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Surfactant-DNA gel particles: Formation and release characteristics

Moran, M. Carmen; Miguel, M. Graca and Lindman, Björn LU (2007) In Biomacromolecules 8(12). p.3886-3892
Abstract
Aqueous mixtures of oppositely charged polyelectrolytes undergo associative phase separation, resulting in coacervation, gelation, or precipitation. This phenomenon has been exploited here to form DNA gel particles by interfacial diffusion. We report on the formation of DNA gel particles by mixing solutions of DNA (either single-stranded (ssDNA) or double-stranded (dsDNA)) with solutions of cationic surfactant cetyltrimetrylammonium bromide (CTAB). By using CTAB, the formation of DNA reservoir gel particles, without adding any kind of cross-linker or organic solvent, has been demonstrated. Particles have been characterized with respect to the degree of DNA entrapment, surface morphology, and secondary structure of DNA in the particles. The... (More)
Aqueous mixtures of oppositely charged polyelectrolytes undergo associative phase separation, resulting in coacervation, gelation, or precipitation. This phenomenon has been exploited here to form DNA gel particles by interfacial diffusion. We report on the formation of DNA gel particles by mixing solutions of DNA (either single-stranded (ssDNA) or double-stranded (dsDNA)) with solutions of cationic surfactant cetyltrimetrylammonium bromide (CTAB). By using CTAB, the formation of DNA reservoir gel particles, without adding any kind of cross-linker or organic solvent, has been demonstrated. Particles have been characterized with respect to the degree of DNA entrapment, surface morphology, and secondary structure of DNA in the particles. The swelling/ deswelling behavior and the DNA release have been investigated in response to salt additions. Analysis of the data has suggested a higher degree of interaction between ssDNA and the cationic surfactant, confirming the stronger amphiphilic character of the denatured DNA. Fluorescence microscopy studies have suggested that the formation of these particles is associated with a conservation of the secondary structure of DNA. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biomacromolecules
volume
8
issue
12
pages
3886 - 3892
publisher
The American Chemical Society
external identifiers
  • wos:000251547600029
  • scopus:38049036826
ISSN
1526-4602
DOI
10.1021/bm700850z
language
English
LU publication?
yes
id
e8a1a20c-6460-4360-9f7d-b898182690d8 (old id 966414)
date added to LUP
2008-01-30 07:04:07
date last changed
2017-08-27 04:16:07
@article{e8a1a20c-6460-4360-9f7d-b898182690d8,
  abstract     = {Aqueous mixtures of oppositely charged polyelectrolytes undergo associative phase separation, resulting in coacervation, gelation, or precipitation. This phenomenon has been exploited here to form DNA gel particles by interfacial diffusion. We report on the formation of DNA gel particles by mixing solutions of DNA (either single-stranded (ssDNA) or double-stranded (dsDNA)) with solutions of cationic surfactant cetyltrimetrylammonium bromide (CTAB). By using CTAB, the formation of DNA reservoir gel particles, without adding any kind of cross-linker or organic solvent, has been demonstrated. Particles have been characterized with respect to the degree of DNA entrapment, surface morphology, and secondary structure of DNA in the particles. The swelling/ deswelling behavior and the DNA release have been investigated in response to salt additions. Analysis of the data has suggested a higher degree of interaction between ssDNA and the cationic surfactant, confirming the stronger amphiphilic character of the denatured DNA. Fluorescence microscopy studies have suggested that the formation of these particles is associated with a conservation of the secondary structure of DNA.},
  author       = {Moran, M. Carmen and Miguel, M. Graca and Lindman, Björn},
  issn         = {1526-4602},
  language     = {eng},
  number       = {12},
  pages        = {3886--3892},
  publisher    = {The American Chemical Society},
  series       = {Biomacromolecules},
  title        = {Surfactant-DNA gel particles: Formation and release characteristics},
  url          = {http://dx.doi.org/10.1021/bm700850z},
  volume       = {8},
  year         = {2007},
}