory A(1) and facilitatory A(2A) adenosine receptors is modified in the hippocampus of streptozotocin-induced diabetic rats. Thus, the most abundant A(1) receptors are down-regulated and there is an up-regulation of A(2A) receptors, suggesting a gain of function of hippocampal A(2A) receptors compared to A(1) receptors in diabetes, as has been observed in other chronic noxious brain conditions where A(2A) receptor blockade affords robust neuroprotection.</p>}},
author = {{Duarte, João M N and Oliveira, Catarina R and Ambrósio, António F and Cunha, Rodrigo A}},
issn = {{0197-0186}},
keywords = {{Animals; Blotting, Western; Diabetes Mellitus, Experimental/metabolism; Hippocampus/metabolism; Male; Rats; Rats, Wistar; Receptor, Adenosine A1/metabolism; Receptor, Adenosine A2A/metabolism; Streptozocin}},
language = {{eng}},
number = {{2}},
pages = {{144--150}},
publisher = {{Elsevier}},
series = {{Neurochemistry International}},
title = {{Modification of adenosine A1 and A2A receptor density in the hippocampus of streptozotocin-induced diabetic rats}},
url = {{http://dx.doi.org/10.1016/j.neuint.2005.08.008}},
doi = {{10.1016/j.neuint.2005.08.008}},
volume = {{48}},
year = {{2006}},
}