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Rapid elimination by glomerular filtration of free prostate specific antigen and human kallikrein 2 after renal transplantation.

Bruun, Laila LU ; Ekberg, Henrik LU ; Björk, Thomas LU ; Lilja, Hans LU ; Höglund, Peter LU and Christensson, Anders LU (2004) In Journal of Urology 171(4). p.1432-1435
Abstract

PURPOSE: The low molecular mass and short half-life of free (f) prostate specific antigen (PSA) implies elimination from blood by glomerular filtration. In addition, patients with terminal renal failure have increased fPSA in serum but there have been sparse data reported on the rates and pathways of elimination of PSA complexes and human kallikrein 2 (hK2). We studied glomerular filtration dependent elimination of fPSA and hK2 in patients with renal insufficiency undergoing successful renal transplantation.

MATERIALS AND METHODS: We studied 14 patients with immediate onset of renal function after renal transplantation. Blood samples were obtained before and at regular intervals up to 160 hours after transplanted kidney... (More)

PURPOSE: The low molecular mass and short half-life of free (f) prostate specific antigen (PSA) implies elimination from blood by glomerular filtration. In addition, patients with terminal renal failure have increased fPSA in serum but there have been sparse data reported on the rates and pathways of elimination of PSA complexes and human kallikrein 2 (hK2). We studied glomerular filtration dependent elimination of fPSA and hK2 in patients with renal insufficiency undergoing successful renal transplantation.

MATERIALS AND METHODS: We studied 14 patients with immediate onset of renal function after renal transplantation. Blood samples were obtained before and at regular intervals up to 160 hours after transplanted kidney reperfusion. Measurements of fPSA, total PSA and hK2 were performed with immunofluorometric assays and complexed PSA was determined by a chemiluminiscence assay. Glomerular filtration rates were monitored by analyzing serum creatinine and cystatin C. NONMEM, a multivariate pharmacokinetic approach, was used to determine the elimination rates of fPSA and hK2 after renal transplantation.

RESULTS: Serum fPSA and hK2 but not PSA complexes, decreased rapidly after renal transplantation. Significant reductions in fPSA and hK2 were observed after only 16 and 8 hours, respectively. fPSA and hK2 showed similar elimination patterns, decreasing to 42% and 44% of their original levels compared to cystatin C, which was at 44% after 160 hours. The median half-lives of fPSA and hK2 were 17.4 and 11.5 hours, respectively.

CONCLUSIONS: These results verify the hypothesis that fPSA and hK2 are eliminated from the blood circulation by glomerular filtration and severe renal failure influences the levels of the 2 proteins in serum.

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Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Aged, Female, Glomerular Filtration Rate, Humans, Kidney Transplantation, Male, Middle Aged, Prostate-Specific Antigen/blood, Time Factors, Tissue Kallikreins/blood
in
Journal of Urology
volume
171
issue
4
pages
1432 - 1435
publisher
Lippincott Williams & Wilkins
external identifiers
  • wos:000220361200008
  • pmid:15017191
  • scopus:1642404531
ISSN
1527-3792
DOI
10.1097/01.ju.0000118580.19344.68
language
English
LU publication?
yes
id
964e22eb-8401-4d62-b45a-dc3caa4ec72f (old id 121305)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=15017191&dopt=Abstract
date added to LUP
2007-07-05 07:36:30
date last changed
2019-07-30 03:02:21
@article{964e22eb-8401-4d62-b45a-dc3caa4ec72f,
  abstract     = {<p>PURPOSE: The low molecular mass and short half-life of free (f) prostate specific antigen (PSA) implies elimination from blood by glomerular filtration. In addition, patients with terminal renal failure have increased fPSA in serum but there have been sparse data reported on the rates and pathways of elimination of PSA complexes and human kallikrein 2 (hK2). We studied glomerular filtration dependent elimination of fPSA and hK2 in patients with renal insufficiency undergoing successful renal transplantation.</p><p>MATERIALS AND METHODS: We studied 14 patients with immediate onset of renal function after renal transplantation. Blood samples were obtained before and at regular intervals up to 160 hours after transplanted kidney reperfusion. Measurements of fPSA, total PSA and hK2 were performed with immunofluorometric assays and complexed PSA was determined by a chemiluminiscence assay. Glomerular filtration rates were monitored by analyzing serum creatinine and cystatin C. NONMEM, a multivariate pharmacokinetic approach, was used to determine the elimination rates of fPSA and hK2 after renal transplantation.</p><p>RESULTS: Serum fPSA and hK2 but not PSA complexes, decreased rapidly after renal transplantation. Significant reductions in fPSA and hK2 were observed after only 16 and 8 hours, respectively. fPSA and hK2 showed similar elimination patterns, decreasing to 42% and 44% of their original levels compared to cystatin C, which was at 44% after 160 hours. The median half-lives of fPSA and hK2 were 17.4 and 11.5 hours, respectively.</p><p>CONCLUSIONS: These results verify the hypothesis that fPSA and hK2 are eliminated from the blood circulation by glomerular filtration and severe renal failure influences the levels of the 2 proteins in serum.</p>},
  author       = {Bruun, Laila and Ekberg, Henrik and Björk, Thomas and Lilja, Hans and Höglund, Peter and Christensson, Anders},
  issn         = {1527-3792},
  keyword      = {Aged,Female,Glomerular Filtration Rate,Humans,Kidney Transplantation,Male,Middle Aged,Prostate-Specific Antigen/blood,Time Factors,Tissue Kallikreins/blood},
  language     = {eng},
  number       = {4},
  pages        = {1432--1435},
  publisher    = {Lippincott Williams & Wilkins},
  series       = {Journal of Urology},
  title        = {Rapid elimination by glomerular filtration of free prostate specific antigen and human kallikrein 2 after renal transplantation.},
  url          = {http://dx.doi.org/10.1097/01.ju.0000118580.19344.68},
  volume       = {171},
  year         = {2004},
}